Filamin A KO and S2152A KI in donor cells do not affect KSHV cell-to-cell viral transmission.
<p>A. Stable Bac16 or STOP45-harboring Filamin WT, S2152A KI or KO HEK293-mCherry cells were induced with TPA + NaB for 72 h, and then cells were collected and washed twice, and directly added to the monolayer of HEK293 cells and co-cultured for additional 24 h. Total cells were harvested and...
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2025
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| _version_ | 1849927641664061440 |
|---|---|
| author | Xiaojuan Li (351842) |
| author2 | Li Quan (177024) Rihong Zhou (22676690) Xiangpeng Liu (17898881) Ronit Sarid (186795) Ersheng Kuang (129028) |
| author2_role | author author author author author |
| author_facet | Xiaojuan Li (351842) Li Quan (177024) Rihong Zhou (22676690) Xiangpeng Liu (17898881) Ronit Sarid (186795) Ersheng Kuang (129028) |
| author_role | author |
| dc.creator.none.fl_str_mv | Xiaojuan Li (351842) Li Quan (177024) Rihong Zhou (22676690) Xiangpeng Liu (17898881) Ronit Sarid (186795) Ersheng Kuang (129028) |
| dc.date.none.fl_str_mv | 2025-11-24T18:34:19Z |
| dc.identifier.none.fl_str_mv | 10.1371/journal.ppat.1013737.s005 |
| dc.relation.none.fl_str_mv | https://figshare.com/articles/figure/Filamin_A_KO_and_S2152A_KI_in_donor_cells_do_not_affect_KSHV_cell-to-cell_viral_transmission_/30697688 |
| dc.rights.none.fl_str_mv | CC BY 4.0 info:eu-repo/semantics/openAccess |
| dc.subject.none.fl_str_mv | Microbiology Cell Biology Molecular Biology Biotechnology Immunology Developmental Biology Cancer Infectious Diseases Virology s2152a knockin abolish primary effusion lymphoma multicentric castleman disease kaposi &# 8217 mediated viral infection infected adherent cells differentiated b cells sequential cell detachment mediated kshv transmission kshv lytic replication adherent cells lytic replication viral transmission kshv transmission cell detachment b lymphocytes lytic cycle cell transmission taken together results demonstrated radically support natural donors key role key regulator etiological agent dependent manner cell contact cell adhesion associated herpesvirus |
| dc.title.none.fl_str_mv | Filamin A KO and S2152A KI in donor cells do not affect KSHV cell-to-cell viral transmission. |
| dc.type.none.fl_str_mv | Image Figure info:eu-repo/semantics/publishedVersion image |
| description | <p>A. Stable Bac16 or STOP45-harboring Filamin WT, S2152A KI or KO HEK293-mCherry cells were induced with TPA + NaB for 72 h, and then cells were collected and washed twice, and directly added to the monolayer of HEK293 cells and co-cultured for additional 24 h. Total cells were harvested and GFP-positive mCherry-negative cells were analyzed by FACS flow cytometer. The representative images and the percentages of KSHV-infected HEK293 cells were calculated in three independent experiments and shown. ns, no statistical significance; **, p < 0.01; t test.</p> <p>(TIF)</p> |
| eu_rights_str_mv | openAccess |
| id | Manara_519ebd805548209df614087ebffc49b0 |
| identifier_str_mv | 10.1371/journal.ppat.1013737.s005 |
| network_acronym_str | Manara |
| network_name_str | ManaraRepo |
| oai_identifier_str | oai:figshare.com:article/30697688 |
| publishDate | 2025 |
| repository.mail.fl_str_mv | |
| repository.name.fl_str_mv | |
| repository_id_str | |
| rights_invalid_str_mv | CC BY 4.0 |
