YqhG is required for systemic virulence but not for <i>in vitro</i> growth.
<p>(A) Growth curves of MRSA wild-type (WT), Δ<i>yqhG</i> mutant, and complemented strain (Δ<i>yqhG</i>-C) cultured in TSB at 37 °C with shaking. (B) Kaplan–Meier survival analysis of BALB/c mice (n = 10 per group) following intravenous infection with 2 × 10⁷ CFU of eac...
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2025
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| _version_ | 1849927628608241664 |
|---|---|
| author | Jianhua Liao (1393228) |
| author2 | Jun Cheng (194158) Baoqing Liu (1508821) Yuzhi Shao (22683349) Chunyan Meng (5993228) |
| author2_role | author author author author |
| author_facet | Jianhua Liao (1393228) Jun Cheng (194158) Baoqing Liu (1508821) Yuzhi Shao (22683349) Chunyan Meng (5993228) |
| author_role | author |
| dc.creator.none.fl_str_mv | Jianhua Liao (1393228) Jun Cheng (194158) Baoqing Liu (1508821) Yuzhi Shao (22683349) Chunyan Meng (5993228) |
| dc.date.none.fl_str_mv | 2025-11-25T18:28:02Z |
| dc.identifier.none.fl_str_mv | 10.1371/journal.pone.0337292.g001 |
| dc.relation.none.fl_str_mv | https://figshare.com/articles/figure/YqhG_is_required_for_systemic_virulence_but_not_for_i_in_vitro_i_growth_/30713749 |
| dc.rights.none.fl_str_mv | CC BY 4.0 info:eu-repo/semantics/openAccess |
| dc.subject.none.fl_str_mv | Medicine Microbiology Cell Biology Biotechnology Evolutionary Biology Ecology Science Policy Infectious Diseases Virology Environmental Sciences not elsewhere classified Biological Sciences not elsewhere classified therapeutic strategies aimed novel therapeutic approaches murine infection model div >< p defense mechanisms employed conserved gene encoding compromising membrane stability affect biofilm formation staphylococcus aureus </ reduced bacterial burden improved host survival maintaining mrsa ’ oxidative stress resistance yqhg </ withstand host reduced motility oxidative stress increasing resistance stress adaptation potential target periplasmic protein mrsa virulence mrsa pathogenesis key determinant infected organs increased sensitivity imposed stresses growing prevalence findings highlight existing antibiotics critical need attenuates virulence |
| dc.title.none.fl_str_mv | YqhG is required for systemic virulence but not for <i>in vitro</i> growth. |
| dc.type.none.fl_str_mv | Image Figure info:eu-repo/semantics/publishedVersion image |
| description | <p>(A) Growth curves of MRSA wild-type (WT), Δ<i>yqhG</i> mutant, and complemented strain (Δ<i>yqhG</i>-C) cultured in TSB at 37 °C with shaking. (B) Kaplan–Meier survival analysis of BALB/c mice (n = 10 per group) following intravenous infection with 2 × 10⁷ CFU of each strain. (C-F) Bacterial burden in the heart (C), kidney (D), liver (E) and spleen (F) at 24 h post-infection. CFU were enumerated from homogenized tissues. Data represent at least three independent experiments. Statistical analyses were performed using one-way ANOVA (C-F) and log-rank test (B). *<i>P</i> < 0.05, **<i>P</i> < 0.01, ***<i>P</i> < 0.001.</p> |
| eu_rights_str_mv | openAccess |
| id | Manara_3eca6ed85c70f5db15d0a8a109cdc63f |
| identifier_str_mv | 10.1371/journal.pone.0337292.g001 |
| network_acronym_str | Manara |
| network_name_str | ManaraRepo |
| oai_identifier_str | oai:figshare.com:article/30713749 |
| publishDate | 2025 |
| repository.mail.fl_str_mv | |
| repository.name.fl_str_mv | |
| repository_id_str | |
| rights_invalid_str_mv | CC BY 4.0 |
