The result of enrich GO analysis.
<div><p>Cirrhosis is a common endpoint in various chronic liver diseases, and often causes hepatocellular carcinoma. Studies have revealed the significant role of disulfidptosis in the occurrence and development of hepatocellular carcinoma; however, our understanding of this role is limi...
محفوظ في:
| المؤلف الرئيسي: | |
|---|---|
| مؤلفون آخرون: | , , , |
| منشور في: |
2025
|
| الموضوعات: | |
| الوسوم: |
إضافة وسم
لا توجد وسوم, كن أول من يضع وسما على هذه التسجيلة!
|
| _version_ | 1852014928982966272 |
|---|---|
| author | Yiqian Liu (392940) |
| author2 | Ruixin Zhang (48813) Xiaojie Sun (583553) Wei Cui (92129) Lixin Liu (676169) |
| author2_role | author author author author |
| author_facet | Yiqian Liu (392940) Ruixin Zhang (48813) Xiaojie Sun (583553) Wei Cui (92129) Lixin Liu (676169) |
| author_role | author |
| dc.creator.none.fl_str_mv | Yiqian Liu (392940) Ruixin Zhang (48813) Xiaojie Sun (583553) Wei Cui (92129) Lixin Liu (676169) |
| dc.date.none.fl_str_mv | 2025-11-12T18:26:54Z |
| dc.identifier.none.fl_str_mv | 10.1371/journal.pone.0334459.s007 |
| dc.relation.none.fl_str_mv | https://figshare.com/articles/dataset/The_result_of_enrich_GO_analysis_/30602401 |
| dc.rights.none.fl_str_mv | CC BY 4.0 info:eu-repo/semantics/openAccess |
| dc.subject.none.fl_str_mv | Biophysics Biochemistry Microbiology Cell Biology Genetics Molecular Biology Immunology Biological Sciences not elsewhere classified weighted gene co networks using genemania immunohistochemical experiments confirmed gene expression omnibus gene expression data expression network analysis experimental evidence confirm immune cell populations immune cell infiltration novel diagnostic biomarkers new therapeutic targets genes mainly promote differentially expressed genes div >< p identify potential disulfidptosis immune cells therapeutic target related biomarkers identify hub shared genes hub genes strong useful sample gsea related signature potential utility oxidative metabolism hepatocellular carcinoma gsva ). gsea ), efficient disulfidptosis early diagnosis cytokine pathways cxcl8 </ common endpoint 28 types |
| dc.title.none.fl_str_mv | The result of enrich GO analysis. |
| dc.type.none.fl_str_mv | Dataset info:eu-repo/semantics/publishedVersion dataset |
| description | <div><p>Cirrhosis is a common endpoint in various chronic liver diseases, and often causes hepatocellular carcinoma. Studies have revealed the significant role of disulfidptosis in the occurrence and development of hepatocellular carcinoma; however, our understanding of this role is limited. Therefore, we aimed to identify potential disulfidptosis-related biomarkers for cirrhosis. We obtained the gene expression data of patients with cirrhosis from the Gene Expression Omnibus (GEO) database. Subsequently, weighted gene co-expression network analysis was performed, and the “limma” package was used to screen for differentially expressed genes (DEGs) associated with disulfidptosis. Significantly altered biological pathways were identified using Gene Ontology (GO), Kyoto Encyclopedia of Genes and Genomes (KEGG), Gene Set Enrichment Analysis (GSEA), and Gene Set Variation Analysis (GSVA). We constructed protein–protein interaction (PPI) networks using GeneMANIA and generated receiver operating characteristic (ROC) curves to identify hub-shared genes. Additionally, we assessed the distribution of immune cell populations in cirrhotic and control specimens using single-sample GSEA (ssGSEA) and explored their relationship with hub genes. Six hub genes (<i>CXCL12, COL1A1, CXCR4, COL1A2, CCR7,</i> and <i>CXCL8</i>) were closely associated with disulfidptosis-related DEGs. Further immunohistochemical experiments confirmed the potential of <i>CCR7, CXCL12, CXCR4,</i> and <i>CXCL8</i> as novel diagnostic biomarkers and suggested their potential as new therapeutic targets. These genes mainly promote the development of liver cirrhosis through the oxidative metabolism and cytokine pathways. Furthermore, we observed positive correlations among 23 of the 28 types of immune cells. This study highlights the potential utility of immune cell infiltration and efficient disulfidptosis-related early diagnostic biomarkers in cirrhosis, and highlights its strong useful as a therapeutic target, offering potential clinical application value.</p></div> |
| eu_rights_str_mv | openAccess |
| id | Manara_4d95dc80a0e0dcc2f34ca60e707aaa66 |
| identifier_str_mv | 10.1371/journal.pone.0334459.s007 |
| network_acronym_str | Manara |
| network_name_str | ManaraRepo |
| oai_identifier_str | oai:figshare.com:article/30602401 |
| publishDate | 2025 |
| repository.mail.fl_str_mv | |
| repository.name.fl_str_mv | |
| repository_id_str | |
| rights_invalid_str_mv | CC BY 4.0 |