| spelling | Filamin A KO and S2152A KI in donor cells do not affect KSHV cell-to-cell viral transmission.Xiaojuan Li (351842)Li Quan (177024)Rihong Zhou (22676690)Xiangpeng Liu (17898881)Ronit Sarid (186795)Ersheng Kuang (129028)MicrobiologyCell BiologyMolecular BiologyBiotechnologyImmunologyDevelopmental BiologyCancerInfectious DiseasesVirologys2152a knockin abolishprimary effusion lymphomamulticentric castleman diseasekaposi &# 8217mediated viral infectioninfected adherent cellsdifferentiated b cellssequential cell detachmentmediated kshv transmissionkshv lytic replicationadherent cellslytic replicationviral transmissionkshv transmissioncell detachmentb lymphocyteslytic cyclecell transmissiontaken togetherresults demonstratedradically supportnatural donorskey rolekey regulatoretiological agentdependent mannercell contactcell adhesionassociated herpesvirus<p>A. Stable Bac16 or STOP45-harboring Filamin WT, S2152A KI or KO HEK293-mCherry cells were induced with TPA + NaB for 72 h, and then cells were collected and washed twice, and directly added to the monolayer of HEK293 cells and co-cultured for additional 24 h. Total cells were harvested and GFP-positive mCherry-negative cells were analyzed by FACS flow cytometer. The representative images and the percentages of KSHV-infected HEK293 cells were calculated in three independent experiments and shown. ns, no statistical significance; **, p < 0.01; t test.</p> <p>(TIF)</p>2025-11-24T18:34:19ZImageFigureinfo:eu-repo/semantics/publishedVersionimage10.1371/journal.ppat.1013737.s005https://figshare.com/articles/figure/Filamin_A_KO_and_S2152A_KI_in_donor_cells_do_not_affect_KSHV_cell-to-cell_viral_transmission_/30697688CC BY 4.0info:eu-repo/semantics/openAccessoai:figshare.com:article/306976882025-11-24T18:34:19Z |
| spellingShingle | Filamin A KO and S2152A KI in donor cells do not affect KSHV cell-to-cell viral transmission. Xiaojuan Li (351842) Microbiology Cell Biology Molecular Biology Biotechnology Immunology Developmental Biology Cancer Infectious Diseases Virology s2152a knockin abolish primary effusion lymphoma multicentric castleman disease kaposi &# 8217 mediated viral infection infected adherent cells differentiated b cells sequential cell detachment mediated kshv transmission kshv lytic replication adherent cells lytic replication viral transmission kshv transmission cell detachment b lymphocytes lytic cycle cell transmission taken together results demonstrated radically support natural donors key role key regulator etiological agent dependent manner cell contact cell adhesion associated herpesvirus |
| status_str | publishedVersion |
| title | Filamin A KO and S2152A KI in donor cells do not affect KSHV cell-to-cell viral transmission. |
| title_full | Filamin A KO and S2152A KI in donor cells do not affect KSHV cell-to-cell viral transmission. |
| title_fullStr | Filamin A KO and S2152A KI in donor cells do not affect KSHV cell-to-cell viral transmission. |
| title_full_unstemmed | Filamin A KO and S2152A KI in donor cells do not affect KSHV cell-to-cell viral transmission. |
| title_short | Filamin A KO and S2152A KI in donor cells do not affect KSHV cell-to-cell viral transmission. |
| title_sort | Filamin A KO and S2152A KI in donor cells do not affect KSHV cell-to-cell viral transmission. |
| topic | Microbiology Cell Biology Molecular Biology Biotechnology Immunology Developmental Biology Cancer Infectious Diseases Virology s2152a knockin abolish primary effusion lymphoma multicentric castleman disease kaposi &# 8217 mediated viral infection infected adherent cells differentiated b cells sequential cell detachment mediated kshv transmission kshv lytic replication adherent cells lytic replication viral transmission kshv transmission cell detachment b lymphocytes lytic cycle cell transmission taken together results demonstrated radically support natural donors key role key regulator etiological agent dependent manner cell contact cell adhesion associated herpesvirus |