| spelling | YqhG is required for systemic virulence but not for <i>in vitro</i> growth.Jianhua Liao (1393228)Jun Cheng (194158)Baoqing Liu (1508821)Yuzhi Shao (22683349)Chunyan Meng (5993228)MedicineMicrobiologyCell BiologyBiotechnologyEvolutionary BiologyEcologyScience PolicyInfectious DiseasesVirologyEnvironmental Sciences not elsewhere classifiedBiological Sciences not elsewhere classifiedtherapeutic strategies aimednovel therapeutic approachesmurine infection modeldiv >< pdefense mechanisms employedconserved gene encodingcompromising membrane stabilityaffect biofilm formationstaphylococcus aureus </reduced bacterial burdenimproved host survivalmaintaining mrsa ’oxidative stress resistanceyqhg </withstand hostreduced motilityoxidative stressincreasing resistancestress adaptationpotential targetperiplasmic proteinmrsa virulencemrsa pathogenesiskey determinantinfected organsincreased sensitivityimposed stressesgrowing prevalencefindings highlightexisting antibioticscritical needattenuates virulence<p>(A) Growth curves of MRSA wild-type (WT), Δ<i>yqhG</i> mutant, and complemented strain (Δ<i>yqhG</i>-C) cultured in TSB at 37 °C with shaking. (B) Kaplan–Meier survival analysis of BALB/c mice (n = 10 per group) following intravenous infection with 2 × 10⁷ CFU of each strain. (C-F) Bacterial burden in the heart (C), kidney (D), liver (E) and spleen (F) at 24 h post-infection. CFU were enumerated from homogenized tissues. Data represent at least three independent experiments. Statistical analyses were performed using one-way ANOVA (C-F) and log-rank test (B). *<i>P</i> < 0.05, **<i>P</i> < 0.01, ***<i>P</i> < 0.001.</p>2025-11-25T18:28:02ZImageFigureinfo:eu-repo/semantics/publishedVersionimage10.1371/journal.pone.0337292.g001https://figshare.com/articles/figure/YqhG_is_required_for_systemic_virulence_but_not_for_i_in_vitro_i_growth_/30713749CC BY 4.0info:eu-repo/semantics/openAccessoai:figshare.com:article/307137492025-11-25T18:28:02Z |
| spellingShingle | YqhG is required for systemic virulence but not for <i>in vitro</i> growth. Jianhua Liao (1393228) Medicine Microbiology Cell Biology Biotechnology Evolutionary Biology Ecology Science Policy Infectious Diseases Virology Environmental Sciences not elsewhere classified Biological Sciences not elsewhere classified therapeutic strategies aimed novel therapeutic approaches murine infection model div >< p defense mechanisms employed conserved gene encoding compromising membrane stability affect biofilm formation staphylococcus aureus </ reduced bacterial burden improved host survival maintaining mrsa ’ oxidative stress resistance yqhg </ withstand host reduced motility oxidative stress increasing resistance stress adaptation potential target periplasmic protein mrsa virulence mrsa pathogenesis key determinant infected organs increased sensitivity imposed stresses growing prevalence findings highlight existing antibiotics critical need attenuates virulence |
| status_str | publishedVersion |
| title | YqhG is required for systemic virulence but not for <i>in vitro</i> growth. |
| title_full | YqhG is required for systemic virulence but not for <i>in vitro</i> growth. |
| title_fullStr | YqhG is required for systemic virulence but not for <i>in vitro</i> growth. |
| title_full_unstemmed | YqhG is required for systemic virulence but not for <i>in vitro</i> growth. |
| title_short | YqhG is required for systemic virulence but not for <i>in vitro</i> growth. |
| title_sort | YqhG is required for systemic virulence but not for <i>in vitro</i> growth. |
| topic | Medicine Microbiology Cell Biology Biotechnology Evolutionary Biology Ecology Science Policy Infectious Diseases Virology Environmental Sciences not elsewhere classified Biological Sciences not elsewhere classified therapeutic strategies aimed novel therapeutic approaches murine infection model div >< p defense mechanisms employed conserved gene encoding compromising membrane stability affect biofilm formation staphylococcus aureus </ reduced bacterial burden improved host survival maintaining mrsa ’ oxidative stress resistance yqhg </ withstand host reduced motility oxidative stress increasing resistance stress adaptation potential target periplasmic protein mrsa virulence mrsa pathogenesis key determinant infected organs increased sensitivity imposed stresses growing prevalence findings highlight existing antibiotics critical need attenuates virulence |