Filamin A phosphorylation is essential for ORF45-induced detachment and migration but not for virion production.
<p>A-D. HEK293 cells were transfected with Filamin A WT or S2152A mutated constructs and then infected with empty vector or ORF45-expressing lentiviruses at 8 h after transfection. Twenty-four hours later, the cell morphology was observed by inverted microscopy, and representative images are s...
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| _version_ | 1849927641318031360 |
|---|---|
| author | Xiaojuan Li (351842) |
| author2 | Li Quan (177024) Rihong Zhou (22676690) Xiangpeng Liu (17898881) Ronit Sarid (186795) Ersheng Kuang (129028) |
| author2_role | author author author author author |
| author_facet | Xiaojuan Li (351842) Li Quan (177024) Rihong Zhou (22676690) Xiangpeng Liu (17898881) Ronit Sarid (186795) Ersheng Kuang (129028) |
| author_role | author |
| dc.creator.none.fl_str_mv | Xiaojuan Li (351842) Li Quan (177024) Rihong Zhou (22676690) Xiangpeng Liu (17898881) Ronit Sarid (186795) Ersheng Kuang (129028) |
| dc.date.none.fl_str_mv | 2025-11-24T18:34:25Z |
| dc.identifier.none.fl_str_mv | 10.1371/journal.ppat.1013737.g004 |
| dc.relation.none.fl_str_mv | https://figshare.com/articles/figure/Filamin_A_phosphorylation_is_essential_for_ORF45-induced_detachment_and_migration_but_not_for_virion_production_/30697703 |
| dc.rights.none.fl_str_mv | CC BY 4.0 info:eu-repo/semantics/openAccess |
| dc.subject.none.fl_str_mv | Microbiology Cell Biology Molecular Biology Biotechnology Immunology Developmental Biology Cancer Infectious Diseases Virology s2152a knockin abolish primary effusion lymphoma multicentric castleman disease kaposi &# 8217 mediated viral infection infected adherent cells differentiated b cells sequential cell detachment mediated kshv transmission kshv lytic replication adherent cells lytic replication viral transmission kshv transmission cell detachment b lymphocytes lytic cycle cell transmission taken together results demonstrated radically support natural donors key role key regulator etiological agent dependent manner cell contact cell adhesion associated herpesvirus |
| dc.title.none.fl_str_mv | Filamin A phosphorylation is essential for ORF45-induced detachment and migration but not for virion production. |
| dc.type.none.fl_str_mv | Image Figure info:eu-repo/semantics/publishedVersion image |
| description | <p>A-D. HEK293 cells were transfected with Filamin A WT or S2152A mutated constructs and then infected with empty vector or ORF45-expressing lentiviruses at 8 h after transfection. Twenty-four hours later, the cell morphology was observed by inverted microscopy, and representative images are shown (A). Thirty-six hours after infection, the cells were subjected to analysis for relative adhesion (B), gene expression by western blots (C), and cell migration in the wound healing scratch assay (D). E. Bac16-harboring 239T cells in 6-well plates were transfected with 1.6 μg of Filamin A WT or S2152A construct for 24 h, and then, the cells were induced with 20 ng/ml TPA and 0.3 mm NaB for lytic replication. The supernatants were collected at different time points, and then, the yield of progeny viruses was detected with quantitative PCR analysis.</p> |
| eu_rights_str_mv | openAccess |
| id | Manara_3efb57c03e52065cd7e26ff72ef7285f |
| identifier_str_mv | 10.1371/journal.ppat.1013737.g004 |
| network_acronym_str | Manara |
| network_name_str | ManaraRepo |
| oai_identifier_str | oai:figshare.com:article/30697703 |
| publishDate | 2025 |
| repository.mail.fl_str_mv | |
| repository.name.fl_str_mv | |
| repository_id_str | |
| rights_invalid_str_mv | CC BY 4.0 |
