YqhG is required for systemic virulence but not for <i>in vitro</i> growth.

YqhG is required for systemic virulence but not for <i>in vitro</i> growth.

<p>(A) Growth curves of MRSA wild-type (WT), Δ<i>yqhG</i> mutant, and complemented strain (Δ<i>yqhG</i>-C) cultured in TSB at 37 °C with shaking. (B) Kaplan–Meier survival analysis of BALB/c mice (n = 10 per group) following intravenous infection with 2 × 10⁷ CFU of eac...

Volledige beschrijving

Bewaard in:

Bibliografische gegevens
Hoofdauteur:	Jianhua Liao (1393228) (author)
Andere auteurs:	Jun Cheng (194158) (author), Baoqing Liu (1508821) (author), Yuzhi Shao (22683349) (author), Chunyan Meng (5993228) (author)
Gepubliceerd in:	2025
Onderwerpen:	Medicine Microbiology Cell Biology Biotechnology Evolutionary Biology Ecology Science Policy Infectious Diseases Virology Environmental Sciences not elsewhere classified Biological Sciences not elsewhere classified therapeutic strategies aimed novel therapeutic approaches murine infection model div >< p defense mechanisms employed conserved gene encoding compromising membrane stability affect biofilm formation staphylococcus aureus </ reduced bacterial burden improved host survival maintaining mrsa ’ oxidative stress resistance yqhg </ withstand host reduced motility oxidative stress increasing resistance stress adaptation potential target periplasmic protein mrsa virulence mrsa pathogenesis key determinant infected organs increased sensitivity imposed stresses growing prevalence findings highlight existing antibiotics critical need attenuates virulence
Tags:	Voeg label toe Geen labels, Wees de eerste die dit record labelt!

Gelijkaardige items

Optical density measurements of MRSA wild-type, Δ<i>yqhG</i>, and complemented strains during <i>in vitro</i> growth.
door: Jianhua Liao (1393228)
Gepubliceerd in: (2025)

Surface motility of the <i>yqhG</i> mutant.
door: Jianhua Liao (1393228)
Gepubliceerd in: (2025)

Membrane integrity in the <i>yqhG</i> mutant.
door: Jianhua Liao (1393228)
Gepubliceerd in: (2025)

Oxidative stress resistance of the <i>yqhG</i> mutant.
door: Jianhua Liao (1393228)
Gepubliceerd in: (2025)

Kidney bacterial burden in mice infected with MRSA wild-type, Δ<i>yqhG</i>, and complemented strains.
door: Jianhua Liao (1393228)
Gepubliceerd in: (2025)

Heart bacterial burden in mice infected with MRSA wild-type, Δ<i>yqhG</i>, and complemented strains.
door: Jianhua Liao (1393228)
Gepubliceerd in: (2025)

Survival data of BALB/c mice infected with MRSA wild-type, Δ<i>yqhG</i>, and complemented strains.
door: Jianhua Liao (1393228)
Gepubliceerd in: (2025)

Crystal violet quantification of biofilm formation in MRSA wild-type, ΔyqhG, and complemented strains.
door: Jianhua Liao (1393228)
Gepubliceerd in: (2025)

Spleen bacterial burden in mice infected with MRSA wild-type, ΔyqhG, and complemented strains.
door: Jianhua Liao (1393228)
Gepubliceerd in: (2025)

Liver bacterial burden in mice infected with MRSA wild-type, ΔyqhG, and complemented strains.
door: Jianhua Liao (1393228)
Gepubliceerd in: (2025)

Amount of PSMs (μg/1mL culture) in MRSA wild-type, ΔyqhG, and complemented strains.
door: Jianhua Liao (1393228)
Gepubliceerd in: (2025)

Quantification of cell-associated PSMs in wild-type, Δ<i>yqhG</i>, and complemented strains. Cell-associated PSMs were extracted from bacterial pellets using 6 M guanidine hydrochloride and quantified by reversed-phase HPLC. The Δ<i>yqhG</i> mutant exhibited a significant reduction in surface-bound PSMs compared with the wild-type strain, whereas complementation restored PSM levels to near wild-type values. Data represent mean ± s.e.m. from three independent experiments. Statistical analysis was performed using one-way ANOVA; *<i>P</i> < 0.05, ns, not significant.
door: Jianhua Liao (1393228)
Gepubliceerd in: (2025)

Primers Used in This Study.
door: Jianhua Liao (1393228)
Gepubliceerd in: (2025)

Bacterial Strains and Plasmids Used in This Study.
door: Jianhua Liao (1393228)
Gepubliceerd in: (2025)

