Figure 3 from Exploiting Natural Killer Cell Engagers to Control Pediatric B-cell Precursor Acute Lymphoblastic Leukemia

Figure 3 from Exploiting Natural Killer Cell Engagers to Control Pediatric B-cell Precursor Acute Lymphoblastic Leukemia

<p>Effect of NKCEs targeting CD19 or CD20 on primary BCP-ALL cells. <b>A,</b> Phenotypic characterization via flow cytometry of four BCP-ALL primary leukemia samples, using CD45-APC-Vio770, CD19-PE-Cy7, and CD20-V450. <b>B,</b> 7AAD/AnnV staining of cells from sample AL...

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Библиографические подробности
Главный автор: Natalia Colomar-Carando (14611340) (author)
Другие авторы: Laurent Gauthier (15129623) (author), Pietro Merli (15129626) (author), Fabrizio Loiacono (5306981) (author), Paolo Canevali (332990) (author), Michela Falco (368897) (author), Federica Galaverna (8825531) (author), Benjamin Rossi (15129629) (author), Frédéric Bosco (15129632) (author), Mélody Caratini (15129635) (author), Maria Cristina Mingari (8409327) (author), Franco Locatelli (15127797) (author), Eric Vivier (48284) (author), Raffaella Meazza (7179467) (author), Daniela Pende (15129638) (author)
Опубликовано: 2025
Предметы:
Cancer
Immuno-oncology
Hematological Cancers
Leukemias
Immunology
NK cells
Immunotherapy
Stem cell transplantation
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Итог:<p>Effect of NKCEs targeting CD19 or CD20 on primary BCP-ALL cells. <b>A,</b> Phenotypic characterization via flow cytometry of four BCP-ALL primary leukemia samples, using CD45-APC-Vio770, CD19-PE-Cy7, and CD20-V450. <b>B,</b> 7AAD/AnnV staining of cells from sample ALL#06 cultured either alone (only ALL#06) or with resting NK cells from a representative healthy donor and the indicated NKCEs (10<sup>0</sup> μg/mL). <b>C,</b> CD107a expression of NK cells cultured either alone or with cells from ALL#06 in the presence of IC-NKp46-NKCE, CD19-NKp46-NKCE, or CD20-NKp46-NKCE (10<sup>0</sup> μg/mL). A representative experiment is shown. Numbers indicate the percentage of cells in each quadrant. <b>D,</b> Percent of specific lysis of CD19<sup>+</sup> leukemia blasts (left) and CD107a degranulation (right) of resting NK cells from healthy donors (<i>n</i> = 3–6) upon coculture with primary leukemia blasts and NKCEs at 10<sup>0</sup> μg/mL as indicated. Data obtained with target cells containing leukemia blasts <85% (ALL#02 and ALL#07) or >85% (ALL#04 and ALL#06) were pooled. Results from 2–5 independent experiments are reported. The incubation time for all the tests was 4 hours. Bar show mean ± SEM. Statistical significance: *, <i>P</i> ≤ 0.05; **, <i>P</i> ≤ 0.01; ***, <i>P</i> ≤ 0.001. Mann–Whitney test was used to calculate statistical differences.</p>

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