Filamin A KO and S2152A KI in donor cells do not affect KSHV cell-to-cell viral transmission.
<p>A. Stable Bac16 or STOP45-harboring Filamin WT, S2152A KI or KO HEK293-mCherry cells were induced with TPA + NaB for 72 h, and then cells were collected and washed twice, and directly added to the monolayer of HEK293 cells and co-cultured for additional 24 h. Total cells were harvested and...
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2025
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| 总结: | <p>A. Stable Bac16 or STOP45-harboring Filamin WT, S2152A KI or KO HEK293-mCherry cells were induced with TPA + NaB for 72 h, and then cells were collected and washed twice, and directly added to the monolayer of HEK293 cells and co-cultured for additional 24 h. Total cells were harvested and GFP-positive mCherry-negative cells were analyzed by FACS flow cytometer. The representative images and the percentages of KSHV-infected HEK293 cells were calculated in three independent experiments and shown. ns, no statistical significance; **, p < 0.01; t test.</p> <p>(TIF)</p> |
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