Image 2_Immune-related RELT drives clear cell renal cell carcinoma progression through JAK/STAT signaling pathway activation.tiff
Objective<p>The experiment aims to verify the function of Tumor Necrosis Factor Receptor Superfamily Member 19L (RELT) in clear cell renal cell carcinoma (ccRCC).</p>Methods<p>The relationship between differential expression of RELT in ccRCC and clinical prognosis was investigated...
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2025
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| _version_ | 1849927636614119424 |
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| author | Yini Wang (410389) |
| author2 | Yingying Yang (1696258) Tianqi Wang (4093009) Xiaohong Ma (462719) Jitao Wu (695795) |
| author2_role | author author author author |
| author_facet | Yini Wang (410389) Yingying Yang (1696258) Tianqi Wang (4093009) Xiaohong Ma (462719) Jitao Wu (695795) |
| author_role | author |
| dc.creator.none.fl_str_mv | Yini Wang (410389) Yingying Yang (1696258) Tianqi Wang (4093009) Xiaohong Ma (462719) Jitao Wu (695795) |
| dc.date.none.fl_str_mv | 2025-11-25T06:15:31Z |
| dc.identifier.none.fl_str_mv | 10.3389/fimmu.2025.1659119.s001 |
| dc.relation.none.fl_str_mv | https://figshare.com/articles/figure/Image_2_Immune-related_RELT_drives_clear_cell_renal_cell_carcinoma_progression_through_JAK_STAT_signaling_pathway_activation_tiff/30703676 |
| dc.rights.none.fl_str_mv | CC BY 4.0 info:eu-repo/semantics/openAccess |
| dc.subject.none.fl_str_mv | Genetic Immunology ccRCC RELT tumor immune microenvironment JAK/STAT pathway T cell immunity |
| dc.title.none.fl_str_mv | Image 2_Immune-related RELT drives clear cell renal cell carcinoma progression through JAK/STAT signaling pathway activation.tiff |
| dc.type.none.fl_str_mv | Image Figure info:eu-repo/semantics/publishedVersion image |
| description | Objective<p>The experiment aims to verify the function of Tumor Necrosis Factor Receptor Superfamily Member 19L (RELT) in clear cell renal cell carcinoma (ccRCC).</p>Methods<p>The relationship between differential expression of RELT in ccRCC and clinical prognosis was investigated based on data from the Gene Expression Omnibus database (GEO) and The Cancer Genome Atlas (TCGA) databases. Ex vivo and in vivo experiments were applied to validate the function of RELT in ccRCC. The pathways through which RELT exerts its function were explored using analyses such as Gene Ontology (GO), Kyoto Encyclopedia of Genes and Genomes Enrichment Analysis (KEGG), and Gene Set Enrichment Analysis (GSEA). In addition, we applied the algorithms xCELL, Estimating the Proportion of Immune and Cancer cells (EPIC), CIBERSORT, Tumor Immune Estimation Resource (TIMER), and Tumor Immune Dysfunction and Exclusion (TIDE) to analyze the effect of RELT on the ccRCC tumor immune microenvironment.</p>Results<p>RELT is highly expressed in ccRCC tissues and portends poor prognosis. Functional assays indicate that RELT promotes malignant biological behavior in ccRCC cells. Subsequently, enrichment analysis revealed that RELT functions mainly through humoral immunity, cellular chemotaxis, and cytokine regulation and may serve as a molecule for predicting prognosis in ccRCC. Immune infiltration analysis showed that RELT was significantly associated with immune cells such as B Cells, CD8+ T Cells, CD4+ T Cells, and Macrophages and may affect the tumor immune microenvironment of ccRCC by influencing macrophages.</p>Conclusion<p>RELT promotes the development of ccRCC and may play a role in regulating the tumor immune microenvironment, which affects the prognosis of ccRCC patients, and RELT may become a new biomarker associated with immune infiltration in ccRCC.</p> |
| eu_rights_str_mv | openAccess |
| id | Manara_5668e07985a9336e486d9fe6f2db1311 |
| identifier_str_mv | 10.3389/fimmu.2025.1659119.s001 |
| network_acronym_str | Manara |
| network_name_str | ManaraRepo |
| oai_identifier_str | oai:figshare.com:article/30703676 |
