Structural organization of distal ring and hook elements in mouse tracheal epithelial cells.
<p><b>(A, B)</b> Nine-fold symmetrized cryo-tomogram slices of a centriole from mouse tracheal epithelial cells (MTECs). (A) The luminal ring structure is not visible, while the microtubule-associated “hook” structures are observed (pink arrowheads). (B) Both the luminal ring (oran...
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2025
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| _version_ | 1852014066273353728 |
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| author | Eloïse Bertiaux (5309927) |
| author2 | Vincent Louvel (10291256) Caitlyn L. McCafferty (4669267) Hugo van den Hoek (22813456) Umut Batman (13113614) Souradip Mukherjee (22813459) Lorène Bournonville (10291253) Olivier Mercey (713210) Isabelle Méan (11411492) Ricardo D. Righetto (22813462) Adrian Müller (22813465) Philippe Van der Stappen (22813468) Garrison Buss (22813471) Jean Daraspe (5574758) Christel Genoud (66232) Tim Stearns (106556) Benjamin D. Engel (22813474) Virginie Hamel (10291262) Paul Guichard (10291259) |
| author2_role | author author author author author author author author author author author author author author author author author author |
| author_facet | Eloïse Bertiaux (5309927) Vincent Louvel (10291256) Caitlyn L. McCafferty (4669267) Hugo van den Hoek (22813456) Umut Batman (13113614) Souradip Mukherjee (22813459) Lorène Bournonville (10291253) Olivier Mercey (713210) Isabelle Méan (11411492) Ricardo D. Righetto (22813462) Adrian Müller (22813465) Philippe Van der Stappen (22813468) Garrison Buss (22813471) Jean Daraspe (5574758) Christel Genoud (66232) Tim Stearns (106556) Benjamin D. Engel (22813474) Virginie Hamel (10291262) Paul Guichard (10291259) |
| author_role | author |
| dc.creator.none.fl_str_mv | Eloïse Bertiaux (5309927) Vincent Louvel (10291256) Caitlyn L. McCafferty (4669267) Hugo van den Hoek (22813456) Umut Batman (13113614) Souradip Mukherjee (22813459) Lorène Bournonville (10291253) Olivier Mercey (713210) Isabelle Méan (11411492) Ricardo D. Righetto (22813462) Adrian Müller (22813465) Philippe Van der Stappen (22813468) Garrison Buss (22813471) Jean Daraspe (5574758) Christel Genoud (66232) Tim Stearns (106556) Benjamin D. Engel (22813474) Virginie Hamel (10291262) Paul Guichard (10291259) |
| dc.date.none.fl_str_mv | 2025-12-09T19:00:06Z |
| dc.identifier.none.fl_str_mv | 10.1371/journal.pbio.3003519.s003 |
| dc.relation.none.fl_str_mv | https://figshare.com/articles/figure/Structural_organization_of_distal_ring_and_hook_elements_in_mouse_tracheal_epithelial_cells_/30840496 |
| dc.rights.none.fl_str_mv | CC BY 4.0 info:eu-repo/semantics/openAccess |
| dc.subject.none.fl_str_mv | Biophysics Biochemistry Microbiology Cell Biology Molecular Biology Biotechnology Evolutionary Biology Infectious Diseases precise molecular role fold organization within intricate &# 8220 xlink "> centrioles radially symmetric 9 molecular hub connecting luminal ring structure c2cd3 localizes close c2cd3 depletion disrupts centriole &# 8217 distal microtubule cap appendages </ p distal appendage assembly &# 8221 shorter centrioles architectural hub appendage defects distal lumen distal end distal centriole like structure protein c2cd3 c2cd3 forms c2cd3 adopts triplet microtubules terminal region polarized microtubule orofaciodigital syndrome ofd1 ), mutations linked mouse tissues human cells extends radially electron tomography disco complex cilia formation centriole interior centriolar lumen based structures also destabilizes 27 nodes |
| dc.title.none.fl_str_mv | Structural organization of distal ring and hook elements in mouse tracheal epithelial cells. |
| dc.type.none.fl_str_mv | Image Figure info:eu-repo/semantics/publishedVersion image |
