Figure 3 from Exploiting Natural Killer Cell Engagers to Control Pediatric B-cell Precursor Acute Lymphoblastic Leukemia
<p>Effect of NKCEs targeting CD19 or CD20 on primary BCP-ALL cells. <b>A,</b> Phenotypic characterization via flow cytometry of four BCP-ALL primary leukemia samples, using CD45-APC-Vio770, CD19-PE-Cy7, and CD20-V450. <b>B,</b> 7AAD/AnnV staining of cells from sample AL...
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2025
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| 總結: | <p>Effect of NKCEs targeting CD19 or CD20 on primary BCP-ALL cells. <b>A,</b> Phenotypic characterization via flow cytometry of four BCP-ALL primary leukemia samples, using CD45-APC-Vio770, CD19-PE-Cy7, and CD20-V450. <b>B,</b> 7AAD/AnnV staining of cells from sample ALL#06 cultured either alone (only ALL#06) or with resting NK cells from a representative healthy donor and the indicated NKCEs (10<sup>0</sup> μg/mL). <b>C,</b> CD107a expression of NK cells cultured either alone or with cells from ALL#06 in the presence of IC-NKp46-NKCE, CD19-NKp46-NKCE, or CD20-NKp46-NKCE (10<sup>0</sup> μg/mL). A representative experiment is shown. Numbers indicate the percentage of cells in each quadrant. <b>D,</b> Percent of specific lysis of CD19<sup>+</sup> leukemia blasts (left) and CD107a degranulation (right) of resting NK cells from healthy donors (<i>n</i> = 3–6) upon coculture with primary leukemia blasts and NKCEs at 10<sup>0</sup> μg/mL as indicated. Data obtained with target cells containing leukemia blasts <85% (ALL#02 and ALL#07) or >85% (ALL#04 and ALL#06) were pooled. Results from 2–5 independent experiments are reported. The incubation time for all the tests was 4 hours. Bar show mean ± SEM. Statistical significance: *, <i>P</i> ≤ 0.05; **, <i>P</i> ≤ 0.01; ***, <i>P</i> ≤ 0.001. Mann–Whitney test was used to calculate statistical differences.</p> |
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