Biofilm formation in MRSA strains.
door: Jianhua Liao (1393228)
Gepubliceerd in: (2025)

Viable CFU counts following hydrogen peroxide exposure.
door: Jianhua Liao (1393228)
Gepubliceerd in: (2025)

Colony diameter measurements from soft agar motility assays.
door: Jianhua Liao (1393228)
Gepubliceerd in: (2025)

SYTOX Green fluorescence measurements for membrane permeability assessment.
door: Jianhua Liao (1393228)
Gepubliceerd in: (2025)

Table 1_Hard to jump: host shifts appear unlikely in a T4-like phage evolved in the lab.xlsx
door: Yu Ning (588573)
Gepubliceerd in: (2025)

Supplementary file 1_Hard to jump: host shifts appear unlikely in a T4-like phage evolved in the lab.docx
door: Yu Ning (588573)
Gepubliceerd in: (2025)

Protocatechuic Acid alleviates D-gal-induced renal senescence and injury in mice by regulating taurine metabolism
door: Songhao Tian (22609826)
Gepubliceerd in: (2025)

Table 1_Melatonin mitigates cadmium-induced oxidative damage and modulates polysaccharide biosynthesis in Bletilla striata.xlsx
door: Deqiong Niu (19691873)
Gepubliceerd in: (2025)

Data Sheet 1_Melatonin mitigates cadmium-induced oxidative damage and modulates polysaccharide biosynthesis in Bletilla striata.docx
door: Deqiong Niu (19691873)
Gepubliceerd in: (2025)

Potent phenolics with antimigratory and anti-MRSA powers from fungus associated with <i>Saussurea involucrata</i>
door: Wei-hua Yuan (22683169)
Gepubliceerd in: (2025)

Effects of <i>T. gondii</i> infection on rat gut microbiota diversity.
door: Ji-Xin Zhao (22056386)
Gepubliceerd in: (2025)

Untargeted serum metabolomics analysis before and after <i>T. gondii</i> infection.
door: Ji-Xin Zhao (22056386)
Gepubliceerd in: (2025)

Functional analysis of the intestinal microbial community following <i>T. gondii</i> infection.
door: Ji-Xin Zhao (22056386)
Gepubliceerd in: (2025)

Composition of intestinal microbial communities in the large intestine of rats before and after <i>T. gondii</i> infection.
door: Ji-Xin Zhao (22056386)
Gepubliceerd in: (2025)

Composition of intestinal microbial communities in the small intestine of rats before and after <i>T. gondii</i> infection.
door: Ji-Xin Zhao (22056386)
Gepubliceerd in: (2025)

Differential metabolite results, listing metabolites with significant changes across treatment groups, including fold changes, and <i>p</i>-values.
door: Ji-Xin Zhao (22056386)
Gepubliceerd in: (2025)

Correlation network between intestinal bacterial species and differential serum metabolites.
door: Ji-Xin Zhao (22056386)
Gepubliceerd in: (2025)

KEGG pathway enrichment analysis of differential serum metabolites.
door: Ji-Xin Zhao (22056386)
Gepubliceerd in: (2025)

Spearman correlation analysis between gut microbial taxa and circulating metabolites, detailing correlation coefficients, significance values, and taxon–metabolite pairings indicative of microbiome–host metabolic interactions.
door: Ji-Xin Zhao (22056386)
Gepubliceerd in: (2025)

Pathway enrichment analysis of differential metabolites, highlighting significantly impacted metabolic pathways and associated enrichment statistics based on KEGG or other pathway databases.
door: Ji-Xin Zhao (22056386)
Gepubliceerd in: (2025)

Orthogonal partial least squares-discriminant analysis (OPLS-DA) of serum metabolomics data.
door: Ji-Xin Zhao (22056386)
Gepubliceerd in: (2025)

Quality control assessment of serum metabolomics data.
door: Ji-Xin Zhao (22056386)
Gepubliceerd in: (2025)

Heatmap of enriched KEGG pathways across samples, illustrating variations in pathway abundance.
door: Ji-Xin Zhao (22056386)
Gepubliceerd in: (2025)

Graphical abstract.
door: Ji-Xin Zhao (22056386)
Gepubliceerd in: (2025)

The U sequence method.
door: Pierre Angelvy (22683450)
Gepubliceerd in: (2025)

Provisional study schedule of MUSIBOT.
door: Pierre Angelvy (22683450)
Gepubliceerd in: (2025)

Cannot write session to /tmp/vufind_sessions/sess_8v7pkj1vgc4rglgclmin9e8r67