Figure 4 from Translational Activation of ATF4 through Mitochondrial Anaplerotic Metabolic Pathways Is Required for DLBCL Growth and Survival
<p>ATF4 protein level is decreased in <i>vavP-Bcl2;Sirt3</i><sup>−/−</sup> mice and associated with lymphoma progression. <b>A,</b> Western blot results show the protein levels of ATF4, LC3, EIF2A, ACTB, and SIRT3 in splenocytes from <i>vavP-Bcl2;Sirt3...
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2025
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| _version_ | 1849927640600805376 |
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| author | Meng Li (79487) |
| author2 | Matthew R. Teater (15129060) Jun Young Hong (6588914) Noel R. Park (15129063) Cihangir Duy (15112006) Hao Shen (195461) Ling Wang (56577) Zhengming Chen (385056) Leandro Cerchietti (15091552) Shawn M. Davidson (14911596) Hening Lin (1306563) Ari M. Melnick (15110132) |
| author2_role | author author author author author author author author author author author |
| author_facet | Meng Li (79487) Matthew R. Teater (15129060) Jun Young Hong (6588914) Noel R. Park (15129063) Cihangir Duy (15112006) Hao Shen (195461) Ling Wang (56577) Zhengming Chen (385056) Leandro Cerchietti (15091552) Shawn M. Davidson (14911596) Hening Lin (1306563) Ari M. Melnick (15110132) |
| author_role | author |
| dc.creator.none.fl_str_mv | Meng Li (79487) Matthew R. Teater (15129060) Jun Young Hong (6588914) Noel R. Park (15129063) Cihangir Duy (15112006) Hao Shen (195461) Ling Wang (56577) Zhengming Chen (385056) Leandro Cerchietti (15091552) Shawn M. Davidson (14911596) Hening Lin (1306563) Ari M. Melnick (15110132) |
| dc.date.none.fl_str_mv | 2025-11-24T22:01:41Z |
| dc.identifier.none.fl_str_mv | 10.1158/2643-3230.30698625 |
| dc.relation.none.fl_str_mv | https://figshare.com/articles/figure/Figure_4_from_Translational_Activation_of_ATF4_through_Mitochondrial_Anaplerotic_Metabolic_Pathways_Is_Required_for_DLBCL_Growth_and_Survival/30698625 |
| dc.rights.none.fl_str_mv | CC BY info:eu-repo/semantics/openAccess |
| dc.subject.none.fl_str_mv | Cancer Molecular and Cellular Biology Cellular Stress Responses Gene Regulation Posttranscriptional and translational control Hematological Cancers Lymphomas Metabolism |
| dc.title.none.fl_str_mv | Figure 4 from Translational Activation of ATF4 through Mitochondrial Anaplerotic Metabolic Pathways Is Required for DLBCL Growth and Survival |
| dc.type.none.fl_str_mv | Image Figure info:eu-repo/semantics/publishedVersion image |
| description | <p>ATF4 protein level is decreased in <i>vavP-Bcl2;Sirt3</i><sup>−/−</sup> mice and associated with lymphoma progression. <b>A,</b> Western blot results show the protein levels of ATF4, LC3, EIF2A, ACTB, and SIRT3 in splenocytes from <i>vavP-Bcl2;Sirt3</i><sup>+/+</sup> and <i>vavP-Bcl2;Sirt3</i><sup>−/−</sup> mice. The protein amounts were quantified with densitometry results. <b>B,</b> Summarized results of ATF4 protein level normalized to ACTB in splenocytes from <i>vavP-Bcl2;Sirt3</i><sup>+/+</sup> and <i>vavP-Bcl2;Sirt3</i><sup>−/−</sup> mice. The protein amounts were quantified with densitometry results from Western blots. <b>C,</b> Correlation between levels of autophagy (LC3II/LC3I) and ATF4 (ATF4/ACTB) in splenocytes from <i>vavP-Bcl2;Sirt3</i><sup>+/+</sup> and <i>vavP-Bcl2;Sirt3</i><sup>−/−</sup> mice. The data for correlation study were obtained with densitometry results from Western blots. <b>D,</b> Correlation between levels of phospho-EIF2A (p-EIF2A/EIF2A) and ATF4 (ATF4/ACTB) in splenocytes from <i>vavP-Bcl2;Sirt3</i><sup>+/+</sup> and <i>vavP-Bcl2;Sirt3</i><sup>−/−</sup> mice. The data for correlation study were obtained with densitometry results from Western blots. <b>E,</b> Summarized results of phospho-EIF2A level normalized to total EIF2A in splenocytes from <i>vavP-Bcl2;Sirt3</i><sup>+/+</sup> and <i>vavP-Bcl2;Sirt3</i><sup>−/−</sup> mice. The protein amounts were quantified with densitometry results from Western blots. <b>F,</b> Correlation between splenomegaly phenotype (spleen/body weight) and levels of ATF4 (ATF4/ACTB) in splenocytes from <i>vavP-Bcl2;Sirt3</i><sup>+/+</sup> and <i>vavP-Bcl2;Sirt3</i><sup>−/−</sup> mice. ATF4 levels were quantified with densitometry results from Western blots. <b>G,</b> Western blot results show ATF4 levels from human DLBCL tumor samples or normal GC B cells from human tonsil. ACTB levels were used as loading control. <b>H,</b> GSEA shows the enrichment of ATF4 target genes in DLBCL tumors versus normal GC B cells. Gene expression data were from published microarray data (<a href="#bib53" target="_blank">53</a>). Error bars represent the mean ± SD of three or more replicates.</p> |
| eu_rights_str_mv | openAccess |
| id | Manara_3ae091220bc57070718fdaacca1fdd90 |
| identifier_str_mv | 10.1158/2643-3230.30698625 |
| network_acronym_str | Manara |