| description | <p><b>(A, B)</b> Nine-fold symmetrized cryo-tomogram slices of a centriole from mouse tracheal epithelial cells (MTECs). (A) The luminal ring structure is not visible, while the microtubule-associated “hook” structures are observed (pink arrowheads). (B) Both the luminal ring (orange arrowhead) and the hook structures (pink arrowheads) are visible and positioned within the same z-plane, indicating their coplanarity. White arrowheads mark microtubule triplets or doublets [<a href="http://www.plosbiology.org/article/info:doi/10.1371/journal.pbio.3003519#pbio.3003519.ref067" target="_blank">67</a>]. <b>(C)</b> Relative position of the hook structures with respect to the luminal ring. The graph shows the measured distances along the microtubule from the ring structure to the distal end of the hook structures, with an average distance of 28.96 nm ± 7.58 nm. <b>(D)</b> Fourier Shell Correlation (FSC) resolution estimation of the subtomograms average centered on the hook density between microtubule doublets/triplets. Resolution: 36 Å at an FSC cutoff of 0.143. The 3D reconstruction (right) shows continuous hook density along the A-microtubule wall (pink arrowheads). <b>(E)</b> FSC resolution estimation of the subtomograms average centered on the ring structure, containing three node densities. Resolution: 38 Å at an FSC cutoff of 0.143. The corresponding 3D map (right) highlights the amorphous density (blue arrowheads) and the structured ring elements (orange arrowheads). The detailed statistics of all the graphs shown in the figure are included in the S1 Data file.</p> <p>(TIFF)</p> |
| eu_rights_str_mv | openAccess |
| id | Manara_516269b3faab0ba4f2fd02aa4e73dd1f |
| identifier_str_mv | 10.1371/journal.pbio.3003519.s003 |
| network_acronym_str | Manara |
| network_name_str | ManaraRepo |
| oai_identifier_str | oai:figshare.com:article/30840496 |
| publishDate | 2025 |
| repository.mail.fl_str_mv | |
| repository.name.fl_str_mv | |
| repository_id_str | |
| rights_invalid_str_mv | CC BY 4.0 |
| spelling | Structural organization of distal ring and hook elements in mouse tracheal epithelial cells.Eloïse Bertiaux (5309927)Vincent Louvel (10291256)Caitlyn L. McCafferty (4669267)Hugo van den Hoek (22813456)Umut Batman (13113614)Souradip Mukherjee (22813459)Lorène Bournonville (10291253)Olivier Mercey (713210)Isabelle Méan (11411492)Ricardo D. Righetto (22813462)Adrian Müller (22813465)Philippe Van der Stappen (22813468)Garrison Buss (22813471)Jean Daraspe (5574758)Christel Genoud (66232)Tim Stearns (106556)Benjamin D. Engel (22813474)Virginie Hamel (10291262)Paul Guichard (10291259)BiophysicsBiochemistryMicrobiologyCell BiologyMolecular BiologyBiotechnologyEvolutionary BiologyInfectious Diseasesprecise molecular rolefold organization withinintricate &# 8220xlink "> centriolesradially symmetric 9molecular hub connectingluminal ring structurec2cd3 localizes closec2cd3 depletion disruptscentriole &# 8217distal microtubule capappendages </ pdistal appendage assembly&# 8221shorter centriolesarchitectural hubappendage defectsdistal lumendistal enddistal centriolelike structureprotein c2cd3c2cd3 formsc2cd3 adoptstriplet microtubulesterminal regionpolarized microtubuleorofaciodigital syndromeofd1 ),mutations linkedmouse tissueshuman cellsextends radiallyelectron tomographydisco complexcilia formationcentriole interiorcentriolar lumenbased structuresalso destabilizes27 nodes<p><b>(A, B)</b> Nine-fold symmetrized cryo-tomogram slices of a centriole from mouse tracheal epithelial cells (MTECs). (A) The luminal ring structure is not visible, while the microtubule-associated “hook” structures are observed (pink arrowheads). (B) Both the luminal ring (orange arrowhead) and the hook structures (pink arrowheads) are visible and positioned within the same z-plane, indicating their coplanarity. White arrowheads mark microtubule triplets or doublets [<a href="http://www.plosbiology.org/article/info:doi/10.1371/journal.pbio.3003519#pbio.3003519.ref067" target="_blank">67</a>]. <b>(C)</b> Relative