FIS1 and MiD49 as the adaptors of DRP1 mediates PRRSV-induced mitochondrial fission.
<p>(A-B) Marc-145 cells were transfected with control siRNA or siRNA targeting FIS1 (A) or MiD49 (B) for 24 h, and then were either mock-infected or infected with PRRSV (0.1 MOI) for an additional 24 h. The cells were stained with MitoTracker Red, PRRSV-N antibody, and DAPI, followed by confoc...
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2025
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| _version_ | 1852023638744629248 |
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| author | Zhe Sun (328682) |
| author2 | Zicheng Ma (14071216) Wandi Cao (20553288) Chenlong Jiang (14071219) Lei Guo (36221) Kesen Liu (9086343) Yanni Gao (6250973) Juan Bai (798838) Jiang Pi (817619) Ping Jiang (243020) Xing Liu (221084) |
| author2_role | author author author author author author author author author author |
| author_facet | Zhe Sun (328682) Zicheng Ma (14071216) Wandi Cao (20553288) Chenlong Jiang (14071219) Lei Guo (36221) Kesen Liu (9086343) Yanni Gao (6250973) Juan Bai (798838) Jiang Pi (817619) Ping Jiang (243020) Xing Liu (221084) |
| author_role | author |
| dc.creator.none.fl_str_mv | Zhe Sun (328682) Zicheng Ma (14071216) Wandi Cao (20553288) Chenlong Jiang (14071219) Lei Guo (36221) Kesen Liu (9086343) Yanni Gao (6250973) Juan Bai (798838) Jiang Pi (817619) Ping Jiang (243020) Xing Liu (221084) |
| dc.date.none.fl_str_mv | 2025-01-13T19:09:11Z |
| dc.identifier.none.fl_str_mv | 10.1371/journal.ppat.1012872.g007 |
| dc.relation.none.fl_str_mv | https://figshare.com/articles/figure/FIS1_and_MiD49_as_the_adaptors_of_DRP1_mediates_PRRSV-induced_mitochondrial_fission_/28199208 |
| dc.rights.none.fl_str_mv | CC BY 4.0 info:eu-repo/semantics/openAccess |
| dc.subject.none.fl_str_mv | Biophysics Biochemistry Cell Biology Genetics Molecular Biology Neuroscience Physiology Cancer Science Policy Infectious Diseases Plant Biology Virology Environmental Sciences not elsewhere classified “ powerhouse ” providing new insights promoting cellular glycolysis mams ), mediated ip3r )— voltage generating cellular energy excessive lactate production camkkβ )— amp ampk )— dynamin activated protein kinase regulates mitochondrial function mitochondrial dynamics proteins hijack mitochondrial function promote mitochondrial fission mediated mitochondrial fission induced mitochondrial fission induce mitochondrial fission alongside mitochondrial fission related protein 1 triggers mitophagy via mitophagy drive glycolysis respiratory syndrome virus div >< p 2 +</ sup elevated mitochondria ca mitochondrial ca inositol 1 elevated levels vital role upregulated expression triphosphate receptor study reveals sigmar1 ). sigma non mitochondria ca increased mitochondria evolved mechanisms endoplasmic reticulum dynamic processes 49 kda |
| dc.title.none.fl_str_mv | FIS1 and MiD49 as the adaptors of DRP1 mediates PRRSV-induced mitochondrial fission. |
| dc.type.none.fl_str_mv | Image Figure info:eu-repo/semantics/publishedVersion image |