| spelling | Filamin A phosphorylation is essential for ORF45-induced detachment and migration but not for virion production.Xiaojuan Li (351842)Li Quan (177024)Rihong Zhou (22676690)Xiangpeng Liu (17898881)Ronit Sarid (186795)Ersheng Kuang (129028)MicrobiologyCell BiologyMolecular BiologyBiotechnologyImmunologyDevelopmental BiologyCancerInfectious DiseasesVirologys2152a knockin abolishprimary effusion lymphomamulticentric castleman diseasekaposi &# 8217mediated viral infectioninfected adherent cellsdifferentiated b cellssequential cell detachmentmediated kshv transmissionkshv lytic replicationadherent cellslytic replicationviral transmissionkshv transmissioncell detachmentb lymphocyteslytic cyclecell transmissiontaken togetherresults demonstratedradically supportnatural donorskey rolekey regulatoretiological agentdependent mannercell contactcell adhesionassociated herpesvirus<p>A-D. HEK293 cells were transfected with Filamin A WT or S2152A mutated constructs and then infected with empty vector or ORF45-expressing lentiviruses at 8 h after transfection. Twenty-four hours later, the cell morphology was observed by inverted microscopy, and representative images are shown (A). Thirty-six hours after infection, the cells were subjected to analysis for relative adhesion (B), gene expression by western blots (C), and cell migration in the wound healing scratch assay (D). E. Bac16-harboring 239T cells in 6-well plates were transfected with 1.6 μg of Filamin A WT or S2152A construct for 24 h, and then, the cells were induced with 20 ng/ml TPA and 0.3 mm NaB for lytic replication. The supernatants were collected at different time points, and then, the yield of progeny viruses was detected with quantitative PCR analysis.</p>2025-11-24T18:34:25ZImageFigureinfo:eu-repo/semantics/publishedVersionimage10.1371/journal.ppat.1013737.g004https://figshare.com/articles/figure/Filamin_A_phosphorylation_is_essential_for_ORF45-induced_detachment_and_migration_but_not_for_virion_production_/30697703CC BY 4.0info:eu-repo/semantics/openAccessoai:figshare.com:article/306977032025-11-24T18:34:25Z |
| spellingShingle | Filamin A phosphorylation is essential for ORF45-induced detachment and migration but not for virion production. Xiaojuan Li (351842) Microbiology Cell Biology Molecular Biology Biotechnology Immunology Developmental Biology Cancer Infectious Diseases Virology s2152a knockin abolish primary effusion lymphoma multicentric castleman disease kaposi &# 8217 mediated viral infection infected adherent cells differentiated b cells sequential cell detachment mediated kshv transmission kshv lytic replication adherent cells lytic replication viral transmission kshv transmission cell detachment b lymphocytes lytic cycle cell transmission taken together results demonstrated radically support natural donors key role key regulator etiological agent dependent manner cell contact cell adhesion associated herpesvirus |
| status_str | publishedVersion |
| title | Filamin A phosphorylation is essential for ORF45-induced detachment and migration but not for virion production. |
| title_full | Filamin A phosphorylation is essential for ORF45-induced detachment and migration but not for virion production. |
| title_fullStr | Filamin A phosphorylation is essential for ORF45-induced detachment and migration but not for virion production. |
| title_full_unstemmed | Filamin A phosphorylation is essential for ORF45-induced detachment and migration but not for virion production. |
| title_short | Filamin A phosphorylation is essential for ORF45-induced detachment and migration but not for virion production. |
| title_sort | Filamin A phosphorylation is essential for ORF45-induced detachment and migration but not for virion production. |
| topic | Microbiology Cell Biology Molecular Biology Biotechnology Immunology Developmental Biology Cancer Infectious Diseases Virology s2152a knockin abolish primary effusion lymphoma multicentric castleman disease kaposi &# 8217 mediated viral infection infected adherent cells differentiated b cells sequential cell detachment mediated kshv transmission kshv lytic replication adherent cells lytic replication viral transmission kshv transmission cell detachment b lymphocytes lytic cycle cell transmission taken together results demonstrated radically support natural donors key role key regulator etiological agent dependent manner cell contact cell adhesion associated herpesvirus |