| spelling | The result of enrich GO analysis.Yiqian Liu (392940)Ruixin Zhang (48813)Xiaojie Sun (583553)Wei Cui (92129)Lixin Liu (676169)BiophysicsBiochemistryMicrobiologyCell BiologyGeneticsMolecular BiologyImmunologyBiological Sciences not elsewhere classifiedweighted gene conetworks using genemaniaimmunohistochemical experiments confirmedgene expression omnibusgene expression dataexpression network analysisexperimental evidence confirmimmune cell populationsimmune cell infiltrationnovel diagnostic biomarkersnew therapeutic targetsgenes mainly promotedifferentially expressed genesdiv >< pidentify potential disulfidptosisimmune cellstherapeutic targetrelated biomarkersidentify hubshared geneshub genesstrong usefulsample gsearelated signaturepotential utilityoxidative metabolismhepatocellular carcinomagsva ).gsea ),efficient disulfidptosisearly diagnosiscytokine pathwayscxcl8 </common endpoint28 types<div><p>Cirrhosis is a common endpoint in various chronic liver diseases, and often causes hepatocellular carcinoma. Studies have revealed the significant role of disulfidptosis in the occurrence and development of hepatocellular carcinoma; however, our understanding of this role is limited. Therefore, we aimed to identify potential disulfidptosis-related biomarkers for cirrhosis. We obtained the gene expression data of patients with cirrhosis from the Gene Expression Omnibus (GEO) database. Subsequently, weighted gene co-expression network analysis was performed, and the “limma” package was used to screen for differentially expressed genes (DEGs) associated with disulfidptosis. Significantly altered biological pathways were identified using Gene Ontology (GO), Kyoto Encyclopedia of Genes and Genomes (KEGG), Gene Set Enrichment Analysis (GSEA), and Gene Set Variation Analysis (GSVA). We constructed protein–protein interaction (PPI) networks using GeneMANIA and generated receiver operating characteristic (ROC) curves to identify hub-shared genes. Additionally, we assessed the distribution of immune cell populations in cirrhotic and control specimens using single-sample GSEA (ssGSEA) and explored their relationship with hub genes. Six hub genes (<i>CXCL12, COL1A1, CXCR4, COL1A2, CCR7,</i> and <i>CXCL8</i>) were closely associated with disulfidptosis-related DEGs. Further immunohistochemical experiments confirmed the potential of <i>CCR7, CXCL12, CXCR4,</i> and <i>CXCL8</i> as novel diagnostic biomarkers and suggested their potential as new therapeutic targets. These genes mainly promote the development of liver cirrhosis through the oxidative metabolism and cytokine pathways. Furthermore, we observed positive correlations among 23 of the 28 types of immune cells. This study highlights the potential utility of immune cell infiltration and efficient disulfidptosis-related early diagnostic biomarkers in cirrhosis, and highlights its strong useful as a therapeutic target, offering potential clinical application value.</p></div>2025-11-12T18:26:54ZDatasetinfo:eu-repo/semantics/publishedVersiondataset10.1371/journal.pone.0334459.s007https://figshare.com/articles/dataset/The_result_of_enrich_GO_analysis_/30602401CC BY 4.0info:eu-repo/semantics/openAccessoai:figshare.com:article/306024012025-11-12T18:26:54Z |
| spellingShingle | The result of enrich GO analysis. Yiqian Liu (392940) Biophysics Biochemistry Microbiology Cell Biology Genetics Molecular Biology Immunology Biological Sciences not elsewhere classified weighted gene co networks using genemania immunohistochemical experiments confirmed gene expression omnibus gene expression data expression network analysis experimental evidence confirm immune cell populations immune cell infiltration novel diagnostic biomarkers new therapeutic targets genes mainly promote differentially expressed genes div >< p identify potential disulfidptosis immune cells therapeutic target related biomarkers identify hub shared genes hub genes strong useful sample gsea related signature potential utility oxidative metabolism hepatocellular carcinoma gsva ). gsea ), efficient disulfidptosis early diagnosis cytokine pathways cxcl8 </ common endpoint 28 types |
| status_str | publishedVersion |
| title | The result of enrich GO analysis. |
| title_full | The result of enrich GO analysis. |
| title_fullStr | The result of enrich GO analysis. |
| title_full_unstemmed | The result of enrich GO analysis. |
| title_short | The result of enrich GO analysis. |
| title_sort | The result of enrich GO analysis. |
| topic | Biophysics Biochemistry Microbiology Cell Biology Genetics Molecular Biology Immunology Biological Sciences not elsewhere classified weighted gene co networks using genemania immunohistochemical experiments confirmed gene expression omnibus gene expression data expression network analysis experimental evidence confirm immune cell populations immune cell infiltration novel diagnostic biomarkers new therapeutic targets genes mainly promote differentially expressed genes div >< p identify potential disulfidptosis immune cells therapeutic target related biomarkers identify hub shared genes hub genes strong useful sample gsea related signature potential utility oxidative metabolism hepatocellular carcinoma gsva ). gsea ), efficient disulfidptosis early diagnosis cytokine pathways cxcl8 </ common endpoint 28 types |