| description | <p>(A-B) Marc-145 cells were transfected with control siRNA or siRNA targeting FIS1 (A) or MiD49 (B) for 24 h, and then were either mock-infected or infected with PRRSV (0.1 MOI) for an additional 24 h. The cells were stained with MitoTracker Red, PRRSV-N antibody, and DAPI, followed by confocal microscopy. Quantitative analysis of mitochondrial length is presented for 25 cells per group (right panel). (C-D) Marc-145 cells were mock-infected or infected with PRRSV (MOI = 0.1) for 24 h. The cells were lysed and precipitated using anti-FIS1 or anti-MiD49 antibodies. The whole-cell lysates (WCL) and immunoprecipitated proteins were analyzed using specific antibodies. Anti-Rabbit immunoglobulin G (IgG) antibody was used as a negative control. (E and F) After 24 hours of siRNA FIS1 (E) and MiD49 (F) transfection, the cells were either mock-infected or infected with PRRSV (0.1 MOI) for an additional 24 hours, and then harvested for cytoplasmic and mitochondrial fractionation. Then samples were subjected to western blot analysis. The levels of proteins were normalized to Tom20 or α-tubulin. Data are expressed as means ± SD, n = 25 in A and B. *p<0.05; **p < 0.01; ***p < 0.001. The data are representative of results from three independent experiments.</p> |
| eu_rights_str_mv | openAccess |
| id | Manara_4e83da3e4deb15e8980c0c8a9c8075bb |
| identifier_str_mv | 10.1371/journal.ppat.1012872.g007 |
| network_acronym_str | Manara |
| network_name_str | ManaraRepo |
| oai_identifier_str | oai:figshare.com:article/28199208 |
| publishDate | 2025 |
| repository.mail.fl_str_mv | |
| repository.name.fl_str_mv | |
| repository_id_str | |
| rights_invalid_str_mv | CC BY 4.0 |
| spelling | FIS1 and MiD49 as the adaptors of DRP1 mediates PRRSV-induced mitochondrial fission.Zhe Sun (328682)Zicheng Ma (14071216)Wandi Cao (20553288)Chenlong Jiang (14071219)Lei Guo (36221)Kesen Liu (9086343)Yanni Gao (6250973)Juan Bai (798838)Jiang Pi (817619)Ping Jiang (243020)Xing Liu (221084)BiophysicsBiochemistryCell BiologyGeneticsMolecular BiologyNeurosciencePhysiologyCancerScience PolicyInfectious DiseasesPlant BiologyVirologyEnvironmental Sciences not elsewhere classified“ powerhouse ”providing new insightspromoting cellular glycolysismams ), mediatedip3r )— voltagegenerating cellular energyexcessive lactate productioncamkkβ )— ampampk )— dynaminactivated protein kinaseregulates mitochondrial functionmitochondrial dynamics proteinshijack mitochondrial functionpromote mitochondrial fissionmediated mitochondrial fissioninduced mitochondrial fissioninduce mitochondrial fissionalongside mitochondrial fissionrelated protein 1triggers mitophagy viamitophagy drive glycolysisrespiratory syndrome virusdiv >< p2 +</ supelevated mitochondria camitochondrial cainositol 1elevated levelsvital roleupregulated expressiontriphosphate receptorstudy revealssigmar1 ).sigma nonmitochondria caincreased mitochondriaevolved mechanismsendoplasmic reticulumdynamic processes49 kda<p>(A-B) Marc-145 cells were transfected with control siRNA or siRNA targeting FIS1 (A) or MiD49 (B) for 24 h, and then were either mock-infected or infected with PRRSV (0.1 MOI) for an additional 24 h. The cells were stained with MitoTracker Red, PRRSV-N antibody, and DAPI, followed by confocal microscopy. Quantitative analysis of mitochondrial length is presented for 25 cells per group (right panel). (C-D) Marc-145 cells were mock-infected or infected with PRRSV (MOI = 0.1) for 24 h. The cells were lysed and precipitated using anti-FIS1 or anti-MiD49 antibodies. The whole-cell lysates (WCL) and immunoprecipitated proteins were analyzed using specific antibodies. Anti-Rabbit immunoglobulin G (IgG) antibody was used as a negative control. (E and F) After 24 hours of siRNA FIS1 (E) and MiD49 (F) transfection, the cells were either mock-infected or infected with PRRSV (0.1 MOI) for an additional 24 hours, and then harvested for cytoplasmic and mitochondrial fractionation. Then samples were subjected to western blot analysis. The levels of proteins were normalized to Tom20 or α-tubulin. Data are expressed as means ± SD, n = 25 in A and B. *p<0.05; **p < 0.01; ***p < 0.001. The data are representative of results from three independent experiments.