Phenotypic consequences of the conoid detachment.

(A) U-ExM images of CPH1 (conoid marker) and APR1 (APR marker) without or without the conoid detached from the APR. Scale bar = 3 μm. (B) U-ExM of secretory organelles, with MIC2 used as a microneme marker and RON9 used as a rhoptry neck marker. Images...

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প্রধান লেখক:	Romuald Haase (22339777) (author)
অন্যান্য লেখক:	Bingjian Ren (22676577) (author), Albert Tell i Puig (22676580) (author), Alessandro Bonavoglia (19415718) (author), Jean-Baptiste Marq (375239) (author), Rémy Visentin (22676583) (author), Nicolas Dos Santos Pacheco (22676586) (author), Bohumil Maco (383443) (author), Ricardo Mondragón-Flores (10737057) (author), Oscar Vadas (2416228) (author), Dominique Soldati-Favre (79026) (author)
প্রকাশিত:	2025
বিষয়গুলি:	Biophysics Biochemistry Microbiology Cell Biology Physiology Infectious Diseases Environmental Sciences not elsewhere classified Biological Sciences not elsewhere classified Physical Sciences not elsewhere classified toxoplasma gondii </ remains apically anchored heterologous expression systems div >< p apical polar ring biochemical analysis revealed proper conoid anchorage ensures conoid functionality less parasites revealed host cell invasion enable parasite motility controlling parasite motility although rng2 depletion intact conoid organelle organelle traverses intact protein conditional depletion biochemical properties detached conoid gliding motility unstable protein undergoes extrusion two structures strong candidate striking detachment still unclear rng2 tethers rng2 led rng2 emerges rhoptries follow resilient bridge protein localized plasma membrane pivotal protein likely facilitate large coiled immunoelectron microscopy functional activity enabling rng2 electron tomography dependent extrusion critical role concatenated assemblies comprehensive mutagenesis coil domains calcium waves
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বিবরন
সংক্ষিপ্ত:	<p><b>(A)</b> U-ExM images of CPH1 (conoid marker) and APR1 (APR marker) without or without the conoid detached from the APR. Scale bar = 3 μm. <b>(B)</b> U-ExM of secretory organelles, with MIC2 used as a microneme marker and RON9 used as a rhoptry neck marker. Images taken with the conoid attached or detached from the APR. Scale bar = 3 μm <b>(C)</b> Western blot analysis of ethanol-triggered microneme secretion. ESA, Excreted secreted antigens. <b>(D)</b>. Representative images of BIPPO triggered egress. Scale bar = 20 μm. <b>(E)</b> Left panel: representative images of STAT6-P-positive cells with a Tir1 strain. Right panel: Quantification of rhoptry secretion was performed to assess STAT-6 phosphorylation. At least 200 cell nuclei were counted for data plotting, <i>n</i> = 3. ATc = anhydrotetracycline. The data underlying this figure can be found in <a href="http://www.plosbiology.org/article/info:doi/10.1371/journal.pbio.3003506#pbio.3003506.s008" target="_blank">S2 Data</a>.</p>

Phenotypic consequences of the conoid detachment.

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