Additional information on the RNG2 variants expressed in UPRT locus.
<p><b>(A)</b> IFA localization and plaque assay assessment of the first round of RNG2 variants. Cell marker: IMC1. Scale bar = 2 μm. <b>(B)</b> IFA localization and plaque assay assessment of the second round of the RNG2 variant. Cell marker: GAP45. Scale bar = 2 μm <...
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| _version_ | 1849927642243923968 |
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| author | Romuald Haase (22339777) |
| author2 | Bingjian Ren (22676577) Albert Tell i Puig (22676580) Alessandro Bonavoglia (19415718) Jean-Baptiste Marq (375239) Rémy Visentin (22676583) Nicolas Dos Santos Pacheco (22676586) Bohumil Maco (383443) Ricardo Mondragón-Flores (10737057) Oscar Vadas (2416228) Dominique Soldati-Favre (79026) |
| author2_role | author author author author author author author author author author |
| author_facet | Romuald Haase (22339777) Bingjian Ren (22676577) Albert Tell i Puig (22676580) Alessandro Bonavoglia (19415718) Jean-Baptiste Marq (375239) Rémy Visentin (22676583) Nicolas Dos Santos Pacheco (22676586) Bohumil Maco (383443) Ricardo Mondragón-Flores (10737057) Oscar Vadas (2416228) Dominique Soldati-Favre (79026) |
| author_role | author |
| dc.creator.none.fl_str_mv | Romuald Haase (22339777) Bingjian Ren (22676577) Albert Tell i Puig (22676580) Alessandro Bonavoglia (19415718) Jean-Baptiste Marq (375239) Rémy Visentin (22676583) Nicolas Dos Santos Pacheco (22676586) Bohumil Maco (383443) Ricardo Mondragón-Flores (10737057) Oscar Vadas (2416228) Dominique Soldati-Favre (79026) |
| dc.date.none.fl_str_mv | 2025-11-24T18:30:54Z |
| dc.identifier.none.fl_str_mv | 10.1371/journal.pbio.3003506.s006 |
| dc.relation.none.fl_str_mv | https://figshare.com/articles/figure/Additional_information_on_the_RNG2_variants_expressed_in_UPRT_locus_/30697329 |
| dc.rights.none.fl_str_mv | CC BY 4.0 info:eu-repo/semantics/openAccess |
| dc.subject.none.fl_str_mv | Biophysics Biochemistry Microbiology Cell Biology Physiology Infectious Diseases Environmental Sciences not elsewhere classified Biological Sciences not elsewhere classified Physical Sciences not elsewhere classified toxoplasma gondii </ remains apically anchored heterologous expression systems div >< p apical polar ring biochemical analysis revealed proper conoid anchorage ensures conoid functionality less parasites revealed host cell invasion enable parasite motility controlling parasite motility although rng2 depletion intact conoid organelle organelle traverses intact protein conditional depletion biochemical properties detached conoid gliding motility unstable protein undergoes extrusion two structures strong candidate striking detachment still unclear rng2 tethers rng2 led rng2 emerges rhoptries follow resilient bridge protein localized plasma membrane pivotal protein likely facilitate large coiled immunoelectron microscopy functional activity enabling rng2 electron tomography dependent extrusion critical role concatenated assemblies comprehensive mutagenesis coil domains calcium waves |
| dc.title.none.fl_str_mv | Additional information on the RNG2 variants expressed in UPRT locus. |
| dc.type.none.fl_str_mv | Image Figure info:eu-repo/semantics/publishedVersion image |
| description | <p><b>(A)</b> IFA localization and plaque assay assessment of the first round of RNG2 variants. Cell marker: IMC1. Scale bar = 2 μm. <b>(B)</b> IFA localization and plaque assay assessment of the second round of the RNG2 variant. Cell marker: GAP45. Scale bar = 2 μm <b>(C)</b> IFA localization and plaque assay assessment of the processed forms observed in insect cells and expressed in the UPRT locus. Cell marker: GAP45. Scale bar = 2 μm.</p> <p>(TIFF)</p> |
| eu_rights_str_mv | openAccess |
| id | Manara_6792d4ed2a2afd444b79a6846e851341 |
| identifier_str_mv | 10.1371/journal.pbio.3003506.s006 |
| network_acronym_str | Manara |
| network_name_str | ManaraRepo |
| oai_identifier_str | oai:figshare.com:article/30697329 |
| publishDate | 2025 |
| repository.mail.fl_str_mv | |
| repository.name.fl_str_mv | |
| repository_id_str | |
| rights_invalid_str_mv | CC BY 4.0 |