</p>2025-01-13T19:09:11ZImageFigureinfo:eu-repo/semantics/publishedVersionimage10.1371/journal.ppat.1012872.g007https://figshare.com/articles/figure/FIS1_and_MiD49_as_the_adaptors_of_DRP1_mediates_PRRSV-induced_mitochondrial_fission_/28199208CC BY 4.0info:eu-repo/semantics/openAccessoai:figshare.com:article/281992082025-01-13T19:09:11Z |
| spellingShingle | FIS1 and MiD49 as the adaptors of DRP1 mediates PRRSV-induced mitochondrial fission. Zhe Sun (328682) Biophysics Biochemistry Cell Biology Genetics Molecular Biology Neuroscience Physiology Cancer Science Policy Infectious Diseases Plant Biology Virology Environmental Sciences not elsewhere classified “ powerhouse ” providing new insights promoting cellular glycolysis mams ), mediated ip3r )— voltage generating cellular energy excessive lactate production camkkβ )— amp ampk )— dynamin activated protein kinase regulates mitochondrial function mitochondrial dynamics proteins hijack mitochondrial function promote mitochondrial fission mediated mitochondrial fission induced mitochondrial fission induce mitochondrial fission alongside mitochondrial fission related protein 1 triggers mitophagy via mitophagy drive glycolysis respiratory syndrome virus div >< p 2 +</ sup elevated mitochondria ca mitochondrial ca inositol 1 elevated levels vital role upregulated expression triphosphate receptor study reveals sigmar1 ). sigma non mitochondria ca increased mitochondria evolved mechanisms endoplasmic reticulum dynamic processes 49 kda |
| status_str | publishedVersion |
| title | FIS1 and MiD49 as the adaptors of DRP1 mediates PRRSV-induced mitochondrial fission. |
| title_full | FIS1 and MiD49 as the adaptors of DRP1 mediates PRRSV-induced mitochondrial fission. |
| title_fullStr | FIS1 and MiD49 as the adaptors of DRP1 mediates PRRSV-induced mitochondrial fission. |
| title_full_unstemmed | FIS1 and MiD49 as the adaptors of DRP1 mediates PRRSV-induced mitochondrial fission. |
| title_short | FIS1 and MiD49 as the adaptors of DRP1 mediates PRRSV-induced mitochondrial fission. |
| title_sort | FIS1 and MiD49 as the adaptors of DRP1 mediates PRRSV-induced mitochondrial fission. |
| topic | Biophysics Biochemistry Cell Biology Genetics Molecular Biology Neuroscience Physiology Cancer Science Policy Infectious Diseases Plant Biology Virology Environmental Sciences not elsewhere classified “ powerhouse ” providing new insights promoting cellular glycolysis mams ), mediated ip3r )— voltage generating cellular energy excessive lactate production camkkβ )— amp ampk )— dynamin activated protein kinase regulates mitochondrial function mitochondrial dynamics proteins hijack mitochondrial function promote mitochondrial fission mediated mitochondrial fission induced mitochondrial fission induce mitochondrial fission alongside mitochondrial fission related protein 1 triggers mitophagy via mitophagy drive glycolysis respiratory syndrome virus div >< p 2 +</ sup elevated mitochondria ca mitochondrial ca inositol 1 elevated levels vital role upregulated expression triphosphate receptor study reveals sigmar1 ). sigma non mitochondria ca increased mitochondria evolved mechanisms endoplasmic reticulum dynamic processes 49 kda |