Supplementary file 1_The biotechnological potential of bacterial extracellular polymeric substances in lead biosorption.docx
Introduction<p>Extracellular polymeric substances are composed of a diverse range of functional groups, thereby making a strong case for their consideration as biosorbents in heavy metal bioremediation. This study, therefore, assessed strategies to enhance the biotechnological potential of ext...
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2025
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| _version_ | 1849927621831294976 |
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| author | Pfariso Maumela (18462231) |
| author2 | Mahloro Serepa-Dlamimi (22688090) |
| author2_role | author |
| author_facet | Pfariso Maumela (18462231) Mahloro Serepa-Dlamimi (22688090) |
| author_role | author |
| dc.creator.none.fl_str_mv | Pfariso Maumela (18462231) Mahloro Serepa-Dlamimi (22688090) |
| dc.date.none.fl_str_mv | 2025-11-26T08:40:50Z |
| dc.identifier.none.fl_str_mv | 10.3389/fmicb.2025.1650222.s001 |
| dc.relation.none.fl_str_mv | https://figshare.com/articles/dataset/Supplementary_file_1_The_biotechnological_potential_of_bacterial_extracellular_polymeric_substances_in_lead_biosorption_docx/30719513 |
| dc.rights.none.fl_str_mv | CC BY 4.0 info:eu-repo/semantics/openAccess |
| dc.subject.none.fl_str_mv | Microbiology Bacillus carboxyl FTIR lead hydrophobicity |
| dc.title.none.fl_str_mv | Supplementary file 1_The biotechnological potential of bacterial extracellular polymeric substances in lead biosorption.docx |
| dc.type.none.fl_str_mv | Dataset info:eu-repo/semantics/publishedVersion dataset |
| description | Introduction<p>Extracellular polymeric substances are composed of a diverse range of functional groups, thereby making a strong case for their consideration as biosorbents in heavy metal bioremediation. This study, therefore, assessed strategies to enhance the biotechnological potential of extracellular polymeric substances produced by an endophytic bacterium, Bacillus MHSD_36.</p>Methods<p>Design of experiments were used to optimize the yield of extracellular polymeric substances from Bacillus MHSD_36. A mixture design was subsequently used to develop a cocktail of EPS and hydrophobicity components for the optimal biosorption of lead.</p>Results<p>The production of the EPS, from MHSD_36, was optimized through lead induction at a concentration and time of 5.23 mg/L and 10.75 h, respectively. The optimum yield was 1.65 g/L EPS. The use of garden compost, as an alternative growth medium, was sufficient to give an EPS yield (1.15 g/L) comparable to sucrose based medium (1.25 g/L) under optimal induction conditions. The EPS from the Bacillus MHSD_36 had a Pb biosorption of 14.24%. However, a mixture of EPS with the hydrophobicity components significantly enhanced the Pb biosorption. The optimal proportion for the mixture was estimated to be 0.25 and 0.75, respectively with a maximum Pb biosorption of 95.8%. The acid recovery of EPS after the biosorption was effective to recover and recycle EPS in heavy metal biosorption.</p>Discussion<p>The production of EPS using garden compost and the subsequent recovery after biosorption of heavy metal offers a sustainable approach for the biotechnological application of bacterial EPS in environmental bioremediation.</p> |
| eu_rights_str_mv | openAccess |
| id | Manara_386e07efde80bfd013872c01de21a07c |
| identifier_str_mv | 10.3389/fmicb.2025.1650222.s001 |
| network_acronym_str | Manara |
| network_name_str | ManaraRepo |
| oai_identifier_str | oai:figshare.com:article/30719513 |
| publishDate | 2025 |
| repository.mail.fl_str_mv | |
| repository.name.fl_str_mv | |
| repository_id_str | |
| rights_invalid_str_mv | CC BY 4.0 |
| spelling | Supplementary file 1_The biotechnological potential of bacterial extracellular polymeric substances in lead biosorption.docxPfariso Maumela (18462231)Mahloro Serepa-Dlamimi (22688090)MicrobiologyBacilluscarboxylFTIRleadhydrophobicityIntroduction<p>Extracellular polymeric substances are composed of a diverse range of functional groups, thereby making a strong case for their consideration as biosorbents in heavy metal bioremediation. This study, therefore, assessed strategies to enhance the biotechnological potential of extracellular polymeric substances produced by an endophytic bacterium, Bacillus MHSD_36.</p>Methods<p>Design of experiments were used to optimize the yield of extracellular polymeric substances from Bacillus MHSD_36. A mixture design was subsequently used to develop a cocktail of EPS and hydrophobicity components for the optimal biosorption of lead.</p>Results<p>The production of the EPS, from MHSD_36, was optimized through lead induction at a concentration and time of 5.23 mg/L and 10.75 h, respectively. The optimum yield was 1.65 g/L EPS. The use of garden compost, as an alternative growth medium, was sufficient to give an EPS yield (1.15 g/L) comparable to sucrose based medium (1.25 g/L) under optimal induction conditions. The EPS from the Bacillus MHSD_36 had a Pb biosorption of 14.24%. However, a mixture of EPS with the hydrophobicity components significantly enhanced the Pb biosorption. The optimal proportion for the mixture was estimated to be 0.25 and 0.75, respectively with a maximum Pb biosorption of 95.8%. The acid recovery of EPS after the biosorption was effective to recover and recycle EPS in heavy metal biosorption.</p>Discussion<p>The production of EPS using garden compost and the subsequent recovery after biosorption of heavy metal offers a sustainable approach for the biotechnological application of bacterial EPS in environmental bioremediation.</p>2025-11-26T08:40:50ZDatasetinfo:eu-repo/semantics/publishedVersiondataset10.3389/fmicb.2025.1650222.s001https://figshare.com/articles/dataset/Supplementary_file_1_The_biotechnological_potential_of_bacterial_extracellular_polymeric_substances_in_lead_biosorption_docx/30719513CC BY 4.0info:eu-repo/semantics/openAccessoai:figshare.com:article/307195132025-11-26T08:40:50Z |
| spellingShingle | Supplementary file 1_The biotechnological potential of bacterial extracellular polymeric substances in lead biosorption.docx Pfariso Maumela (18462231) Microbiology Bacillus carboxyl FTIR lead hydrophobicity |
| status_str | publishedVersion |
| title | Supplementary file 1_The biotechnological potential of bacterial extracellular polymeric substances in lead biosorption.docx |
| title_full | Supplementary file 1_The biotechnological potential of bacterial extracellular polymeric substances in lead biosorption.docx |
| title_fullStr | Supplementary file 1_The biotechnological potential of bacterial extracellular polymeric substances in lead biosorption.docx |
| title_full_unstemmed | Supplementary file 1_The biotechnological potential of bacterial extracellular polymeric substances in lead biosorption.docx |
| title_short | Supplementary file 1_The biotechnological potential of bacterial extracellular polymeric substances in lead biosorption.docx |
| title_sort | Supplementary file 1_The biotechnological potential of bacterial extracellular polymeric substances in lead biosorption.docx |
| topic | Microbiology Bacillus carboxyl FTIR lead hydrophobicity |