Influence of the methanol supply on DNase1 expression.
<p>(A) Raising MeOH above 0.5% (v/v) in the expression medium increases preαMF-mDNase1 production with an optimum at 5% MeOH and 24 °C (p = 0.0059) and even more at 2.5% and 28 °C (p = 0.0441) as determined by HCA of SN. Employing two expression cassettes (2x) led to a delayed rise. (B, C) I...
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2025
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| Summary: | <p>(A) Raising MeOH above 0.5% (v/v) in the expression medium increases preαMF-mDNase1 production with an optimum at 5% MeOH and 24 °C (p = 0.0059) and even more at 2.5% and 28 °C (p = 0.0441) as determined by HCA of SN. Employing two expression cassettes (2x) led to a delayed rise. (B, C) Identical results obtained for preαMF-hDNASE1<sup>co</sup> as illustrated by silver gel analysis of SN combined with DPZ below. (D) Expression of preαMF-hDNASE1<sup>co</sup> at optimal 5% MeOH and 24 °C is slightly lower to 2.5% MeOH and 28 °C as evaluated by DPZ. (E) Comparable to preαMF-mDNase1, two preαMF-hDNASE1<sup>co</sup> transgenes (2x) delay and decrease expression as shown by DPZ. Marker: Prestained protein marker PAN-Biotech™. Data shown for single exemplary experiments using clone 2 and 1 for preαMF-mDNase1 1x and 2x as well as clone 3 and 1 for preαMF-hDNASE1<sup>co</sup> 1x and 2x presented in <a href="http://www.plosone.org/article/info:doi/10.1371/journal.pone.0321094#pone.0321094.g003" target="_blank">Fig 3</a> and <a href="http://www.plosone.org/article/info:doi/10.1371/journal.pone.0321094#pone.0321094.g004" target="_blank">4</a>.</p> |
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