Antibody information used for Western blot.
<div><p>Purpose</p><p>Chronic atrophic gastritis (CAG) is a prevalent condition that can undergo precancerous lesions of gastric cancer (PLGC) and thus progression to gastric cancer. Weifuchun (WFC), a traditional Chinese herbal compound, is employed in CAG treatment and micr...
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2025
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| _version_ | 1849927643755970560 |
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| author | Yingying Chen (500738) |
| author2 | Ping Feng (501575) Xiaoqin Lou (9550704) Haiying Wang (6869) Yanlan Hong (22676265) Jiaojiao Yang (4497643) Shenbao Wu (6075737) |
| author2_role | author author author author author author |
| author_facet | Yingying Chen (500738) Ping Feng (501575) Xiaoqin Lou (9550704) Haiying Wang (6869) Yanlan Hong (22676265) Jiaojiao Yang (4497643) Shenbao Wu (6075737) |
| author_role | author |
| dc.creator.none.fl_str_mv | Yingying Chen (500738) Ping Feng (501575) Xiaoqin Lou (9550704) Haiying Wang (6869) Yanlan Hong (22676265) Jiaojiao Yang (4497643) Shenbao Wu (6075737) |
| dc.date.none.fl_str_mv | 2025-11-24T18:22:08Z |
| dc.identifier.none.fl_str_mv | 10.1371/journal.pone.0333375.t001 |
| dc.relation.none.fl_str_mv | https://figshare.com/articles/dataset/Antibody_information_used_for_Western_blot_/30696384 |
| dc.rights.none.fl_str_mv | CC BY 4.0 info:eu-repo/semantics/openAccess |
| dc.subject.none.fl_str_mv | Biochemistry Medicine Microbiology Cell Biology Genetics Molecular Biology Pharmacology Immunology Cancer Infectious Diseases Chemical Sciences not elsewhere classified undergo precancerous lesions targeted therapeutic mechanism providing novel insights microbial homeostasis modulation janus kinase 1 wfc &# 8217 weifuchun alleviates mnng il )- 6 wfc increased wfc improved wfc compositions wfc ), various doses transcription 3 thus progression significantly correlated signal transducer prevalent condition potential connection pathological changes organic acids nitrosoguanidine treatment key proteins inflammatory markers gastrointestinal microbiota dysplasia caused coprocuccus </ compounds identified atrophic gastritis aforementioned results |
| dc.title.none.fl_str_mv | Antibody information used for Western blot. |
| dc.type.none.fl_str_mv | Dataset info:eu-repo/semantics/publishedVersion dataset |
| description | <div><p>Purpose</p><p>Chronic atrophic gastritis (CAG) is a prevalent condition that can undergo precancerous lesions of gastric cancer (PLGC) and thus progression to gastric cancer. Weifuchun (WFC), a traditional Chinese herbal compound, is employed in CAG treatment and microbial homeostasis modulation. We aimed to explore the role of WFC in CAG from the perspective of gastrointestinal microbiota.</p><p>Methods</p><p>PLGC rats were developed by N-methyl-N’-nitro-N-nitrosoguanidine treatment. Then, various doses of WFC were administered to the rats and WFC compositions were analyzed. The pathological changes and inflammatory markers of the rats were assessed. Key proteins in the interleukin (IL)-6/Janus Kinase 1/Signal Transducer and Activator of Transcription 3 (JAK1/STAT3) pathway were evaluated, alongside fecal microbial analysis through 16S ribosomal DNA sequencing.</p><p>Results</p><p>Most compounds identified from WFC were nucleosides, flavones, organic acids, phenylpropanoids and other components. WFC treatment alleviated intestinal metaplasia and dysplasia caused by atrophic gastritis in PLGC rats. WFC also reduced inflammatory marker levels, and inhibited the IL-6/JAK1/STAT3 pathway for PLGC rats. The higher the dose of WFC was administrated, the better the effects on the aforementioned results. Simultaneously, WFC improved the CAG gastrointestinal microbiota homeostasis. Interestingly, WFC increased <i>Coprocuccus</i> abundance, which was significantly correlated with proliferating cell nuclear antigen and IL-6 levels.</p><p>Conclusions</p><p>WFC alleviated PLGC by regulating the balance of gastrointestinal microbiota and influencing the IL-6/JAK1/STAT3 pathway, revealing the potential connection between gastrointestinal microbiota and CAG progression. This study is the first to link gastrointestinal microbiota homeostasis and IL-6/JAK1/STAT3 pathway, providing novel insights into WFC’s multi-targeted therapeutic mechanism on CAG.</p></div> |
| eu_rights_str_mv | openAccess |
| id | Manara_5506f07e378d0e94e3743b7d721f0af6 |
| identifier_str_mv | 10.1371/journal.pone.0333375.t001 |
| network_acronym_str | Manara |
| network_name_str | ManaraRepo |
| oai_identifier_str | oai:figshare.com:article/30696384 |
| publishDate | 2025 |
| repository.mail.fl_str_mv | |
| repository.name.fl_str_mv | |
| repository_id_str | |
| rights_invalid_str_mv | CC BY 4.0 |
