Supplementary Material 3
<p dir="ltr">BUSCO (Benchmarking Universal Single-Copy Orthologs) is a bioinformatics tool used to assess the completeness of genome assemblies, transcriptomes, and gene sets by evaluating the presence of conserved single-copy orthologs across evolutionary lineages. It quantitatively...
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2025
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| Summary: | <p dir="ltr">BUSCO (Benchmarking Universal Single-Copy Orthologs) is a bioinformatics tool used to assess the completeness of genome assemblies, transcriptomes, and gene sets by evaluating the presence of conserved single-copy orthologs across evolutionary lineages. It quantitatively measures genome quality by classifying genes into complete, duplicated, fragmented, or missing categories. This tool is vital in microbial genomics, as it helps researchers determine the integrity of assembled genomes, ensuring that essential genes are present and minimizing assembly errors. By comparing the genomic content against well-curated lineage-specific databases, BUSCO aids in quality control, phylogenetic analysis, and evolutionary studies, making it a crucial tool for genome annotation and comparative genomics.</p> |
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