Filamin A phosphorylation is essential for ORF45-induced detachment and migration but not for virion production.
<p>A-D. HEK293 cells were transfected with Filamin A WT or S2152A mutated constructs and then infected with empty vector or ORF45-expressing lentiviruses at 8 h after transfection. Twenty-four hours later, the cell morphology was observed by inverted microscopy, and representative images are s...
محفوظ في:
| المؤلف الرئيسي: | |
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| مؤلفون آخرون: | , , , , |
| منشور في: |
2025
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| الموضوعات: | |
| الوسوم: |
إضافة وسم
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| الملخص: | <p>A-D. HEK293 cells were transfected with Filamin A WT or S2152A mutated constructs and then infected with empty vector or ORF45-expressing lentiviruses at 8 h after transfection. Twenty-four hours later, the cell morphology was observed by inverted microscopy, and representative images are shown (A). Thirty-six hours after infection, the cells were subjected to analysis for relative adhesion (B), gene expression by western blots (C), and cell migration in the wound healing scratch assay (D). E. Bac16-harboring 239T cells in 6-well plates were transfected with 1.6 μg of Filamin A WT or S2152A construct for 24 h, and then, the cells were induced with 20 ng/ml TPA and 0.3 mm NaB for lytic replication. The supernatants were collected at different time points, and then, the yield of progeny viruses was detected with quantitative PCR analysis.</p> |
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