Figure 3 from SMG8/SMG9 Heterodimer Loss Modulates SMG1 Kinase to Drive ATR Inhibitor Resistance
<p>Silencing of SMG1 resensitizes SMG8 and SMG9 knockout cells to ATR inhibition. <b>A,</b> Left, SMG1 was overexpressed in the SMG8 and SMG9 mutant cells after 48 hours of DMSO or 150 nmol/L berzosertib exposure compared with the WT cells. Right, Smg1 signal intensity relative to...
Kaydedildi:
| Yazar: | |
|---|---|
| Diğer Yazarlar: | , , , , , , , , , , , , , , , , , , , , , |
| Baskı/Yayın Bilgisi: |
2025
|
| Konular: | |
| Etiketler: |
Etiketle
Etiket eklenmemiş, İlk siz ekleyin!
|
| _version_ | 1849927639829053440 |
|---|---|
| author | Marta J. Llorca-Cardenosa (14961612) |
| author2 | Lauren I. Aronson (14961615) Dragomir B. Krastev (14961618) Jadwiga Nieminuszczy (14961621) John Alexander (14961624) Feifei Song (14961627) Malgorzata Dylewska (14961630) Ronan Broderick (14961633) Rachel Brough (14961636) Astrid Zimmermann (14961639) Frank T. Zenke (14961642) Bora Gurel (14961645) Ruth Riisnaes (14961648) Ana Ferreira (14961651) Theodoros Roumeliotis (14961654) Jyoti Choudhary (14961657) Stephen J. Pettitt (14952601) Johann de Bono (14961660) Andres Cervantes (14961663) Syed Haider (14961666) Wojciech Niedzwiedz (14961669) Christopher J. Lord (14952616) Irene Y. Chong (14961672) |
| author2_role | author author author author author author author author author author author author author author author author author author author author author author |
| author_facet | Marta J. Llorca-Cardenosa (14961612) Lauren I. Aronson (14961615) Dragomir B. Krastev (14961618) Jadwiga Nieminuszczy (14961621) John Alexander (14961624) Feifei Song (14961627) Malgorzata Dylewska (14961630) Ronan Broderick (14961633) Rachel Brough (14961636) Astrid Zimmermann (14961639) Frank T. Zenke (14961642) Bora Gurel (14961645) Ruth Riisnaes (14961648) Ana Ferreira (14961651) Theodoros Roumeliotis (14961654) Jyoti Choudhary (14961657) Stephen J. Pettitt (14952601) Johann de Bono (14961660) Andres Cervantes (14961663) Syed Haider (14961666) Wojciech Niedzwiedz (14961669) Christopher J. Lord (14952616) Irene Y. Chong (14961672) |
| author_role | author |
| dc.creator.none.fl_str_mv | Marta J. Llorca-Cardenosa (14961612) Lauren I. Aronson (14961615) Dragomir B. Krastev (14961618) Jadwiga Nieminuszczy (14961621) John Alexander (14961624) Feifei Song (14961627) Malgorzata Dylewska (14961630) Ronan Broderick (14961633) Rachel Brough (14961636) Astrid Zimmermann (14961639) Frank T. Zenke (14961642) Bora Gurel (14961645) Ruth Riisnaes (14961648) Ana Ferreira (14961651) Theodoros Roumeliotis (14961654) Jyoti Choudhary (14961657) Stephen J. Pettitt (14952601) Johann de Bono (14961660) Andres Cervantes (14961663) Syed Haider (14961666) Wojciech Niedzwiedz (14961669) Christopher J. Lord (14952616) Irene Y. Chong (14961672) |
| dc.date.none.fl_str_mv | 2025-11-24T22:40:24Z |
| dc.identifier.none.fl_str_mv | 10.1158/0008-5472.30699136 |
| dc.relation.none.fl_str_mv | https://figshare.com/articles/figure/Figure_3_from_SMG8_SMG9_Heterodimer_Loss_Modulates_SMG1_Kinase_to_Drive_ATR_Inhibitor_Resistance/30699136 |
| dc.rights.none.fl_str_mv | CC BY info:eu-repo/semantics/openAccess |
| dc.subject.none.fl_str_mv | Cancer Molecular and Cellular Biology Therapeutic Research and Development DNA Damage and Repair DNA repair Drug Mechanisms Drug Resistance Gastrointestinal Cancers Stomach cancer |
| dc.title.none.fl_str_mv | Figure 3 from SMG8/SMG9 Heterodimer Loss Modulates SMG1 Kinase to Drive ATR Inhibitor Resistance |
| dc.type.none.fl_str_mv | Image Figure info:eu-repo/semantics/publishedVersion image |
| description | <p>Silencing of SMG1 resensitizes SMG8 and SMG9 knockout cells to ATR inhibition. <b>A,</b> Left, SMG1 was overexpressed in the SMG8 and SMG9 mutant cells after 48 hours of DMSO or 150 nmol/L berzosertib exposure compared with the WT cells. Right, Smg1 signal intensity relative to β-actin expression representing relative protein expression using ImageJ. Error bars represent SEM, considering three biological replicates. <b>B</b> and <b>C,</b> SMG1 knockdown experiment (384-well plates) showing an increase in the normalized percent of inhibition in the SMG8 and SMG9 mutant cells (red) compared with the WT cells (blue) following exposure to a range of SMG1 siRNA concentrations (from 1 to 20 nmol/L; negative controls, siCON1, siCON2; positive controls, siATM, siPLK1). <b>D,</b> Western blot analysis showing siSMG1 silencing in the YCC6 WT and SMG8 and SMG9 mutant cells. <b>E–G,</b> Silencing of SMG1 in YCC6 WT, SMG8, and SMG9 mutant cells resensitizes them to ATRi (384-well plate, 5-day assay). siCON2 was used as a negative control. All panels of this figure are representative of three or more biological replicates. ns, nonsignificant.</p> |
