BTB domain and CNC homolog 1 (BACH1) is stabilized by Polo-like kinase 1 (PLK1).
<p><b>(A and B)</b> BACH1 protein level was detected in mouse (A) and human (B) melanoma cells under the treatment of either DMSO, Nocodazole (NOC), or NOC plus Volasertib. <b>(C and D)</b> Immunoblots of BACH1 protein level in the mouse (C) and human (D) melanoma cells...
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2025
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| _version_ | 1849927642779746304 |
|---|---|
| author | Fengyi Mao (15073242) |
| author2 | Sai Wu (6096290) Derek B. Allison (15141675) Daheng He (9189701) Yifan Kong (4458616) Chaohao Li (15141681) Zhiguo Li (431917) Yanquan Zhang (14930570) Xinyi Wang (559030) Qiongsi Zhang (2818111) Chi Wang (131921) Xiaoqi Liu (45630) |
| author2_role | author author author author author author author author author author author |
| author_facet | Fengyi Mao (15073242) Sai Wu (6096290) Derek B. Allison (15141675) Daheng He (9189701) Yifan Kong (4458616) Chaohao Li (15141681) Zhiguo Li (431917) Yanquan Zhang (14930570) Xinyi Wang (559030) Qiongsi Zhang (2818111) Chi Wang (131921) Xiaoqi Liu (45630) |
| author_role | author |
| dc.creator.none.fl_str_mv | Fengyi Mao (15073242) Sai Wu (6096290) Derek B. Allison (15141675) Daheng He (9189701) Yifan Kong (4458616) Chaohao Li (15141681) Zhiguo Li (431917) Yanquan Zhang (14930570) Xinyi Wang (559030) Qiongsi Zhang (2818111) Chi Wang (131921) Xiaoqi Liu (45630) |
| dc.date.none.fl_str_mv | 2025-11-24T18:28:16Z |
| dc.identifier.none.fl_str_mv | 10.1371/journal.pbio.3003490.g005 |
| dc.relation.none.fl_str_mv | https://figshare.com/articles/figure/BTB_domain_and_CNC_homolog_1_BACH1_is_stabilized_by_Polo-like_kinase_1_PLK1_/30697058 |
| dc.rights.none.fl_str_mv | CC BY 4.0 info:eu-repo/semantics/openAccess |
| dc.subject.none.fl_str_mv | Cell Biology Genetics Molecular Biology Developmental Biology Cancer Hematology Biological Sciences not elsewhere classified suppress tumor growth like kinase 1 crucial transcription factor cnc homolog 1 clinical database analysis shortened mice survival >< sup >< div >< p cutaneous melanoma based v600e </ sup patient survival pten </ ca </ ></ sup synergistic efficiency novel function negatively correlates genes involved cell cycle cancer progression cancer metabolism bach1 ), >- deficiency |
| dc.title.none.fl_str_mv | BTB domain and CNC homolog 1 (BACH1) is stabilized by Polo-like kinase 1 (PLK1). |
| dc.type.none.fl_str_mv | Image Figure info:eu-repo/semantics/publishedVersion image |
| description | <p><b>(A and B)</b> BACH1 protein level was detected in mouse (A) and human (B) melanoma cells under the treatment of either DMSO, Nocodazole (NOC), or NOC plus Volasertib. <b>(C and D)</b> Immunoblots of BACH1 protein level in the mouse (C) and human (D) melanoma cells under the treatment of Cycloheximide (CHX) at indicated time points with or without Volasertib. <b>(E)</b> Human melanoma cells were treated with Hemin and collected at the indicated time points, followed by the immunoblots to determine the BACH1 protein. <b>(F)</b> [<sup>35</sup>S]-methionine pulse chase assay was performed in the A375R cells. (<b>G and H</b>) mRNA expression level of GAPDH and HK2 was analyzed by qRT-PCR in mouse (G) and human (H) melanoma cells. Mean ± SD. n.s., <i>P</i> > 0.05; * <i>P</i> < 0.05; ** <i>P</i> < 0.01 by unpaired Student <i>t</i> test. <i>n</i> = 3 biological replicates. <b>(I and J)</b> Chromatin-immunoprecipitation (ChIP) was performed to detect the chromatin occupancy of BACH1 in mouse (I) and human (J) melanoma cells. Mean ± SD. n.s., <i>P</i> > 0.05; * <i>P</i> < 0.05; ** <i>P</i> < 0.01 by unpaired Student <i>t</i> test. <i>n</i> = 3 biological replicates. <b>(K)</b> A working model to illustrate how PLK1 promotes melanoma progression through BACH1. PLK1 could prevent BACH1 from proteasome degradation, resulting in hyper-activation of BACH1. Consequently, the downstream targets of BACH1 were regulated accordingly, thus promoting metabolic reprogramming and metastasis in melanoma. This figure was created with BioRender.com. The data underlying the graphs shown in the figure can be found in <a href="http://www.plosbiology.org/article/info:doi/10.1371/journal.pbio.3003490#pbio.3003490.s014" target="_blank">S1 Data</a>.</p> |
| eu_rights_str_mv | openAccess |
| id | Manara_5696529626f0520a78d831daf5a90fa6 |
| identifier_str_mv | 10.1371/journal.pbio.3003490.g005 |
| network_acronym_str | Manara |
| network_name_str | ManaraRepo |
| oai_identifier_str | oai:figshare.com:article/30697058 |
| publishDate | 2025 |
| repository.mail.fl_str_mv | |
| repository.name.fl_str_mv | |
| repository_id_str | |
