Differential lipid species analysis in siRNA-transfected human subcutaneous adipocytes.
<p>(A) Partial Least Squares Discriminant Analysis (PLS-DA) of lipids in the siRNA-transfected human subcutaneous adipocytes. (B) Dendrogram showing the relationship between lipid species in the siRNA-transfected human subcutaneous adipocytes using Euclidean distances and Ward’s clustering. (C...
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2025
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| _version_ | 1851565143061692416 |
|---|---|
| author | Takuya Kitamoto (11721639) |
| author2 | Aya Kitamoto (256033) |
| author2_role | author |
| author_facet | Takuya Kitamoto (11721639) Aya Kitamoto (256033) |
| author_role | author |
| dc.creator.none.fl_str_mv | Takuya Kitamoto (11721639) Aya Kitamoto (256033) |
| dc.date.none.fl_str_mv | 2025-03-24T17:22:35Z |
| dc.identifier.none.fl_str_mv | 10.1371/journal.pone.0319163.g005 |
| dc.relation.none.fl_str_mv | https://figshare.com/articles/figure/Differential_lipid_species_analysis_in_siRNA-transfected_human_subcutaneous_adipocytes_/28653622 |
| dc.rights.none.fl_str_mv | CC BY 4.0 info:eu-repo/semantics/openAccess |
| dc.subject.none.fl_str_mv | Biochemistry Cell Biology Genetics Molecular Biology Physiology Environmental Sciences not elsewhere classified Biological Sciences not elsewhere classified Chemical Sciences not elsewhere classified wide association studies utilized rna interference molecular mechanisms linking human subcutaneous adipocytes div >< p including genetic predispositions lipid droplet accumulation adipocyte lipid storage knockdown also led although many obesity adipocyte fat accumulation >) — involved fat accumulation lipid metabolism adipocyte function proteins involved various factors upregulated following significant alterations scarb2 </ providing insights lipidomic analysis knockdown resulted integrative proteomic including plpp1 gwas data gwas ), gnb1 </ findings highlight cholesterol esters |
| dc.title.none.fl_str_mv | Differential lipid species analysis in siRNA-transfected human subcutaneous adipocytes. |
| dc.type.none.fl_str_mv | Image Figure info:eu-repo/semantics/publishedVersion image |
| description | <p>(A) Partial Least Squares Discriminant Analysis (PLS-DA) of lipids in the siRNA-transfected human subcutaneous adipocytes. (B) Dendrogram showing the relationship between lipid species in the siRNA-transfected human subcutaneous adipocytes using Euclidean distances and Ward’s clustering. (C) Hierarchical clustering heatmap analysis in the siRNA-transfected human subcutaneous adipocytes was performed using Ward’s method with the Euclidean distance. Heatmaps show the top 96 lipid species that differed in the siRNA-transfected human subcutaneous adipocytes. The number at the bottom indicates the replicate number. The color scale indicates the number of standard deviations from the overall average of the lipid species, with areas in dark red indicating a higher amount of the lipid species, whereas areas in dark blue indicate a lower amount of the lipid species. The red, green, and blue colors represent cells transfected with the siRNA-negative control, siRNA-<i>GNB1</i>, and siRNA-<i>SCARB2</i>, respectively. (D) Scatterplots of <i>P</i>-values were obtained using one-way analysis of variance (ANOVA) of the 366 lipid species in the siRNA-transfected human subcutaneous adipocytes. The intensity of the red dots corresponds to the –log<sub>10</sub> transformed <i>P</i>-values obtained using one-way ANOVA (–log<sub>10</sub> (raw <i>P</i>-value)). After FDR correction of the ANOVA results, Fisher’s LSD post hoc tests were performed on significant lipids to determine specific group differences. Both figures were generated using MetaboAnalyst 6.0. Abbreviations: siRNA-nc, siRNA-negative control; siRNA-G, siRNA-G protein subunit beta 1 (<i>GNB1</i>); siRNA-S, siRNA-scavenger receptor class B member 2 (<i>SCARB2</i>).</p> |
| eu_rights_str_mv | openAccess |
| id | Manara_4bc9f10e48586d26c901b6a10b66a50f |
| identifier_str_mv | 10.1371/journal.pone.0319163.g005 |
| network_acronym_str | Manara |
| network_name_str | ManaraRepo |
| oai_identifier_str | oai:figshare.com:article/28653622 |
| publishDate | 2025 |
| repository.mail.fl_str_mv | |
| repository.name.fl_str_mv | |
| repository_id_str | |
| rights_invalid_str_mv | CC BY 4.0 |