| eu_rights_str_mv | openAccess |
| id | Manara_394c064207e5c4ee928953f745d0340c |
| identifier_str_mv | 10.1158/0008-5472.30699136 |
| network_acronym_str | Manara |
| network_name_str | ManaraRepo |
| oai_identifier_str | oai:figshare.com:article/30699136 |
| publishDate | 2025 |
| repository.mail.fl_str_mv | |
| repository.name.fl_str_mv | |
| repository_id_str | |
| rights_invalid_str_mv | CC BY |
| spelling | Figure 3 from SMG8/SMG9 Heterodimer Loss Modulates SMG1 Kinase to Drive ATR Inhibitor ResistanceMarta J. Llorca-Cardenosa (14961612)Lauren I. Aronson (14961615)Dragomir B. Krastev (14961618)Jadwiga Nieminuszczy (14961621)John Alexander (14961624)Feifei Song (14961627)Malgorzata Dylewska (14961630)Ronan Broderick (14961633)Rachel Brough (14961636)Astrid Zimmermann (14961639)Frank T. Zenke (14961642)Bora Gurel (14961645)Ruth Riisnaes (14961648)Ana Ferreira (14961651)Theodoros Roumeliotis (14961654)Jyoti Choudhary (14961657)Stephen J. Pettitt (14952601)Johann de Bono (14961660)Andres Cervantes (14961663)Syed Haider (14961666)Wojciech Niedzwiedz (14961669)Christopher J. Lord (14952616)Irene Y. Chong (14961672)CancerMolecular and Cellular BiologyTherapeutic Research and DevelopmentDNA Damage and RepairDNA repairDrug MechanismsDrug ResistanceGastrointestinal CancersStomach cancer<p>Silencing of SMG1 resensitizes SMG8 and SMG9 knockout cells to ATR inhibition. <b>A,</b> Left, SMG1 was overexpressed in the SMG8 and SMG9 mutant cells after 48 hours of DMSO or 150 nmol/L berzosertib exposure compared with the WT cells. Right, Smg1 signal intensity relative to β-actin expression representing relative protein expression using ImageJ. Error bars represent SEM, considering three biological replicates. <b>B</b> and <b>C,</b> SMG1 knockdown experiment (384-well plates) showing an increase in the normalized percent of inhibition in the SMG8 and SMG9 mutant cells (red) compared with the WT cells (blue) following exposure to a range of SMG1 siRNA concentrations (from 1 to 20 nmol/L; negative controls, siCON1, siCON2; positive controls, siATM, siPLK1). <b>D,</b> Western blot analysis showing siSMG1 silencing in the YCC6 WT and SMG8 and SMG9 mutant cells. <b>E–G,</b> Silencing of SMG1 in YCC6 WT, SMG8, and SMG9 mutant cells resensitizes them to ATRi (384-well plate, 5-day assay). siCON2 was used as a negative control. All panels of this figure are representative of three or more biological replicates. ns, nonsignificant.</p>2025-11-24T22:40:24ZImageFigureinfo:eu-repo/semantics/publishedVersionimage10.1158/0008-5472.30699136https://figshare.com/articles/figure/Figure_3_from_SMG8_SMG9_Heterodimer_Loss_Modulates_SMG1_Kinase_to_Drive_ATR_Inhibitor_Resistance/30699136CC BYinfo:eu-repo/semantics/openAccessoai:figshare.com:article/306991362025-11-24T22:40:24Z |
| spellingShingle | Figure 3 from SMG8/SMG9 Heterodimer Loss Modulates SMG1 Kinase to Drive ATR Inhibitor Resistance Marta J. Llorca-Cardenosa (14961612) Cancer Molecular and Cellular Biology Therapeutic Research and Development DNA Damage and Repair DNA repair Drug Mechanisms Drug Resistance Gastrointestinal Cancers Stomach cancer |
| status_str | publishedVersion |
| title | Figure 3 from SMG8/SMG9 Heterodimer Loss Modulates SMG1 Kinase to Drive ATR Inhibitor Resistance |
| title_full | Figure 3 from SMG8/SMG9 Heterodimer Loss Modulates SMG1 Kinase to Drive ATR Inhibitor Resistance |
| title_fullStr | Figure 3 from SMG8/SMG9 Heterodimer Loss Modulates SMG1 Kinase to Drive ATR Inhibitor Resistance |
| title_full_unstemmed | Figure 3 from SMG8/SMG9 Heterodimer Loss Modulates SMG1 Kinase to Drive ATR Inhibitor Resistance |
| title_short | Figure 3 from SMG8/SMG9 Heterodimer Loss Modulates SMG1 Kinase to Drive ATR Inhibitor Resistance |
| title_sort | Figure 3 from SMG8/SMG9 Heterodimer Loss Modulates SMG1 Kinase to Drive ATR Inhibitor Resistance |
| topic | Cancer Molecular and Cellular Biology Therapeutic Research and Development DNA Damage and Repair DNA repair Drug Mechanisms Drug Resistance Gastrointestinal Cancers Stomach cancer |