| publishDate | 2025 |
| repository.mail.fl_str_mv | |
| repository.name.fl_str_mv | |
| repository_id_str | |
| rights_invalid_str_mv | CC BY 4.0 |
| spelling | Image 2_Immune-related RELT drives clear cell renal cell carcinoma progression through JAK/STAT signaling pathway activation.tiffYini Wang (410389)Yingying Yang (1696258)Tianqi Wang (4093009)Xiaohong Ma (462719)Jitao Wu (695795)Genetic ImmunologyccRCCRELTtumor immune microenvironmentJAK/STAT pathwayT cell immunityObjective<p>The experiment aims to verify the function of Tumor Necrosis Factor Receptor Superfamily Member 19L (RELT) in clear cell renal cell carcinoma (ccRCC).</p>Methods<p>The relationship between differential expression of RELT in ccRCC and clinical prognosis was investigated based on data from the Gene Expression Omnibus database (GEO) and The Cancer Genome Atlas (TCGA) databases. Ex vivo and in vivo experiments were applied to validate the function of RELT in ccRCC. The pathways through which RELT exerts its function were explored using analyses such as Gene Ontology (GO), Kyoto Encyclopedia of Genes and Genomes Enrichment Analysis (KEGG), and Gene Set Enrichment Analysis (GSEA). In addition, we applied the algorithms xCELL, Estimating the Proportion of Immune and Cancer cells (EPIC), CIBERSORT, Tumor Immune Estimation Resource (TIMER), and Tumor Immune Dysfunction and Exclusion (TIDE) to analyze the effect of RELT on the ccRCC tumor immune microenvironment.</p>Results<p>RELT is highly expressed in ccRCC tissues and portends poor prognosis. Functional assays indicate that RELT promotes malignant biological behavior in ccRCC cells. Subsequently, enrichment analysis revealed that RELT functions mainly through humoral immunity, cellular chemotaxis, and cytokine regulation and may serve as a molecule for predicting prognosis in ccRCC. Immune infiltration analysis showed that RELT was significantly associated with immune cells such as B Cells, CD8+ T Cells, CD4+ T Cells, and Macrophages and may affect the tumor immune microenvironment of ccRCC by influencing macrophages.</p>Conclusion<p>RELT promotes the development of ccRCC and may play a role in regulating the tumor immune microenvironment, which affects the prognosis of ccRCC patients, and RELT may become a new biomarker associated with immune infiltration in ccRCC.</p>2025-11-25T06:15:31ZImageFigureinfo:eu-repo/semantics/publishedVersionimage10.3389/fimmu.2025.1659119.s001https://figshare.com/articles/figure/Image_2_Immune-related_RELT_drives_clear_cell_renal_cell_carcinoma_progression_through_JAK_STAT_signaling_pathway_activation_tiff/30703676CC BY 4.0info:eu-repo/semantics/openAccessoai:figshare.com:article/307036762025-11-25T06:15:31Z |
| spellingShingle | Image 2_Immune-related RELT drives clear cell renal cell carcinoma progression through JAK/STAT signaling pathway activation.tiff Yini Wang (410389) Genetic Immunology ccRCC RELT tumor immune microenvironment JAK/STAT pathway T cell immunity |
| status_str | publishedVersion |
| title | Image 2_Immune-related RELT drives clear cell renal cell carcinoma progression through JAK/STAT signaling pathway activation.tiff |
| title_full | Image 2_Immune-related RELT drives clear cell renal cell carcinoma progression through JAK/STAT signaling pathway activation.tiff |
| title_fullStr | Image 2_Immune-related RELT drives clear cell renal cell carcinoma progression through JAK/STAT signaling pathway activation.tiff |
| title_full_unstemmed | Image 2_Immune-related RELT drives clear cell renal cell carcinoma progression through JAK/STAT signaling pathway activation.tiff |
| title_short | Image 2_Immune-related RELT drives clear cell renal cell carcinoma progression through JAK/STAT signaling pathway activation.tiff |
| title_sort | Image 2_Immune-related RELT drives clear cell renal cell carcinoma progression through JAK/STAT signaling pathway activation.tiff |
| topic | Genetic Immunology ccRCC RELT tumor immune microenvironment JAK/STAT pathway T cell immunity |