Conidiation and conidial morphology of the SH3 domain-containing gene mutants.
<p><b>(A)</b> Conidia production assay of the various mutants. The conidia were harvested from carboxymethyl cellulose (CMC) liquid media after four days of agitation in an orbital shaker operating 200 rpm, 28°C. The values presented represent mean ± SD. Each strain was counted fiv...
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2025
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| _version_ | 1849927641153404928 |
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| author | Yakubu Saddeeq Abubakar (5558399) |
| author2 | Shiqi Ji (20476509) Qiaoli Chen (538344) Huawei Zheng (713784) Zonghua Wang (133934) Wenhui Zheng (133904) Yi Lou (381696) |
| author2_role | author author author author author author |
| author_facet | Yakubu Saddeeq Abubakar (5558399) Shiqi Ji (20476509) Qiaoli Chen (538344) Huawei Zheng (713784) Zonghua Wang (133934) Wenhui Zheng (133904) Yi Lou (381696) |
| author_role | author |
| dc.creator.none.fl_str_mv | Yakubu Saddeeq Abubakar (5558399) Shiqi Ji (20476509) Qiaoli Chen (538344) Huawei Zheng (713784) Zonghua Wang (133934) Wenhui Zheng (133904) Yi Lou (381696) |
| dc.date.none.fl_str_mv | 2025-11-24T18:36:16Z |
| dc.identifier.none.fl_str_mv | 10.1371/journal.ppat.1013726.g002 |
| dc.relation.none.fl_str_mv | https://figshare.com/articles/figure/Conidiation_and_conidial_morphology_of_the_SH3_domain-containing_gene_mutants_/30697898 |
| dc.rights.none.fl_str_mv | CC BY 4.0 info:eu-repo/semantics/openAccess |
| dc.subject.none.fl_str_mv | Biophysics Biochemistry Medicine Microbiology Cell Biology Genetics Developmental Biology Infectious Diseases Plant Biology div >< p conserved molecular scaffolds irregular colony edges form protein condensates dynamic protein assembly remaining 20 proteins plant fungal pathogen idrs largely altered proteins exhibited features fusarium graminearum </ graminearum </ fusarium </ protein interaction plant infection mediate protein irregular septation fungal pathogenesis containing proteins wide characterization varied functions systematically identified src homology septal pore previously characterized potential targets plasma membrane nuclear fragmentation nuclear distribution negative regulators mutant strains multifaceted functions hyphal tip head blight functional diversity completely abolished apical collar |
| dc.title.none.fl_str_mv | Conidiation and conidial morphology of the SH3 domain-containing gene mutants. |
| dc.type.none.fl_str_mv | Image Figure info:eu-repo/semantics/publishedVersion image |
| description | <p><b>(A)</b> Conidia production assay of the various mutants. The conidia were harvested from carboxymethyl cellulose (CMC) liquid media after four days of agitation in an orbital shaker operating 200 rpm, 28°C. The values presented represent mean ± SD. Each strain was counted five times. Bars with asterisks are significantly (p < 0.05) different from the wild-type PH-1. ns: non-significant. <b>(B)</b> The spore morphology of the indicated strains. The spores were harvested from four-day-old CMC cultures, stained with CFW and observed under a confocal microscope. CFW: calcofluor white; DIC: differential interference contrast. Bar = 10 µm<b>. (C)</b> The number of septa present in the spores produced by the SH3 domain-containing gene mutants were quantified and compared. About 200 spores were involved in the septation assay from each mutant. Three independent experiments were conducted.</p> |
| eu_rights_str_mv | openAccess |
| id | Manara_344208c6eb6ce908bf8614c8568f1228 |
| identifier_str_mv | 10.1371/journal.ppat.1013726.g002 |
| network_acronym_str | Manara |
| network_name_str | ManaraRepo |
| oai_identifier_str | oai:figshare.com:article/30697898 |
| publishDate | 2025 |
| repository.mail.fl_str_mv | |
| repository.name.fl_str_mv | |
| repository_id_str | |
| rights_invalid_str_mv | CC BY 4.0 |
