Electrochemical Calcite-Assisted Localization and Kinetics (E-CLocK) Microscopy
Nanoparticle (NP) morphology is a critical factor influencing the efficiency and selectivity of electrochemical reactions. However, conventional electrochemical techniques do not provide information about the dynamic morphological changes of NPs during these reactions. Advanced methods such as atomi...
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2025
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| _version_ | 1852019388169846784 |
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| author | Ivani Jayalath (21526945) |
| author2 | Shubhendra Shukla (14259106) Govinda Anantha Padmanabha (21526948) Vignesh Sundaresan (3591446) |
| author2_role | author author author |
| author_facet | Ivani Jayalath (21526945) Shubhendra Shukla (14259106) Govinda Anantha Padmanabha (21526948) Vignesh Sundaresan (3591446) |
| author_role | author |
| dc.creator.none.fl_str_mv | Ivani Jayalath (21526945) Shubhendra Shukla (14259106) Govinda Anantha Padmanabha (21526948) Vignesh Sundaresan (3591446) |
| dc.date.none.fl_str_mv | 2025-06-11T18:34:24Z |
| dc.identifier.none.fl_str_mv | 10.1021/acs.analchem.5c02072.s001 |
| dc.relation.none.fl_str_mv | https://figshare.com/articles/media/Electrochemical_Calcite-Assisted_Localization_and_Kinetics_E-CLocK_Microscopy/29297986 |
| dc.rights.none.fl_str_mv | CC BY-NC 4.0 info:eu-repo/semantics/openAccess |
| dc.subject.none.fl_str_mv | Biophysics Molecular Biology Physiology Biotechnology Ecology Developmental Biology Plant Biology Biological Sciences not elsewhere classified Chemical Sciences not elsewhere classified offer higher resolution novel multiparameter super induced oscillatory behavior gold precursor solution critical factor influencing quantitative morphological changes dynamic morphological changes rotating calcite crystal conventional electrochemical techniques transmission electron microscopy atomic force microscopy tracking morphological changes based electrochemical calcite significantly advancing single clock microscopy provides electrochemical calcite significantly promoted invasive tracking electrochemical processes microscopy nanoparticle clock microscopy morphological anisotropy reliable method quantitatively assessed provide information nanoparticle level model reaction infinity space ctab ), cetyltrimethylammonium bromide assisted localization advanced methods |
| dc.title.none.fl_str_mv | Electrochemical Calcite-Assisted Localization and Kinetics (E-CLocK) Microscopy |
| dc.type.none.fl_str_mv | Dataset Media info:eu-repo/semantics/publishedVersion dataset |
| description | Nanoparticle (NP) morphology is a critical factor influencing the efficiency and selectivity of electrochemical reactions. However, conventional electrochemical techniques do not provide information about the dynamic morphological changes of NPs during these reactions. Advanced methods such as atomic force microscopy (AFM) and transmission electron microscopy (TEM) offer higher resolution but are costly and may impact electrochemical measurements. Here, we introduce dark-field-based electrochemical calcite-assisted localization and kinetics (E-CLocK) microscopy, a novel multiparameter super-resolution imaging technique enabling real-time, non-invasive tracking of qualitative and quantitative morphological changes at the single-nanoparticle level during electrochemical processes. In E-CLocK microscopy, a rotating calcite crystal is integrated into the infinity space of a dark-field microscope, generating a distinctive point spread function that can be analyzed to determine the anisotropy and orientation of NPs. Using gold NP electrodeposition as a model reaction, we quantitatively assessed the morphological anisotropy of the individual NPs during their growth. Nearly all particles exhibited steady isotropic growth with only the gold precursor solution; however, the addition of cetyltrimethylammonium bromide (CTAB), a surfactant, induced oscillatory behavior and significantly promoted the growth of anisotropic NPs. E-CLocK microscopy provides a high-throughput and reliable method for tracking morphological changes during electrochemical reactions, significantly advancing single-particle structure–activity studies. |
| eu_rights_str_mv | openAccess |
| id | Manara_5573fb03ee652eddf52bcdca30de47d1 |
| identifier_str_mv | 10.1021/acs.analchem.5c02072.s001 |
| network_acronym_str | Manara |
| network_name_str | ManaraRepo |
| oai_identifier_str | oai:figshare.com:article/29297986 |
| publishDate | 2025 |
| repository.mail.fl_str_mv | |
| repository.name.fl_str_mv | |
| repository_id_str | |
| rights_invalid_str_mv | CC BY-NC 4.0 |
