Differential lipid species analysis in siRNA-transfected human subcutaneous adipocytes.
<p>(A) Partial Least Squares Discriminant Analysis (PLS-DA) of lipids in the siRNA-transfected human subcutaneous adipocytes. (B) Dendrogram showing the relationship between lipid species in the siRNA-transfected human subcutaneous adipocytes using Euclidean distances and Ward’s clustering. (C...
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2025
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| Summary: | <p>(A) Partial Least Squares Discriminant Analysis (PLS-DA) of lipids in the siRNA-transfected human subcutaneous adipocytes. (B) Dendrogram showing the relationship between lipid species in the siRNA-transfected human subcutaneous adipocytes using Euclidean distances and Ward’s clustering. (C) Hierarchical clustering heatmap analysis in the siRNA-transfected human subcutaneous adipocytes was performed using Ward’s method with the Euclidean distance. Heatmaps show the top 96 lipid species that differed in the siRNA-transfected human subcutaneous adipocytes. The number at the bottom indicates the replicate number. The color scale indicates the number of standard deviations from the overall average of the lipid species, with areas in dark red indicating a higher amount of the lipid species, whereas areas in dark blue indicate a lower amount of the lipid species. The red, green, and blue colors represent cells transfected with the siRNA-negative control, siRNA-<i>GNB1</i>, and siRNA-<i>SCARB2</i>, respectively. (D) Scatterplots of <i>P</i>-values were obtained using one-way analysis of variance (ANOVA) of the 366 lipid species in the siRNA-transfected human subcutaneous adipocytes. The intensity of the red dots corresponds to the –log<sub>10</sub> transformed <i>P</i>-values obtained using one-way ANOVA (–log<sub>10</sub> (raw <i>P</i>-value)). After FDR correction of the ANOVA results, Fisher’s LSD post hoc tests were performed on significant lipids to determine specific group differences. Both figures were generated using MetaboAnalyst 6.0. Abbreviations: siRNA-nc, siRNA-negative control; siRNA-G, siRNA-G protein subunit beta 1 (<i>GNB1</i>); siRNA-S, siRNA-scavenger receptor class B member 2 (<i>SCARB2</i>).</p> |
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