| spelling | Differential lipid species analysis in siRNA-transfected human subcutaneous adipocytes.Takuya Kitamoto (11721639)Aya Kitamoto (256033)BiochemistryCell BiologyGeneticsMolecular BiologyPhysiologyEnvironmental Sciences not elsewhere classifiedBiological Sciences not elsewhere classifiedChemical Sciences not elsewhere classifiedwide association studiesutilized rna interferencemolecular mechanisms linkinghuman subcutaneous adipocytesdiv >< pincluding genetic predispositionslipid droplet accumulationadipocyte lipid storageknockdown also ledalthough many obesityadipocyte fat accumulation>) — involvedfat accumulationlipid metabolismadipocyte functionproteins involvedvarious factorsupregulated followingsignificant alterationsscarb2 </providing insightslipidomic analysisknockdown resultedintegrative proteomicincluding plpp1gwas datagwas ),gnb1 </findings highlightcholesterol esters<p>(A) Partial Least Squares Discriminant Analysis (PLS-DA) of lipids in the siRNA-transfected human subcutaneous adipocytes. (B) Dendrogram showing the relationship between lipid species in the siRNA-transfected human subcutaneous adipocytes using Euclidean distances and Ward’s clustering. (C) Hierarchical clustering heatmap analysis in the siRNA-transfected human subcutaneous adipocytes was performed using Ward’s method with the Euclidean distance. Heatmaps show the top 96 lipid species that differed in the siRNA-transfected human subcutaneous adipocytes. The number at the bottom indicates the replicate number. The color scale indicates the number of standard deviations from the overall average of the lipid species, with areas in dark red indicating a higher amount of the lipid species, whereas areas in dark blue indicate a lower amount of the lipid species. The red, green, and blue colors represent cells transfected with the siRNA-negative control, siRNA-<i>GNB1</i>, and siRNA-<i>SCARB2</i>, respectively. (D) Scatterplots of <i>P</i>-values were obtained using one-way analysis of variance (ANOVA) of the 366 lipid species in the siRNA-transfected human subcutaneous adipocytes. The intensity of the red dots corresponds to the –log<sub>10</sub> transformed <i>P</i>-values obtained using one-way ANOVA (–log<sub>10</sub> (raw <i>P</i>-value)). After FDR correction of the ANOVA results, Fisher’s LSD post hoc tests were performed on significant lipids to determine specific group differences. Both figures were generated using MetaboAnalyst 6.0. Abbreviations: siRNA-nc, siRNA-negative control; siRNA-G, siRNA-G protein subunit beta 1 (<i>GNB1</i>); siRNA-S, siRNA-scavenger receptor class B member 2 (<i>SCARB2</i>).</p>2025-03-24T17:22:35ZImageFigureinfo:eu-repo/semantics/publishedVersionimage10.1371/journal.pone.0319163.g005https://figshare.com/articles/figure/Differential_lipid_species_analysis_in_siRNA-transfected_human_subcutaneous_adipocytes_/28653622CC BY 4.0info:eu-repo/semantics/openAccessoai:figshare.com:article/286536222025-03-24T17:22:35Z |
| spellingShingle | Differential lipid species analysis in siRNA-transfected human subcutaneous adipocytes. Takuya Kitamoto (11721639) Biochemistry Cell Biology Genetics Molecular Biology Physiology Environmental Sciences not elsewhere classified Biological Sciences not elsewhere classified Chemical Sciences not elsewhere classified wide association studies utilized rna interference molecular mechanisms linking human subcutaneous adipocytes div >< p including genetic predispositions lipid droplet accumulation adipocyte lipid storage knockdown also led although many obesity adipocyte fat accumulation >) — involved fat accumulation lipid metabolism adipocyte function proteins involved various factors upregulated following significant alterations scarb2 </ providing insights lipidomic analysis knockdown resulted integrative proteomic including plpp1 gwas data gwas ), gnb1 </ findings highlight cholesterol esters |
| status_str | publishedVersion |
| title | Differential lipid species analysis in siRNA-transfected human subcutaneous adipocytes. |
| title_full | Differential lipid species analysis in siRNA-transfected human subcutaneous adipocytes. |
| title_fullStr | Differential lipid species analysis in siRNA-transfected human subcutaneous adipocytes. |
| title_full_unstemmed | Differential lipid species analysis in siRNA-transfected human subcutaneous adipocytes. |
| title_short | Differential lipid species analysis in siRNA-transfected human subcutaneous adipocytes. |
| title_sort | Differential lipid species analysis in siRNA-transfected human subcutaneous adipocytes. |
| topic | Biochemistry Cell Biology Genetics Molecular Biology Physiology Environmental Sciences not elsewhere classified Biological Sciences not elsewhere classified Chemical Sciences not elsewhere classified wide association studies utilized rna interference molecular mechanisms linking human subcutaneous adipocytes div >< p including genetic predispositions lipid droplet accumulation adipocyte lipid storage knockdown also led although many obesity adipocyte fat accumulation >) — involved fat accumulation lipid metabolism adipocyte function proteins involved various factors upregulated following significant alterations scarb2 </ providing insights lipidomic analysis knockdown resulted integrative proteomic including plpp1 gwas data gwas ), gnb1 </ findings highlight cholesterol esters |