Localization of the CTC to the mitochondrion of <i>Toxoplasma gondii</i> is mediated by <i>Tg</i>CIA1.
<p><b>(A–D)</b> Immunofluorescence assays of <b>(A)</b> r<i>Tg</i>CIA1-mAID-cMyc/<i>Tg</i>CIA2-HA, <b>(B)</b> r<i>Tg</i>CIA1-mAID-cMyc/<i>Tg</i>MMS19-HA, <b>(C)</b> r<i>Tg</i>CIA2-mAID-H...
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| مؤلفون آخرون: | , , , |
| منشور في: |
2025
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| _version_ | 1849927626941005824 |
|---|---|
| author | Evie R. Hodgson (21866946) |
| author2 | Jenni A. Hayward (10063377) Rachel A. Leonard (5195411) F. Victor Makota (16625292) Giel G. van Dooren (10063383) |
| author2_role | author author author author |
| author_facet | Evie R. Hodgson (21866946) Jenni A. Hayward (10063377) Rachel A. Leonard (5195411) F. Victor Makota (16625292) Giel G. van Dooren (10063383) |
| author_role | author |
| dc.creator.none.fl_str_mv | Evie R. Hodgson (21866946) Jenni A. Hayward (10063377) Rachel A. Leonard (5195411) F. Victor Makota (16625292) Giel G. van Dooren (10063383) |
| dc.date.none.fl_str_mv | 2025-11-25T18:38:25Z |
| dc.identifier.none.fl_str_mv | 10.1371/journal.pbio.3003520.g005 |
| dc.relation.none.fl_str_mv | https://figshare.com/articles/figure/Localization_of_the_CTC_to_the_mitochondrion_of_i_Toxoplasma_gondii_i_is_mediated_by_i_Tg_i_CIA1_/30714643 |
| dc.rights.none.fl_str_mv | CC BY 4.0 info:eu-repo/semantics/openAccess |
| dc.subject.none.fl_str_mv | Biochemistry Medicine Microbiology Cell Biology Immunology Infectious Diseases Environmental Sciences not elsewhere classified Biological Sciences not elsewhere classified Physical Sciences not elsewhere classified iron – sulfur eukaryotic cell biology elucidates pivotal differences div >< p exhibit dual localization nuclear client proteins toxoplasma gondii </ cia targeting complex toxoplasma </ nuclear proteins gondii </ mitochondrial targeting three proteins study provides showed previously scaffold protein remained unclear present evidence otherwise ancient may reflect living relatives important group functionally conserved comprehensive analysis closest free cia1 protein |
| dc.title.none.fl_str_mv | Localization of the CTC to the mitochondrion of <i>Toxoplasma gondii</i> is mediated by <i>Tg</i>CIA1. |
| dc.type.none.fl_str_mv | Image Figure info:eu-repo/semantics/publishedVersion image |
| description | <p><b>(A–D)</b> Immunofluorescence assays of <b>(A)</b> r<i>Tg</i>CIA1-mAID-cMyc/<i>Tg</i>CIA2-HA, <b>(B)</b> r<i>Tg</i>CIA1-mAID-cMyc/<i>Tg</i>MMS19-HA, <b>(C)</b> r<i>Tg</i>CIA2-mAID-HA/<i>Tg</i>CIA1-smFP-cMyc, and <b>(D)</b> r<i>Tg</i>MMS19-mAID-HA/<i>Tg</i>CIA1-smFP-cMyc parasites, cultured in the absence (top) or presence (bottom) of IAA for either 3 h <b>(A, B)</b> or 24 h (C, D). Samples were probed with anti-HA <b>(A, B)</b> or anti-cMyc <b>(C, D)</b> antibodies to detect the protein-of-interest (green) and anti-<i>Tg</i>Tom40 antibodies to detect the mitochondrion (magenta). Scale bars are 2 µm. DIC, differential interference contrast. Middle, corresponding fluorescence profiles depicting the intensity of anti-HA or anti-cMyc labeling (green) and the anti-<i>Tg</i>Tom40 labeling (magenta) along the yellow line on merged images. Right, the correlation between the protein of interest and <i>Tg</i>Tom40 was quantified using the Pearson correlation coefficient (<i>r</i>) and analyzed using an unpaired <i>t</i> test, with the <i>p</i> values shown. The numerical data underlying this Figure can be found in <a href="http://www.plosbiology.org/article/info:doi/10.1371/journal.pbio.3003520#pbio.3003520.s016" target="_blank">S1 Data</a>.</p> |
| eu_rights_str_mv | openAccess |
| id | Manara_5f43f96150d74850c2bff518ded3d62d |
| identifier_str_mv | 10.1371/journal.pbio.3003520.g005 |
| network_acronym_str | Manara |
| network_name_str | ManaraRepo |
| oai_identifier_str | oai:figshare.com:article/30714643 |
| publishDate | 2025 |
| repository.mail.fl_str_mv | |
| repository.name.fl_str_mv | |
| repository_id_str | |
| rights_invalid_str_mv | CC BY 4.0 |