| spelling | Electrochemical Calcite-Assisted Localization and Kinetics (E-CLocK) MicroscopyIvani Jayalath (21526945)Shubhendra Shukla (14259106)Govinda Anantha Padmanabha (21526948)Vignesh Sundaresan (3591446)BiophysicsMolecular BiologyPhysiologyBiotechnologyEcologyDevelopmental BiologyPlant BiologyBiological Sciences not elsewhere classifiedChemical Sciences not elsewhere classifiedoffer higher resolutionnovel multiparameter superinduced oscillatory behaviorgold precursor solutioncritical factor influencingquantitative morphological changesdynamic morphological changesrotating calcite crystalconventional electrochemical techniquestransmission electron microscopyatomic force microscopytracking morphological changesbased electrochemical calcitesignificantly advancing singleclock microscopy provideselectrochemical calcitesignificantly promotedinvasive trackingelectrochemical processesmicroscopy nanoparticleclock microscopymorphological anisotropyreliable methodquantitatively assessedprovide informationnanoparticle levelmodel reactioninfinity spacectab ),cetyltrimethylammonium bromideassisted localizationadvanced methodsNanoparticle (NP) morphology is a critical factor influencing the efficiency and selectivity of electrochemical reactions. However, conventional electrochemical techniques do not provide information about the dynamic morphological changes of NPs during these reactions. Advanced methods such as atomic force microscopy (AFM) and transmission electron microscopy (TEM) offer higher resolution but are costly and may impact electrochemical measurements. Here, we introduce dark-field-based electrochemical calcite-assisted localization and kinetics (E-CLocK) microscopy, a novel multiparameter super-resolution imaging technique enabling real-time, non-invasive tracking of qualitative and quantitative morphological changes at the single-nanoparticle level during electrochemical processes. In E-CLocK microscopy, a rotating calcite crystal is integrated into the infinity space of a dark-field microscope, generating a distinctive point spread function that can be analyzed to determine the anisotropy and orientation of NPs. Using gold NP electrodeposition as a model reaction, we quantitatively assessed the morphological anisotropy of the individual NPs during their growth. Nearly all particles exhibited steady isotropic growth with only the gold precursor solution; however, the addition of cetyltrimethylammonium bromide (CTAB), a surfactant, induced oscillatory behavior and significantly promoted the growth of anisotropic NPs. E-CLocK microscopy provides a high-throughput and reliable method for tracking morphological changes during electrochemical reactions, significantly advancing single-particle structure–activity studies.2025-06-11T18:34:24ZDatasetMediainfo:eu-repo/semantics/publishedVersiondataset10.1021/acs.analchem.5c02072.s001https://figshare.com/articles/media/Electrochemical_Calcite-Assisted_Localization_and_Kinetics_E-CLocK_Microscopy/29297986CC BY-NC 4.0info:eu-repo/semantics/openAccessoai:figshare.com:article/292979862025-06-11T18:34:24Z |
| spellingShingle | Electrochemical Calcite-Assisted Localization and Kinetics (E-CLocK) Microscopy Ivani Jayalath (21526945) Biophysics Molecular Biology Physiology Biotechnology Ecology Developmental Biology Plant Biology Biological Sciences not elsewhere classified Chemical Sciences not elsewhere classified offer higher resolution novel multiparameter super induced oscillatory behavior gold precursor solution critical factor influencing quantitative morphological changes dynamic morphological changes rotating calcite crystal conventional electrochemical techniques transmission electron microscopy atomic force microscopy tracking morphological changes based electrochemical calcite significantly advancing single clock microscopy provides electrochemical calcite significantly promoted invasive tracking electrochemical processes microscopy nanoparticle clock microscopy morphological anisotropy reliable method quantitatively assessed provide information nanoparticle level model reaction infinity space ctab ), cetyltrimethylammonium bromide assisted localization advanced methods |
| status_str | publishedVersion |
| title | Electrochemical Calcite-Assisted Localization and Kinetics (E-CLocK) Microscopy |
| title_full | Electrochemical Calcite-Assisted Localization and Kinetics (E-CLocK) Microscopy |
| title_fullStr | Electrochemical Calcite-Assisted Localization and Kinetics (E-CLocK) Microscopy |
| title_full_unstemmed | Electrochemical Calcite-Assisted Localization and Kinetics (E-CLocK) Microscopy |
| title_short | Electrochemical Calcite-Assisted Localization and Kinetics (E-CLocK) Microscopy |
| title_sort | Electrochemical Calcite-Assisted Localization and Kinetics (E-CLocK) Microscopy |
| topic | Biophysics Molecular Biology Physiology Biotechnology Ecology Developmental Biology Plant Biology Biological Sciences not elsewhere classified Chemical Sciences not elsewhere classified offer higher resolution novel multiparameter super induced oscillatory behavior gold precursor solution critical factor influencing quantitative morphological changes dynamic morphological changes rotating calcite crystal conventional electrochemical techniques transmission electron microscopy atomic force microscopy tracking morphological changes based electrochemical calcite significantly advancing single clock microscopy provides electrochemical calcite significantly promoted invasive tracking electrochemical processes microscopy nanoparticle clock microscopy morphological anisotropy reliable method quantitatively assessed provide information nanoparticle level model reaction infinity space ctab ), cetyltrimethylammonium bromide assisted localization advanced methods |