| spelling | Antibody information used for Western blot.Yingying Chen (500738)Ping Feng (501575)Xiaoqin Lou (9550704)Haiying Wang (6869)Yanlan Hong (22676265)Jiaojiao Yang (4497643)Shenbao Wu (6075737)BiochemistryMedicineMicrobiologyCell BiologyGeneticsMolecular BiologyPharmacologyImmunologyCancerInfectious DiseasesChemical Sciences not elsewhere classifiedundergo precancerous lesionstargeted therapeutic mechanismproviding novel insightsmicrobial homeostasis modulationjanus kinase 1wfc &# 8217weifuchun alleviates mnngil )- 6wfc increasedwfc improvedwfc compositionswfc ),various dosestranscription 3thus progressionsignificantly correlatedsignal transducerprevalent conditionpotential connectionpathological changesorganic acidsnitrosoguanidine treatmentkey proteinsinflammatory markersgastrointestinal microbiotadysplasia causedcoprocuccus </compounds identifiedatrophic gastritisaforementioned results<div><p>Purpose</p><p>Chronic atrophic gastritis (CAG) is a prevalent condition that can undergo precancerous lesions of gastric cancer (PLGC) and thus progression to gastric cancer. Weifuchun (WFC), a traditional Chinese herbal compound, is employed in CAG treatment and microbial homeostasis modulation. We aimed to explore the role of WFC in CAG from the perspective of gastrointestinal microbiota.</p><p>Methods</p><p>PLGC rats were developed by N-methyl-N’-nitro-N-nitrosoguanidine treatment. Then, various doses of WFC were administered to the rats and WFC compositions were analyzed. The pathological changes and inflammatory markers of the rats were assessed. Key proteins in the interleukin (IL)-6/Janus Kinase 1/Signal Transducer and Activator of Transcription 3 (JAK1/STAT3) pathway were evaluated, alongside fecal microbial analysis through 16S ribosomal DNA sequencing.</p><p>Results</p><p>Most compounds identified from WFC were nucleosides, flavones, organic acids, phenylpropanoids and other components. WFC treatment alleviated intestinal metaplasia and dysplasia caused by atrophic gastritis in PLGC rats. WFC also reduced inflammatory marker levels, and inhibited the IL-6/JAK1/STAT3 pathway for PLGC rats. The higher the dose of WFC was administrated, the better the effects on the aforementioned results. Simultaneously, WFC improved the CAG gastrointestinal microbiota homeostasis. Interestingly, WFC increased <i>Coprocuccus</i> abundance, which was significantly correlated with proliferating cell nuclear antigen and IL-6 levels.</p><p>Conclusions</p><p>WFC alleviated PLGC by regulating the balance of gastrointestinal microbiota and influencing the IL-6/JAK1/STAT3 pathway, revealing the potential connection between gastrointestinal microbiota and CAG progression. This study is the first to link gastrointestinal microbiota homeostasis and IL-6/JAK1/STAT3 pathway, providing novel insights into WFC’s multi-targeted therapeutic mechanism on CAG.</p></div>2025-11-24T18:22:08ZDatasetinfo:eu-repo/semantics/publishedVersiondataset10.1371/journal.pone.0333375.t001https://figshare.com/articles/dataset/Antibody_information_used_for_Western_blot_/30696384CC BY 4.0info:eu-repo/semantics/openAccessoai:figshare.com:article/306963842025-11-24T18:22:08Z |
| spellingShingle | Antibody information used for Western blot. Yingying Chen (500738) Biochemistry Medicine Microbiology Cell Biology Genetics Molecular Biology Pharmacology Immunology Cancer Infectious Diseases Chemical Sciences not elsewhere classified undergo precancerous lesions targeted therapeutic mechanism providing novel insights microbial homeostasis modulation janus kinase 1 wfc &# 8217 weifuchun alleviates mnng il )- 6 wfc increased wfc improved wfc compositions wfc ), various doses transcription 3 thus progression significantly correlated signal transducer prevalent condition potential connection pathological changes organic acids nitrosoguanidine treatment key proteins inflammatory markers gastrointestinal microbiota dysplasia caused coprocuccus </ compounds identified atrophic gastritis aforementioned results |
| status_str | publishedVersion |
| title | Antibody information used for Western blot. |
| title_full | Antibody information used for Western blot. |
| title_fullStr | Antibody information used for Western blot. |
| title_full_unstemmed | Antibody information used for Western blot. |
| title_short | Antibody information used for Western blot. |
| title_sort | Antibody information used for Western blot. |
| topic | Biochemistry Medicine Microbiology Cell Biology Genetics Molecular Biology Pharmacology Immunology Cancer Infectious Diseases Chemical Sciences not elsewhere classified undergo precancerous lesions targeted therapeutic mechanism providing novel insights microbial homeostasis modulation janus kinase 1 wfc &# 8217 weifuchun alleviates mnng il )- 6 wfc increased wfc improved wfc compositions wfc ), various doses transcription 3 thus progression significantly correlated signal transducer prevalent condition potential connection pathological changes organic acids nitrosoguanidine treatment key proteins inflammatory markers gastrointestinal microbiota dysplasia caused coprocuccus </ compounds identified atrophic gastritis aforementioned results |