Workflow for Nanostring-based miRNA biomarker assay.

<p>(A)The flowchart illustrates the overall experimental procedures. The workflow consists of RNA sample preparation steps(blue), Nanostring nCounter sample preparation steps(green), and an image analysis step to obtain raw count data(orange). (B) The flowchart presents the data analysis steps...

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Bibliographic Details
Main Author:	Josephine Y. Fang (19959341) (author)
Other Authors:	Saravanan Ayyadurai (222653) (author), Alyssa F. Pybus (18826651) (author), Hiroshi Sugimoto (1361628) (author), Mark G. Qian (2125579) (author)
Published:	2024
Subjects:	Medicine Cell Biology Immunology Cancer Space Science Environmental Sciences not elsewhere classified Biological Sciences not elsewhere classified Chemical Sciences not elsewhere classified Information Systems not elsewhere classified improved patient outcomes highly differential mirnas enzyme replacement therapy differential mirnas may rna input concentration mirna expression indicated isolation method employed identify mirna candidates highly differential mirna fabry disease serum fabry disease patients tm </ sup qiagen mirneasy kit div >< p clinical serum samples angiogenesis pathways suggests identify mirna biomarkers fabry disease using fabry disease angiogenesis pathways rna extraction mirna signatures isolation methods disease progression disease groups reliable biomarkers potential biomarkers performed using manually using validation studies ultimately leading reproducible results raw count magmax mirvana healthy subjects gene mutations galactosidase α findings lay ensuring reliable enrichment analysis early diagnosis distinct depending diagnostic potential detection range consistent association
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Workflow for Nanostring-based miRNA biomarker assay.

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