Additional information on the cryo-electron tomography performed on RNG2-mAID strain.
<p><b>(A)</b> Tomogram slices presented in <a href="http://www.plosbiology.org/article/info:doi/10.1371/journal.pbio.3003506#pbio.3003506.g004" target="_blank">Fig 4</a> without any overlay <b>(B)</b> Tomogram slices with and without over...
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2025
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| _version_ | 1849927642246021120 |
|---|---|
| author | Romuald Haase (22339777) |
| author2 | Bingjian Ren (22676577) Albert Tell i Puig (22676580) Alessandro Bonavoglia (19415718) Jean-Baptiste Marq (375239) Rémy Visentin (22676583) Nicolas Dos Santos Pacheco (22676586) Bohumil Maco (383443) Ricardo Mondragón-Flores (10737057) Oscar Vadas (2416228) Dominique Soldati-Favre (79026) |
| author2_role | author author author author author author author author author author |
| author_facet | Romuald Haase (22339777) Bingjian Ren (22676577) Albert Tell i Puig (22676580) Alessandro Bonavoglia (19415718) Jean-Baptiste Marq (375239) Rémy Visentin (22676583) Nicolas Dos Santos Pacheco (22676586) Bohumil Maco (383443) Ricardo Mondragón-Flores (10737057) Oscar Vadas (2416228) Dominique Soldati-Favre (79026) |
| author_role | author |
| dc.creator.none.fl_str_mv | Romuald Haase (22339777) Bingjian Ren (22676577) Albert Tell i Puig (22676580) Alessandro Bonavoglia (19415718) Jean-Baptiste Marq (375239) Rémy Visentin (22676583) Nicolas Dos Santos Pacheco (22676586) Bohumil Maco (383443) Ricardo Mondragón-Flores (10737057) Oscar Vadas (2416228) Dominique Soldati-Favre (79026) |
| dc.date.none.fl_str_mv | 2025-11-24T18:30:53Z |
| dc.identifier.none.fl_str_mv | 10.1371/journal.pbio.3003506.s005 |
| dc.relation.none.fl_str_mv | https://figshare.com/articles/figure/Additional_information_on_the_cryo-electron_tomography_performed_on_RNG2-mAID_strain_/30697326 |
| dc.rights.none.fl_str_mv | CC BY 4.0 info:eu-repo/semantics/openAccess |
| dc.subject.none.fl_str_mv | Biophysics Biochemistry Microbiology Cell Biology Physiology Infectious Diseases Environmental Sciences not elsewhere classified Biological Sciences not elsewhere classified Physical Sciences not elsewhere classified toxoplasma gondii </ remains apically anchored heterologous expression systems div >< p apical polar ring biochemical analysis revealed proper conoid anchorage ensures conoid functionality less parasites revealed host cell invasion enable parasite motility controlling parasite motility although rng2 depletion intact conoid organelle organelle traverses intact protein conditional depletion biochemical properties detached conoid gliding motility unstable protein undergoes extrusion two structures strong candidate striking detachment still unclear rng2 tethers rng2 led rng2 emerges rhoptries follow resilient bridge protein localized plasma membrane pivotal protein likely facilitate large coiled immunoelectron microscopy functional activity enabling rng2 electron tomography dependent extrusion critical role concatenated assemblies comprehensive mutagenesis coil domains calcium waves |
| dc.title.none.fl_str_mv | Additional information on the cryo-electron tomography performed on RNG2-mAID strain. |
| dc.type.none.fl_str_mv | Image Figure info:eu-repo/semantics/publishedVersion image |
| description | <p><b>(A)</b> Tomogram slices presented in <a href="http://www.plosbiology.org/article/info:doi/10.1371/journal.pbio.3003506#pbio.3003506.g004" target="_blank">Fig 4</a> without any overlay <b>(B)</b> Tomogram slices with and without overlay of untreated RNG2-mAID showing the correct positioning of the elements of the apical complex. <b>(C)</b> IFA images showing the localization of the Nd6 protein in presence or absence of RNG2. <b>(D)</b> U-ExM images showing the localization of the Nd6 protein in with the conoid attached or detached from the apical pole of the parasite.</p> <p>(TIFF)</p> |
| eu_rights_str_mv | openAccess |
| id | Manara_336039d2ba119f817b746d1103b7c520 |
| identifier_str_mv | 10.1371/journal.pbio.3003506.s005 |
| network_acronym_str | Manara |
| network_name_str | ManaraRepo |
| oai_identifier_str | oai:figshare.com:article/30697326 |
| publishDate | 2025 |
| repository.mail.fl_str_mv | |
| repository.name.fl_str_mv | |
| repository_id_str | |
| rights_invalid_str_mv | CC BY 4.0 |
