DBT9-<i>Hs</i>ACE2 cells were pretreated with 20 mM NH<sub>4</sub>Cl or DMEM for 20 min prior to addition of genome-normalized scVSV particles bearing Rs3367-CoV(WT) or Rs3367-CoV(Y623H) spikes in media with or without 20mM NH<sub>4</sub>Cl.

DBT9-<i>Hs</i>ACE2 cells were pretreated with 20 mM NH<sub>4</sub>Cl or DMEM for 20 min prior to addition of genome-normalized scVSV particles bearing Rs3367-CoV(WT) or Rs3367-CoV(Y623H) spikes in media with or without 20mM NH<sub>4</sub>Cl.

<p>Infection was scored by eGFP expression at 16–18 hours post-infection (average±SD. n = 4 from 2 independent experiments).</p> <p>(TIF)</p>

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Bibliographic Details
Main Author: Alexandra L. Tse (20235212) (author)
Other Authors: Gorka Lasso (215085) (author), Jacob Berrigan (11052951) (author), Jason S. McLellan (8816639) (author), Kartik Chandran (134142) (author), Emily Happy Miller (10652108) (author)
Published: 2025
Subjects:
Neuroscience
Evolutionary Biology
Cancer
Infectious Diseases
Virology
significant zoonotic threat
mammalian respiratory tract
host protease barrier
genetic pathways associated
even initially replace
poor entry activity
key viral adaptation
entry could precede
alters spike dynamics
increased spike opening
enhances spike opening
xlink "> sars
s1 &# 8211
loop &# 8217
enhance spike cleavage
opening substitutions arose
bat sarbecovirus wiv1
cov spikes could
viral entry
&# 8217
opening substitutions
s2 cleavage
s1 subunit
cell entry
&# 8216
type spike
spike resembling
successful isolation
single amino
s2 boundary
rhinolophid bats
pseudotype system
previously showed
previously identified
oral lifestyle
natural hosts
like betacoronaviruses
incompletely understood
immune evasion
findings suggest
currently recognized
cov using
cov bears
conformational constraints
cell culture
broader property
binding domain
bats pose
bat sarbecoviruses
adaptive mutation
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