Local Means Thresholding Algorithm shows high robustness.
<p>(A) Example straightened crops of 63X confocal images from the Overexpressed Synaptic Fluorophore Dataset showing CLA-1::GFP-labeled presynapses in the DA9 neurons of control worms and worms with of two different mutant alleles that show severe [syd-1(-)] or mild [nrx-1(-)] loss of puncta....
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2025
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| Summary: | <p>(A) Example straightened crops of 63X confocal images from the Overexpressed Synaptic Fluorophore Dataset showing CLA-1::GFP-labeled presynapses in the DA9 neurons of control worms and worms with of two different mutant alleles that show severe [syd-1(-)] or mild [nrx-1(-)] loss of puncta. Scale Bars, 5µm. (B) Examples of ROIs detected in the boxed region of each crop in A based on minimum (0.05, red), ideal (0.2, green), and maximum (0.35, blue) sensitivity settings for ROI thresholding. Red arrows indicate ROIs that were detected at the ideal sensitivity but not at the minimum sensitivity. Green arrows indicate ROIs that were detected at the maximum sensitivity but not at the ideal sensitivity. Scale Bars, 5µm. (C) Graphs of ROI counts for the Overexpressed Synaptic Fluorophore control and mutants with loss of puncta phenotypes at the three selected sensitivities. Number of worms analyzed in each genotype shown at the bottom of each bar graph. (* = p < 0.05, ** = p < 0.01, *** = p < 0.001, **** = p < 0.0001). (D) Example sections of straightened crops of 100X confocal images from the Endogenous Synaptic Fluorophore Datasets showing NRX-1::Skylan-S-labeled presynapses in the dorsal nerve cords (DNC) of control worms and worms with of two different mutant alleles that show severe [syd-1(∆PDZ)] or mild [syd-1(∆C2)] disruption in NRX-1clustering. Scale Bar 5µm. (E) Examples of ROIs detected in the boxed region of the of each crop in D based on minimum (0.2, red), ideal (0.35, green), and maximum (0.5, blue) sensitivity settings for ROI thresholding. Red arrows indicate ROIs that were detected at the ideal sensitivity but not at the minimum sensitivity. Green arrows indicate ROIs that were detected at the maximum sensitivity but not at the ideal sensitivity. Scale Bars, 5µm. (F) Graphs of Normalized ROI count per 100µm (along entire crop) for Endogenous Synaptic Fluorophore control and mutants with clustering phenotypes at the three selected sensitivities. Number of worms analyzed in each genotype shown at the bottom of each bar graph. (* = p < 0.05, ** = p < 0.01, *** = p < 0.001, **** = p < 0.0001).</p> |
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