Structural organization of distal ring and hook elements in mouse tracheal epithelial cells.
<p><b>(A, B)</b> Nine-fold symmetrized cryo-tomogram slices of a centriole from mouse tracheal epithelial cells (MTECs). (A) The luminal ring structure is not visible, while the microtubule-associated “hook” structures are observed (pink arrowheads). (B) Both the luminal ring (oran...
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2025
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| Summary: | <p><b>(A, B)</b> Nine-fold symmetrized cryo-tomogram slices of a centriole from mouse tracheal epithelial cells (MTECs). (A) The luminal ring structure is not visible, while the microtubule-associated “hook” structures are observed (pink arrowheads). (B) Both the luminal ring (orange arrowhead) and the hook structures (pink arrowheads) are visible and positioned within the same z-plane, indicating their coplanarity. White arrowheads mark microtubule triplets or doublets [<a href="http://www.plosbiology.org/article/info:doi/10.1371/journal.pbio.3003519#pbio.3003519.ref067" target="_blank">67</a>]. <b>(C)</b> Relative position of the hook structures with respect to the luminal ring. The graph shows the measured distances along the microtubule from the ring structure to the distal end of the hook structures, with an average distance of 28.96 nm ± 7.58 nm. <b>(D)</b> Fourier Shell Correlation (FSC) resolution estimation of the subtomograms average centered on the hook density between microtubule doublets/triplets. Resolution: 36 Å at an FSC cutoff of 0.143. The 3D reconstruction (right) shows continuous hook density along the A-microtubule wall (pink arrowheads). <b>(E)</b> FSC resolution estimation of the subtomograms average centered on the ring structure, containing three node densities. Resolution: 38 Å at an FSC cutoff of 0.143. The corresponding 3D map (right) highlights the amorphous density (blue arrowheads) and the structured ring elements (orange arrowheads). The detailed statistics of all the graphs shown in the figure are included in the S1 Data file.</p> <p>(TIFF)</p> |
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