| spelling | Additional information on the RNG2 variants expressed in UPRT locus.Romuald Haase (22339777)Bingjian Ren (22676577)Albert Tell i Puig (22676580)Alessandro Bonavoglia (19415718)Jean-Baptiste Marq (375239)Rémy Visentin (22676583)Nicolas Dos Santos Pacheco (22676586)Bohumil Maco (383443)Ricardo Mondragón-Flores (10737057)Oscar Vadas (2416228)Dominique Soldati-Favre (79026)BiophysicsBiochemistryMicrobiologyCell BiologyPhysiologyInfectious DiseasesEnvironmental Sciences not elsewhere classifiedBiological Sciences not elsewhere classifiedPhysical Sciences not elsewhere classifiedtoxoplasma gondii </remains apically anchoredheterologous expression systemsdiv >< papical polar ringbiochemical analysis revealedproper conoid anchorageensures conoid functionalityless parasites revealedhost cell invasionenable parasite motilitycontrolling parasite motilityalthough rng2 depletionintact conoid organelleorganelle traversesintact proteinconditional depletionbiochemical propertiesdetached conoidgliding motilityunstable proteinundergoes extrusiontwo structuresstrong candidatestriking detachmentstill unclearrng2 tethersrng2 ledrng2 emergesrhoptries followresilient bridgeprotein localizedplasma membranepivotal proteinlikely facilitatelarge coiledimmunoelectron microscopyfunctional activityenabling rng2electron tomographydependent extrusioncritical roleconcatenated assembliescomprehensive mutagenesiscoil domainscalcium waves<p><b>(A)</b> IFA localization and plaque assay assessment of the first round of RNG2 variants. Cell marker: IMC1. Scale bar = 2 μm. <b>(B)</b> IFA localization and plaque assay assessment of the second round of the RNG2 variant. Cell marker: GAP45. Scale bar = 2 μm <b>(C)</b> IFA localization and plaque assay assessment of the processed forms observed in insect cells and expressed in the UPRT locus. Cell marker: GAP45. Scale bar = 2 μm.</p> <p>(TIFF)</p>2025-11-24T18:30:54ZImageFigureinfo:eu-repo/semantics/publishedVersionimage10.1371/journal.pbio.3003506.s006https://figshare.com/articles/figure/Additional_information_on_the_RNG2_variants_expressed_in_UPRT_locus_/30697329CC BY 4.0info:eu-repo/semantics/openAccessoai:figshare.com:article/306973292025-11-24T18:30:54Z |
| spellingShingle | Additional information on the RNG2 variants expressed in UPRT locus. Romuald Haase (22339777) Biophysics Biochemistry Microbiology Cell Biology Physiology Infectious Diseases Environmental Sciences not elsewhere classified Biological Sciences not elsewhere classified Physical Sciences not elsewhere classified toxoplasma gondii </ remains apically anchored heterologous expression systems div >< p apical polar ring biochemical analysis revealed proper conoid anchorage ensures conoid functionality less parasites revealed host cell invasion enable parasite motility controlling parasite motility although rng2 depletion intact conoid organelle organelle traverses intact protein conditional depletion biochemical properties detached conoid gliding motility unstable protein undergoes extrusion two structures strong candidate striking detachment still unclear rng2 tethers rng2 led rng2 emerges rhoptries follow resilient bridge protein localized plasma membrane pivotal protein likely facilitate large coiled immunoelectron microscopy functional activity enabling rng2 electron tomography dependent extrusion critical role concatenated assemblies comprehensive mutagenesis coil domains calcium waves |
| status_str | publishedVersion |
| title | Additional information on the RNG2 variants expressed in UPRT locus. |
| title_full | Additional information on the RNG2 variants expressed in UPRT locus. |
| title_fullStr | Additional information on the RNG2 variants expressed in UPRT locus. |
| title_full_unstemmed | Additional information on the RNG2 variants expressed in UPRT locus. |
| title_short | Additional information on the RNG2 variants expressed in UPRT locus. |
| title_sort | Additional information on the RNG2 variants expressed in UPRT locus. |
| topic | Biophysics Biochemistry Microbiology Cell Biology Physiology Infectious Diseases Environmental Sciences not elsewhere classified Biological Sciences not elsewhere classified Physical Sciences not elsewhere classified toxoplasma gondii </ remains apically anchored heterologous expression systems div >< p apical polar ring biochemical analysis revealed proper conoid anchorage ensures conoid functionality less parasites revealed host cell invasion enable parasite motility controlling parasite motility although rng2 depletion intact conoid organelle organelle traverses intact protein conditional depletion biochemical properties detached conoid gliding motility unstable protein undergoes extrusion two structures strong candidate striking detachment still unclear rng2 tethers rng2 led rng2 emerges rhoptries follow resilient bridge protein localized plasma membrane pivotal protein likely facilitate large coiled immunoelectron microscopy functional activity enabling rng2 electron tomography dependent extrusion critical role concatenated assemblies comprehensive mutagenesis coil domains calcium waves |