Data Sheet 1_Intratumoral microbial abundance and load influence the immune microenvironment of oral squamous cell carcinoma.xlsx
Objective<p>This study aimed to explore the characteristics intratumoral microbiome in oral squamous cell carcinoma, and elucidate the interplay between intratumoral microbial profiles (relative abundance/absolute load) and tumor-infiltrating lymphocytes markers (CD4<sup>+</sup>/CD...
محفوظ في:
| المؤلف الرئيسي: | |
|---|---|
| مؤلفون آخرون: | , , , |
| منشور في: |
2025
|
| الموضوعات: | |
| الوسوم: |
إضافة وسم
لا توجد وسوم, كن أول من يضع وسما على هذه التسجيلة!
|
| الملخص: | Objective<p>This study aimed to explore the characteristics intratumoral microbiome in oral squamous cell carcinoma, and elucidate the interplay between intratumoral microbial profiles (relative abundance/absolute load) and tumor-infiltrating lymphocytes markers (CD4<sup>+</sup>/CD8<sup>+</sup>/FOXP3) and PD-L1 in oral squamous cell carcinoma</p>Methods<p>We analyzed 45 OSCC tissue samples alongside paired paracancerous (n=10) and normal oral microbiota controls (n=8). Microbial composition was characterized by 16S rRNA sequencing (V3-V4 regions), with bacterial load quantified via qPCR targeting the V4–1 region. Tumor-infiltrating lymphocytes markers were assessed by immunohistochemistry.</p>Results<p>Spirochaetota was concentrated in the CD4<sup>+</sup> as well as CD8<sup>+</sup> low infiltration groups, Proteobacteria in the CD8<sup>+</sup> high infiltration group and Actinobacteriota in the FOXP3 low infiltration group. Tumor microbial load was negatively correlated with CD4<sup>+</sup>, CD8<sup>+</sup>, and FOXP3, but of these, only the correlation coefficient of -0.309 for CD4<sup>+</sup> was statistically significant. However, no significant correlation was observed in the analysis of PD-L1 expression with the relative abundance of intratumoral microbiome, α-diversity, and intratumoral microbial load values</p>Conclusion<p>Changes in the abundance of specific intratumoral microbiome affect the infiltration of TILs markers, and there is a negative relationship between intratumoral microbial load and T-cell infiltration, suggesting that intratumoral microbiome contribute to the processes of the tumor immunosuppressive microenvironment.</p> |
|---|