| network_name_str | ManaraRepo |
| oai_identifier_str | oai:figshare.com:article/30698625 |
| publishDate | 2025 |
| repository.mail.fl_str_mv | |
| repository.name.fl_str_mv | |
| repository_id_str | |
| rights_invalid_str_mv | CC BY |
| spelling | Figure 4 from Translational Activation of ATF4 through Mitochondrial Anaplerotic Metabolic Pathways Is Required for DLBCL Growth and SurvivalMeng Li (79487)Matthew R. Teater (15129060)Jun Young Hong (6588914)Noel R. Park (15129063)Cihangir Duy (15112006)Hao Shen (195461)Ling Wang (56577)Zhengming Chen (385056)Leandro Cerchietti (15091552)Shawn M. Davidson (14911596)Hening Lin (1306563)Ari M. Melnick (15110132)CancerMolecular and Cellular BiologyCellular Stress ResponsesGene RegulationPosttranscriptional and translational controlHematological CancersLymphomasMetabolism<p>ATF4 protein level is decreased in <i>vavP-Bcl2;Sirt3</i><sup>−/−</sup> mice and associated with lymphoma progression. <b>A,</b> Western blot results show the protein levels of ATF4, LC3, EIF2A, ACTB, and SIRT3 in splenocytes from <i>vavP-Bcl2;Sirt3</i><sup>+/+</sup> and <i>vavP-Bcl2;Sirt3</i><sup>−/−</sup> mice. The protein amounts were quantified with densitometry results. <b>B,</b> Summarized results of ATF4 protein level normalized to ACTB in splenocytes from <i>vavP-Bcl2;Sirt3</i><sup>+/+</sup> and <i>vavP-Bcl2;Sirt3</i><sup>−/−</sup> mice. The protein amounts were quantified with densitometry results from Western blots. <b>C,</b> Correlation between levels of autophagy (LC3II/LC3I) and ATF4 (ATF4/ACTB) in splenocytes from <i>vavP-Bcl2;Sirt3</i><sup>+/+</sup> and <i>vavP-Bcl2;Sirt3</i><sup>−/−</sup> mice. The data for correlation study were obtained with densitometry results from Western blots. <b>D,</b> Correlation between levels of phospho-EIF2A (p-EIF2A/EIF2A) and ATF4 (ATF4/ACTB) in splenocytes from <i>vavP-Bcl2;Sirt3</i><sup>+/+</sup> and <i>vavP-Bcl2;Sirt3</i><sup>−/−</sup> mice. The data for correlation study were obtained with densitometry results from Western blots. <b>E,</b> Summarized results of phospho-EIF2A level normalized to total EIF2A in splenocytes from <i>vavP-Bcl2;Sirt3</i><sup>+/+</sup> and <i>vavP-Bcl2;Sirt3</i><sup>−/−</sup> mice. The protein amounts were quantified with densitometry results from Western blots. <b>F,</b> Correlation between splenomegaly phenotype (spleen/body weight) and levels of ATF4 (ATF4/ACTB) in splenocytes from <i>vavP-Bcl2;Sirt3</i><sup>+/+</sup> and <i>vavP-Bcl2;Sirt3</i><sup>−/−</sup> mice. ATF4 levels were quantified with densitometry results from Western blots. <b>G,</b> Western blot results show ATF4 levels from human DLBCL tumor samples or normal GC B cells from human tonsil. ACTB levels were used as loading control. <b>H,</b> GSEA shows the enrichment of ATF4 target genes in DLBCL tumors versus normal GC B cells. Gene expression data were from published microarray data (<a href="#bib53" target="_blank">53</a>). Error bars represent the mean ± SD of three or more replicates.</p>2025-11-24T22:01:41ZImageFigureinfo:eu-repo/semantics/publishedVersionimage10.1158/2643-3230.30698625https://figshare.com/articles/figure/Figure_4_from_Translational_Activation_of_ATF4_through_Mitochondrial_Anaplerotic_Metabolic_Pathways_Is_Required_for_DLBCL_Growth_and_Survival/30698625CC BYinfo:eu-repo/semantics/openAccessoai:figshare.com:article/306986252025-11-24T22:01:41Z |
| spellingShingle | Figure 4 from Translational Activation of ATF4 through Mitochondrial Anaplerotic Metabolic Pathways Is Required for DLBCL Growth and Survival Meng Li (79487) Cancer Molecular and Cellular Biology Cellular Stress Responses Gene Regulation Posttranscriptional and translational control Hematological Cancers Lymphomas Metabolism |
| status_str | publishedVersion |
| title | Figure 4 from Translational Activation of ATF4 through Mitochondrial Anaplerotic Metabolic Pathways Is Required for DLBCL Growth and Survival |
| title_full | Figure 4 from Translational Activation of ATF4 through Mitochondrial Anaplerotic Metabolic Pathways Is Required for DLBCL Growth and Survival |
| title_fullStr | Figure 4 from Translational Activation of ATF4 through Mitochondrial Anaplerotic Metabolic Pathways Is Required for DLBCL Growth and Survival |
| title_full_unstemmed | Figure 4 from Translational Activation of ATF4 through Mitochondrial Anaplerotic Metabolic Pathways Is Required for DLBCL Growth and Survival |
| title_short | Figure 4 from Translational Activation of ATF4 through Mitochondrial Anaplerotic Metabolic Pathways Is Required for DLBCL Growth and Survival |
| title_sort | Figure 4 from Translational Activation of ATF4 through Mitochondrial Anaplerotic Metabolic Pathways Is Required for DLBCL Growth and Survival |
| topic | Cancer Molecular and Cellular Biology Cellular Stress Responses Gene Regulation Posttranscriptional and translational control Hematological Cancers Lymphomas Metabolism |