Changes in myofibroblast markers and matrix molecule mRNA expression in isolated cardiac fibroblasts after AngII stimulation and PAR2 inhibition.
<p>Cardiac fibroblasts isolated from neonatal rats were treated with anti-PAR2 blocking antibody SAM11 (αPAR2, n = 5 each) and 2μM PAR2 inhibitor AZ3541 (PAR2 I., n = 3 each) or left untreated (0, n = 5 each) or treated with isotype control antibody (iso, n = 3) as a control using unstimulated...
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2024
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| Summary: | <p>Cardiac fibroblasts isolated from neonatal rats were treated with anti-PAR2 blocking antibody SAM11 (αPAR2, n = 5 each) and 2μM PAR2 inhibitor AZ3541 (PAR2 I., n = 3 each) or left untreated (0, n = 5 each) or treated with isotype control antibody (iso, n = 3) as a control using unstimulated or AngII stimulated cells (hatched bars). After lysis of fibroblasts mRNA levels of myofibroblast markers <b>A:</b> <i>Acta2</i> (SMA), <b>B:</b> <i>Col1a1</i> (collagen 1), matrix molecules <b>C:</b> <i>Col4a1</i> (collagen 4) and <b>D:</b> <i>Fn1</i> (fibronectin) and the pro-fibrotic cytokine <b>E:</b> <i>Tgfb1</i> (TGF-ß) were measured. Significant changes compared to untreated control (0) were marked: *p<0.05; **p<0.01.</p> |
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