| spelling | Localization of the CTC to the mitochondrion of <i>Toxoplasma gondii</i> is mediated by <i>Tg</i>CIA1.Evie R. Hodgson (21866946)Jenni A. Hayward (10063377)Rachel A. Leonard (5195411)F. Victor Makota (16625292)Giel G. van Dooren (10063383)BiochemistryMedicineMicrobiologyCell BiologyImmunologyInfectious DiseasesEnvironmental Sciences not elsewhere classifiedBiological Sciences not elsewhere classifiedPhysical Sciences not elsewhere classifiediron – sulfureukaryotic cell biologyelucidates pivotal differencesdiv >< pexhibit dual localizationnuclear client proteinstoxoplasma gondii </cia targeting complextoxoplasma </nuclear proteinsgondii </mitochondrial targetingthree proteinsstudy providesshowed previouslyscaffold proteinremained unclearpresent evidenceotherwise ancientmay reflectliving relativesimportant groupfunctionally conservedcomprehensive analysisclosest freecia1 protein<p><b>(A–D)</b> Immunofluorescence assays of <b>(A)</b> r<i>Tg</i>CIA1-mAID-cMyc/<i>Tg</i>CIA2-HA, <b>(B)</b> r<i>Tg</i>CIA1-mAID-cMyc/<i>Tg</i>MMS19-HA, <b>(C)</b> r<i>Tg</i>CIA2-mAID-HA/<i>Tg</i>CIA1-smFP-cMyc, and <b>(D)</b> r<i>Tg</i>MMS19-mAID-HA/<i>Tg</i>CIA1-smFP-cMyc parasites, cultured in the absence (top) or presence (bottom) of IAA for either 3 h <b>(A, B)</b> or 24 h (C, D). Samples were probed with anti-HA <b>(A, B)</b> or anti-cMyc <b>(C, D)</b> antibodies to detect the protein-of-interest (green) and anti-<i>Tg</i>Tom40 antibodies to detect the mitochondrion (magenta). Scale bars are 2 µm. DIC, differential interference contrast. Middle, corresponding fluorescence profiles depicting the intensity of anti-HA or anti-cMyc labeling (green) and the anti-<i>Tg</i>Tom40 labeling (magenta) along the yellow line on merged images. Right, the correlation between the protein of interest and <i>Tg</i>Tom40 was quantified using the Pearson correlation coefficient (<i>r</i>) and analyzed using an unpaired <i>t</i> test, with the <i>p</i> values shown. The numerical data underlying this Figure can be found in <a href="http://www.plosbiology.org/article/info:doi/10.1371/journal.pbio.3003520#pbio.3003520.s016" target="_blank">S1 Data</a>.</p>2025-11-25T18:38:25ZImageFigureinfo:eu-repo/semantics/publishedVersionimage10.1371/journal.pbio.3003520.g005https://figshare.com/articles/figure/Localization_of_the_CTC_to_the_mitochondrion_of_i_Toxoplasma_gondii_i_is_mediated_by_i_Tg_i_CIA1_/30714643CC BY 4.0info:eu-repo/semantics/openAccessoai:figshare.com:article/307146432025-11-25T18:38:25Z |
| spellingShingle | Localization of the CTC to the mitochondrion of <i>Toxoplasma gondii</i> is mediated by <i>Tg</i>CIA1. Evie R. Hodgson (21866946) Biochemistry Medicine Microbiology Cell Biology Immunology Infectious Diseases Environmental Sciences not elsewhere classified Biological Sciences not elsewhere classified Physical Sciences not elsewhere classified iron – sulfur eukaryotic cell biology elucidates pivotal differences div >< p exhibit dual localization nuclear client proteins toxoplasma gondii </ cia targeting complex toxoplasma </ nuclear proteins gondii </ mitochondrial targeting three proteins study provides showed previously scaffold protein remained unclear present evidence otherwise ancient may reflect living relatives important group functionally conserved comprehensive analysis closest free cia1 protein |
| status_str | publishedVersion |
| title | Localization of the CTC to the mitochondrion of <i>Toxoplasma gondii</i> is mediated by <i>Tg</i>CIA1. |
| title_full | Localization of the CTC to the mitochondrion of <i>Toxoplasma gondii</i> is mediated by <i>Tg</i>CIA1. |
| title_fullStr | Localization of the CTC to the mitochondrion of <i>Toxoplasma gondii</i> is mediated by <i>Tg</i>CIA1. |
| title_full_unstemmed | Localization of the CTC to the mitochondrion of <i>Toxoplasma gondii</i> is mediated by <i>Tg</i>CIA1. |
| title_short | Localization of the CTC to the mitochondrion of <i>Toxoplasma gondii</i> is mediated by <i>Tg</i>CIA1. |
| title_sort | Localization of the CTC to the mitochondrion of <i>Toxoplasma gondii</i> is mediated by <i>Tg</i>CIA1. |
| topic | Biochemistry Medicine Microbiology Cell Biology Immunology Infectious Diseases Environmental Sciences not elsewhere classified Biological Sciences not elsewhere classified Physical Sciences not elsewhere classified iron – sulfur eukaryotic cell biology elucidates pivotal differences div >< p exhibit dual localization nuclear client proteins toxoplasma gondii </ cia targeting complex toxoplasma </ nuclear proteins gondii </ mitochondrial targeting three proteins study provides showed previously scaffold protein remained unclear present evidence otherwise ancient may reflect living relatives important group functionally conserved comprehensive analysis closest free cia1 protein |