Localization of the CTC to the mitochondrion of <i>Toxoplasma gondii</i> is mediated by <i>Tg</i>CIA1.
<p><b>(A–D)</b> Immunofluorescence assays of <b>(A)</b> r<i>Tg</i>CIA1-mAID-cMyc/<i>Tg</i>CIA2-HA, <b>(B)</b> r<i>Tg</i>CIA1-mAID-cMyc/<i>Tg</i>MMS19-HA, <b>(C)</b> r<i>Tg</i>CIA2-mAID-H...
সংরক্ষণ করুন:
| প্রধান লেখক: | |
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| অন্যান্য লেখক: | , , , |
| প্রকাশিত: |
2025
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| বিষয়গুলি: | |
| ট্যাগগুলো: |
ট্যাগ যুক্ত করুন
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| সংক্ষিপ্ত: | <p><b>(A–D)</b> Immunofluorescence assays of <b>(A)</b> r<i>Tg</i>CIA1-mAID-cMyc/<i>Tg</i>CIA2-HA, <b>(B)</b> r<i>Tg</i>CIA1-mAID-cMyc/<i>Tg</i>MMS19-HA, <b>(C)</b> r<i>Tg</i>CIA2-mAID-HA/<i>Tg</i>CIA1-smFP-cMyc, and <b>(D)</b> r<i>Tg</i>MMS19-mAID-HA/<i>Tg</i>CIA1-smFP-cMyc parasites, cultured in the absence (top) or presence (bottom) of IAA for either 3 h <b>(A, B)</b> or 24 h (C, D). Samples were probed with anti-HA <b>(A, B)</b> or anti-cMyc <b>(C, D)</b> antibodies to detect the protein-of-interest (green) and anti-<i>Tg</i>Tom40 antibodies to detect the mitochondrion (magenta). Scale bars are 2 µm. DIC, differential interference contrast. Middle, corresponding fluorescence profiles depicting the intensity of anti-HA or anti-cMyc labeling (green) and the anti-<i>Tg</i>Tom40 labeling (magenta) along the yellow line on merged images. Right, the correlation between the protein of interest and <i>Tg</i>Tom40 was quantified using the Pearson correlation coefficient (<i>r</i>) and analyzed using an unpaired <i>t</i> test, with the <i>p</i> values shown. The numerical data underlying this Figure can be found in <a href="http://www.plosbiology.org/article/info:doi/10.1371/journal.pbio.3003520#pbio.3003520.s016" target="_blank">S1 Data</a>.</p> |
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