| rights_invalid_str_mv | CC BY 4.0 |
| spelling | BTB domain and CNC homolog 1 (BACH1) is stabilized by Polo-like kinase 1 (PLK1).Fengyi Mao (15073242)Sai Wu (6096290)Derek B. Allison (15141675)Daheng He (9189701)Yifan Kong (4458616)Chaohao Li (15141681)Zhiguo Li (431917)Yanquan Zhang (14930570)Xinyi Wang (559030)Qiongsi Zhang (2818111)Chi Wang (131921)Xiaoqi Liu (45630)Cell BiologyGeneticsMolecular BiologyDevelopmental BiologyCancerHematologyBiological Sciences not elsewhere classifiedsuppress tumor growthlike kinase 1crucial transcription factorcnc homolog 1clinical database analysisshortened mice survival>< sup ><div >< pcutaneous melanoma basedv600e </ suppatient survivalpten </ca </></ supsynergistic efficiencynovel functionnegatively correlatesgenes involvedcell cyclecancer progressioncancer metabolismbach1 ),>- deficiency<p><b>(A and B)</b> BACH1 protein level was detected in mouse (A) and human (B) melanoma cells under the treatment of either DMSO, Nocodazole (NOC), or NOC plus Volasertib. <b>(C and D)</b> Immunoblots of BACH1 protein level in the mouse (C) and human (D) melanoma cells under the treatment of Cycloheximide (CHX) at indicated time points with or without Volasertib. <b>(E)</b> Human melanoma cells were treated with Hemin and collected at the indicated time points, followed by the immunoblots to determine the BACH1 protein. <b>(F)</b> [<sup>35</sup>S]-methionine pulse chase assay was performed in the A375R cells. (<b>G and H</b>) mRNA expression level of GAPDH and HK2 was analyzed by qRT-PCR in mouse (G) and human (H) melanoma cells. Mean ± SD. n.s., <i>P</i> > 0.05; * <i>P</i> < 0.05; ** <i>P</i> < 0.01 by unpaired Student <i>t</i> test. <i>n</i> = 3 biological replicates. <b>(I and J)</b> Chromatin-immunoprecipitation (ChIP) was performed to detect the chromatin occupancy of BACH1 in mouse (I) and human (J) melanoma cells. Mean ± SD. n.s., <i>P</i> > 0.05; * <i>P</i> < 0.05; ** <i>P</i> < 0.01 by unpaired Student <i>t</i> test. <i>n</i> = 3 biological replicates. <b>(K)</b> A working model to illustrate how PLK1 promotes melanoma progression through BACH1. PLK1 could prevent BACH1 from proteasome degradation, resulting in hyper-activation of BACH1. Consequently, the downstream targets of BACH1 were regulated accordingly, thus promoting metabolic reprogramming and metastasis in melanoma. This figure was created with BioRender.com. The data underlying the graphs shown in the figure can be found in <a href="http://www.plosbiology.org/article/info:doi/10.1371/journal.pbio.3003490#pbio.3003490.s014" target="_blank">S1 Data</a>.</p>2025-11-24T18:28:16ZImageFigureinfo:eu-repo/semantics/publishedVersionimage10.1371/journal.pbio.3003490.g005https://figshare.com/articles/figure/BTB_domain_and_CNC_homolog_1_BACH1_is_stabilized_by_Polo-like_kinase_1_PLK1_/30697058CC BY 4.0info:eu-repo/semantics/openAccessoai:figshare.com:article/306970582025-11-24T18:28:16Z |
| spellingShingle | BTB domain and CNC homolog 1 (BACH1) is stabilized by Polo-like kinase 1 (PLK1). Fengyi Mao (15073242) Cell Biology Genetics Molecular Biology Developmental Biology Cancer Hematology Biological Sciences not elsewhere classified suppress tumor growth like kinase 1 crucial transcription factor cnc homolog 1 clinical database analysis shortened mice survival >< sup >< div >< p cutaneous melanoma based v600e </ sup patient survival pten </ ca </ ></ sup synergistic efficiency novel function negatively correlates genes involved cell cycle cancer progression cancer metabolism bach1 ), >- deficiency |
| status_str | publishedVersion |
| title | BTB domain and CNC homolog 1 (BACH1) is stabilized by Polo-like kinase 1 (PLK1). |
| title_full | BTB domain and CNC homolog 1 (BACH1) is stabilized by Polo-like kinase 1 (PLK1). |
| title_fullStr | BTB domain and CNC homolog 1 (BACH1) is stabilized by Polo-like kinase 1 (PLK1). |
| title_full_unstemmed | BTB domain and CNC homolog 1 (BACH1) is stabilized by Polo-like kinase 1 (PLK1). |
| title_short | BTB domain and CNC homolog 1 (BACH1) is stabilized by Polo-like kinase 1 (PLK1). |
| title_sort | BTB domain and CNC homolog 1 (BACH1) is stabilized by Polo-like kinase 1 (PLK1). |
| topic | Cell Biology Genetics Molecular Biology Developmental Biology Cancer Hematology Biological Sciences not elsewhere classified suppress tumor growth like kinase 1 crucial transcription factor cnc homolog 1 clinical database analysis shortened mice survival >< sup >< div >< p cutaneous melanoma based v600e </ sup patient survival pten </ ca </ ></ sup synergistic efficiency novel function negatively correlates genes involved cell cycle cancer progression cancer metabolism bach1 ), >- deficiency |