Figure 4 from Translational Activation of ATF4 through Mitochondrial Anaplerotic Metabolic Pathways Is Required for DLBCL Growth and Survival
<p>ATF4 protein level is decreased in <i>vavP-Bcl2;Sirt3</i><sup>−/−</sup> mice and associated with lymphoma progression. <b>A,</b> Western blot results show the protein levels of ATF4, LC3, EIF2A, ACTB, and SIRT3 in splenocytes from <i>vavP-Bcl2;Sirt3...
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2025
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| Итог: | <p>ATF4 protein level is decreased in <i>vavP-Bcl2;Sirt3</i><sup>−/−</sup> mice and associated with lymphoma progression. <b>A,</b> Western blot results show the protein levels of ATF4, LC3, EIF2A, ACTB, and SIRT3 in splenocytes from <i>vavP-Bcl2;Sirt3</i><sup>+/+</sup> and <i>vavP-Bcl2;Sirt3</i><sup>−/−</sup> mice. The protein amounts were quantified with densitometry results. <b>B,</b> Summarized results of ATF4 protein level normalized to ACTB in splenocytes from <i>vavP-Bcl2;Sirt3</i><sup>+/+</sup> and <i>vavP-Bcl2;Sirt3</i><sup>−/−</sup> mice. The protein amounts were quantified with densitometry results from Western blots. <b>C,</b> Correlation between levels of autophagy (LC3II/LC3I) and ATF4 (ATF4/ACTB) in splenocytes from <i>vavP-Bcl2;Sirt3</i><sup>+/+</sup> and <i>vavP-Bcl2;Sirt3</i><sup>−/−</sup> mice. The data for correlation study were obtained with densitometry results from Western blots. <b>D,</b> Correlation between levels of phospho-EIF2A (p-EIF2A/EIF2A) and ATF4 (ATF4/ACTB) in splenocytes from <i>vavP-Bcl2;Sirt3</i><sup>+/+</sup> and <i>vavP-Bcl2;Sirt3</i><sup>−/−</sup> mice. The data for correlation study were obtained with densitometry results from Western blots. <b>E,</b> Summarized results of phospho-EIF2A level normalized to total EIF2A in splenocytes from <i>vavP-Bcl2;Sirt3</i><sup>+/+</sup> and <i>vavP-Bcl2;Sirt3</i><sup>−/−</sup> mice. The protein amounts were quantified with densitometry results from Western blots. <b>F,</b> Correlation between splenomegaly phenotype (spleen/body weight) and levels of ATF4 (ATF4/ACTB) in splenocytes from <i>vavP-Bcl2;Sirt3</i><sup>+/+</sup> and <i>vavP-Bcl2;Sirt3</i><sup>−/−</sup> mice. ATF4 levels were quantified with densitometry results from Western blots. <b>G,</b> Western blot results show ATF4 levels from human DLBCL tumor samples or normal GC B cells from human tonsil. ACTB levels were used as loading control. <b>H,</b> GSEA shows the enrichment of ATF4 target genes in DLBCL tumors versus normal GC B cells. Gene expression data were from published microarray data (<a href="#bib53" target="_blank">53</a>). Error bars represent the mean ± SD of three or more replicates.</p> |
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