| spelling | Conidiation and conidial morphology of the SH3 domain-containing gene mutants.Yakubu Saddeeq Abubakar (5558399)Shiqi Ji (20476509)Qiaoli Chen (538344)Huawei Zheng (713784)Zonghua Wang (133934)Wenhui Zheng (133904)Yi Lou (381696)BiophysicsBiochemistryMedicineMicrobiologyCell BiologyGeneticsDevelopmental BiologyInfectious DiseasesPlant Biologydiv >< pconserved molecular scaffoldsirregular colony edgesform protein condensatesdynamic protein assemblyremaining 20 proteinsplant fungal pathogenidrs largely alteredproteins exhibited featuresfusarium graminearum </graminearum </fusarium </protein interactionplant infectionmediate proteinirregular septationfungal pathogenesiscontaining proteinswide characterizationvaried functionssystematically identifiedsrc homologyseptal porepreviously characterizedpotential targetsplasma membranenuclear fragmentationnuclear distributionnegative regulatorsmutant strainsmultifaceted functionshyphal tiphead blightfunctional diversitycompletely abolishedapical collar<p><b>(A)</b> Conidia production assay of the various mutants. The conidia were harvested from carboxymethyl cellulose (CMC) liquid media after four days of agitation in an orbital shaker operating 200 rpm, 28°C. The values presented represent mean ± SD. Each strain was counted five times. Bars with asterisks are significantly (p < 0.05) different from the wild-type PH-1. ns: non-significant. <b>(B)</b> The spore morphology of the indicated strains. The spores were harvested from four-day-old CMC cultures, stained with CFW and observed under a confocal microscope. CFW: calcofluor white; DIC: differential interference contrast. Bar = 10 µm<b>. (C)</b> The number of septa present in the spores produced by the SH3 domain-containing gene mutants were quantified and compared. About 200 spores were involved in the septation assay from each mutant. Three independent experiments were conducted.</p>2025-11-24T18:36:16ZImageFigureinfo:eu-repo/semantics/publishedVersionimage10.1371/journal.ppat.1013726.g002https://figshare.com/articles/figure/Conidiation_and_conidial_morphology_of_the_SH3_domain-containing_gene_mutants_/30697898CC BY 4.0info:eu-repo/semantics/openAccessoai:figshare.com:article/306978982025-11-24T18:36:16Z |
| spellingShingle | Conidiation and conidial morphology of the SH3 domain-containing gene mutants. Yakubu Saddeeq Abubakar (5558399) Biophysics Biochemistry Medicine Microbiology Cell Biology Genetics Developmental Biology Infectious Diseases Plant Biology div >< p conserved molecular scaffolds irregular colony edges form protein condensates dynamic protein assembly remaining 20 proteins plant fungal pathogen idrs largely altered proteins exhibited features fusarium graminearum </ graminearum </ fusarium </ protein interaction plant infection mediate protein irregular septation fungal pathogenesis containing proteins wide characterization varied functions systematically identified src homology septal pore previously characterized potential targets plasma membrane nuclear fragmentation nuclear distribution negative regulators mutant strains multifaceted functions hyphal tip head blight functional diversity completely abolished apical collar |
| status_str | publishedVersion |
| title | Conidiation and conidial morphology of the SH3 domain-containing gene mutants. |
| title_full | Conidiation and conidial morphology of the SH3 domain-containing gene mutants. |
| title_fullStr | Conidiation and conidial morphology of the SH3 domain-containing gene mutants. |
| title_full_unstemmed | Conidiation and conidial morphology of the SH3 domain-containing gene mutants. |
| title_short | Conidiation and conidial morphology of the SH3 domain-containing gene mutants. |
| title_sort | Conidiation and conidial morphology of the SH3 domain-containing gene mutants. |
| topic | Biophysics Biochemistry Medicine Microbiology Cell Biology Genetics Developmental Biology Infectious Diseases Plant Biology div >< p conserved molecular scaffolds irregular colony edges form protein condensates dynamic protein assembly remaining 20 proteins plant fungal pathogen idrs largely altered proteins exhibited features fusarium graminearum </ graminearum </ fusarium </ protein interaction plant infection mediate protein irregular septation fungal pathogenesis containing proteins wide characterization varied functions systematically identified src homology septal pore previously characterized potential targets plasma membrane nuclear fragmentation nuclear distribution negative regulators mutant strains multifaceted functions hyphal tip head blight functional diversity completely abolished apical collar |