position of the hook structures with respect to the luminal ring. The graph shows the measured distances along the microtubule from the ring structure to the distal end of the hook structures, with an average distance of 28.96 nm ± 7.58 nm. <b>(D)</b> Fourier Shell Correlation (FSC) resolution estimation of the subtomograms average centered on the hook density between microtubule doublets/triplets. Resolution: 36 Å at an FSC cutoff of 0.143. The 3D reconstruction (right) shows continuous hook density along the A-microtubule wall (pink arrowheads). <b>(E)</b> FSC resolution estimation of the subtomograms average centered on the ring structure, containing three node densities. Resolution: 38 Å at an FSC cutoff of 0.143. The corresponding 3D map (right) highlights the amorphous density (blue arrowheads) and the structured ring elements (orange arrowheads). The detailed statistics of all the graphs shown in the figure are included in the S1 Data file.</p> <p>(TIFF)</p>2025-12-09T19:00:06ZImageFigureinfo:eu-repo/semantics/publishedVersionimage10.1371/journal.pbio.3003519.s003https://figshare.com/articles/figure/Structural_organization_of_distal_ring_and_hook_elements_in_mouse_tracheal_epithelial_cells_/30840496CC BY 4.0info:eu-repo/semantics/openAccessoai:figshare.com:article/308404962025-12-09T19:00:06Z |
| spellingShingle | Structural organization of distal ring and hook elements in mouse tracheal epithelial cells. Eloïse Bertiaux (5309927) Biophysics Biochemistry Microbiology Cell Biology Molecular Biology Biotechnology Evolutionary Biology Infectious Diseases precise molecular role fold organization within intricate &# 8220 xlink "> centrioles radially symmetric 9 molecular hub connecting luminal ring structure c2cd3 localizes close c2cd3 depletion disrupts centriole &# 8217 distal microtubule cap appendages </ p distal appendage assembly &# 8221 shorter centrioles architectural hub appendage defects distal lumen distal end distal centriole like structure protein c2cd3 c2cd3 forms c2cd3 adopts triplet microtubules terminal region polarized microtubule orofaciodigital syndrome ofd1 ), mutations linked mouse tissues human cells extends radially electron tomography disco complex cilia formation centriole interior centriolar lumen based structures also destabilizes 27 nodes |
| status_str | publishedVersion |
| title | Structural organization of distal ring and hook elements in mouse tracheal epithelial cells. |
| title_full | Structural organization of distal ring and hook elements in mouse tracheal epithelial cells. |
| title_fullStr | Structural organization of distal ring and hook elements in mouse tracheal epithelial cells. |
| title_full_unstemmed | Structural organization of distal ring and hook elements in mouse tracheal epithelial cells. |
| title_short | Structural organization of distal ring and hook elements in mouse tracheal epithelial cells. |
| title_sort | Structural organization of distal ring and hook elements in mouse tracheal epithelial cells. |
| topic | Biophysics Biochemistry Microbiology Cell Biology Molecular Biology Biotechnology Evolutionary Biology Infectious Diseases precise molecular role fold organization within intricate &# 8220 xlink "> centrioles radially symmetric 9 molecular hub connecting luminal ring structure c2cd3 localizes close c2cd3 depletion disrupts centriole &# 8217 distal microtubule cap appendages </ p distal appendage assembly &# 8221 shorter centrioles architectural hub appendage defects distal lumen distal end distal centriole like structure protein c2cd3 c2cd3 forms c2cd3 adopts triplet microtubules terminal region polarized microtubule orofaciodigital syndrome ofd1 ), mutations linked mouse tissues human cells extends radially electron tomography disco complex cilia formation centriole interior centriolar lumen based structures also destabilizes 27 nodes |