| spelling | Additional information on the cryo-electron tomography performed on RNG2-mAID strain.Romuald Haase (22339777)Bingjian Ren (22676577)Albert Tell i Puig (22676580)Alessandro Bonavoglia (19415718)Jean-Baptiste Marq (375239)Rémy Visentin (22676583)Nicolas Dos Santos Pacheco (22676586)Bohumil Maco (383443)Ricardo Mondragón-Flores (10737057)Oscar Vadas (2416228)Dominique Soldati-Favre (79026)BiophysicsBiochemistryMicrobiologyCell BiologyPhysiologyInfectious DiseasesEnvironmental Sciences not elsewhere classifiedBiological Sciences not elsewhere classifiedPhysical Sciences not elsewhere classifiedtoxoplasma gondii </remains apically anchoredheterologous expression systemsdiv >< papical polar ringbiochemical analysis revealedproper conoid anchorageensures conoid functionalityless parasites revealedhost cell invasionenable parasite motilitycontrolling parasite motilityalthough rng2 depletionintact conoid organelleorganelle traversesintact proteinconditional depletionbiochemical propertiesdetached conoidgliding motilityunstable proteinundergoes extrusiontwo structuresstrong candidatestriking detachmentstill unclearrng2 tethersrng2 ledrng2 emergesrhoptries followresilient bridgeprotein localizedplasma membranepivotal proteinlikely facilitatelarge coiledimmunoelectron microscopyfunctional activityenabling rng2electron tomographydependent extrusioncritical roleconcatenated assembliescomprehensive mutagenesiscoil domainscalcium waves<p><b>(A)</b> Tomogram slices presented in <a href="http://www.plosbiology.org/article/info:doi/10.1371/journal.pbio.3003506#pbio.3003506.g004" target="_blank">Fig 4</a> without any overlay <b>(B)</b> Tomogram slices with and without overlay of untreated RNG2-mAID showing the correct positioning of the elements of the apical complex. <b>(C)</b> IFA images showing the localization of the Nd6 protein in presence or absence of RNG2. <b>(D)</b> U-ExM images showing the localization of the Nd6 protein in with the conoid attached or detached from the apical pole of the parasite.</p> <p>(TIFF)</p>2025-11-24T18:30:53ZImageFigureinfo:eu-repo/semantics/publishedVersionimage10.1371/journal.pbio.3003506.s005https://figshare.com/articles/figure/Additional_information_on_the_cryo-electron_tomography_performed_on_RNG2-mAID_strain_/30697326CC BY 4.0info:eu-repo/semantics/openAccessoai:figshare.com:article/306973262025-11-24T18:30:53Z |
| spellingShingle | Additional information on the cryo-electron tomography performed on RNG2-mAID strain. Romuald Haase (22339777) Biophysics Biochemistry Microbiology Cell Biology Physiology Infectious Diseases Environmental Sciences not elsewhere classified Biological Sciences not elsewhere classified Physical Sciences not elsewhere classified toxoplasma gondii </ remains apically anchored heterologous expression systems div >< p apical polar ring biochemical analysis revealed proper conoid anchorage ensures conoid functionality less parasites revealed host cell invasion enable parasite motility controlling parasite motility although rng2 depletion intact conoid organelle organelle traverses intact protein conditional depletion biochemical properties detached conoid gliding motility unstable protein undergoes extrusion two structures strong candidate striking detachment still unclear rng2 tethers rng2 led rng2 emerges rhoptries follow resilient bridge protein localized plasma membrane pivotal protein likely facilitate large coiled immunoelectron microscopy functional activity enabling rng2 electron tomography dependent extrusion critical role concatenated assemblies comprehensive mutagenesis coil domains calcium waves |
| status_str | publishedVersion |
| title | Additional information on the cryo-electron tomography performed on RNG2-mAID strain. |
| title_full | Additional information on the cryo-electron tomography performed on RNG2-mAID strain. |
| title_fullStr | Additional information on the cryo-electron tomography performed on RNG2-mAID strain. |
| title_full_unstemmed | Additional information on the cryo-electron tomography performed on RNG2-mAID strain. |
| title_short | Additional information on the cryo-electron tomography performed on RNG2-mAID strain. |
| title_sort | Additional information on the cryo-electron tomography performed on RNG2-mAID strain. |
| topic | Biophysics Biochemistry Microbiology Cell Biology Physiology Infectious Diseases Environmental Sciences not elsewhere classified Biological Sciences not elsewhere classified Physical Sciences not elsewhere classified toxoplasma gondii </ remains apically anchored heterologous expression systems div >< p apical polar ring biochemical analysis revealed proper conoid anchorage ensures conoid functionality less parasites revealed host cell invasion enable parasite motility controlling parasite motility although rng2 depletion intact conoid organelle organelle traverses intact protein conditional depletion biochemical properties detached conoid gliding motility unstable protein undergoes extrusion two structures strong candidate striking detachment still unclear rng2 tethers rng2 led rng2 emerges rhoptries follow resilient bridge protein localized plasma membrane pivotal protein likely facilitate large coiled immunoelectron microscopy functional activity enabling rng2 electron tomography dependent extrusion critical role concatenated assemblies comprehensive mutagenesis coil domains calcium waves |