Influence of the methanol supply on DNase1 expression.

<p>(A) Raising MeOH above 0.5% (v/v) in the expression medium increases preαMF-mDNase1 production with an optimum at 5% MeOH and 24 °C (p = 0.0059) and even more at 2.5% and 28 °C (p = 0.0441) as determined by HCA of SN. Employing two expression cassettes (2x) led to a delayed rise. (B, C) I...

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Bibliographic Details
Main Author:	Jan-Ole Krischek (21213122) (author)
Other Authors:	Hans Georg Mannherz (10226546) (author), Markus Napirei (10226549) (author)
Published:	2025
Subjects:	Biochemistry Genetics Molecular Biology Biotechnology Biological Sciences not elsewhere classified Chemical Sciences not elsewhere classified systemic lupus erythematosus rough endoplasmic reticulum related waste disposal major endonuclease involved important therapeutic biologic higher specific activity chinese hamster ovary also detected previously 19 associated microangiopathy saccharomyces cerevisiae </ pichia pastoris </ div >< p using mdnase1 expression recombinant murine dnase1 pastoris </ recombinant dnase1 murine dnase1 translational import since deoxyribonuclease salient feature rhdnase1 produced rhdnase1 expressed rhdnase1 due rhdnase1 ). pulmozyme ®, prolonged persistence orthologues share oligosaccharyltransferase complex native conformation methanol supply main cause lower inhibition improvements resulted impaired cleavage high structural hdnase1 resulted hdnase1 fourfold hdnase1 expression functional homology extracellular chromatin expression difference cultivation temperature concerning functionality comparative expression codon usage cell density biochemical effectivity =&# 8201 24 hours
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Influence of the methanol supply on DNase1 expression.

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