Supplementary Figure S5 from Serologic Profiling Using an Epstein-Barr Virus Mammalian Expression Library Identifies EBNA1 IgA as a Prediagnostic Marker for Nasopharyngeal Carcinoma
<p>Serum IgA detection of viral antigens from reactivated HH514-16 lysates. Sera from 38 SCHS and SCS validation pairs (0<10 years to diagnosis and EBNA1 IgA false-positives) were used to probe cell lysates prepared by sodium butyrate reactivation of HH514-16 cells. (A) Example of immunoblo...
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2025
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| _version_ | 1849927633761992704 |
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| author | Sarita Paudel (15047430) |
| author2 | Benjamin E. Warner (15047433) Renwei Wang (15047436) Jennifer Adams-Haduch (15047439) Alex S. Reznik (15047442) Jason Dou (15047445) Yufei Huang (10791191) Yu-Tang Gao (15047448) Woon-Puay Koh (15047451) Alan Bäckerholm (15047454) Jian-Min Yuan (15047457) Kathy H.Y. Shair (15047460) |
| author2_role | author author author author author author author author author author author |
| author_facet | Sarita Paudel (15047430) Benjamin E. Warner (15047433) Renwei Wang (15047436) Jennifer Adams-Haduch (15047439) Alex S. Reznik (15047442) Jason Dou (15047445) Yufei Huang (10791191) Yu-Tang Gao (15047448) Woon-Puay Koh (15047451) Alan Bäckerholm (15047454) Jian-Min Yuan (15047457) Kathy H.Y. Shair (15047460) |
| author_role | author |
| dc.creator.none.fl_str_mv | Sarita Paudel (15047430) Benjamin E. Warner (15047433) Renwei Wang (15047436) Jennifer Adams-Haduch (15047439) Alex S. Reznik (15047442) Jason Dou (15047445) Yufei Huang (10791191) Yu-Tang Gao (15047448) Woon-Puay Koh (15047451) Alan Bäckerholm (15047454) Jian-Min Yuan (15047457) Kathy H.Y. Shair (15047460) |
| dc.date.none.fl_str_mv | 2025-11-25T12:49:01Z |
| dc.identifier.none.fl_str_mv | 10.1158/1078-0432.30706508 |
| dc.relation.none.fl_str_mv | https://figshare.com/articles/figure/Supplementary_Figure_S5_from_Serologic_Profiling_Using_an_Epstein-Barr_Virus_Mammalian_Expression_Library_Identifies_EBNA1_IgA_as_a_Prediagnostic_Marker_for_Nasopharyngeal_Carcinoma/30706508 |
| dc.rights.none.fl_str_mv | CC BY info:eu-repo/semantics/openAccess |
| dc.subject.none.fl_str_mv | Cancer Cancer Biology Cancer Detection and Diagnosis Therapeutic Research and Development Biomarkers Cancer risk biomarkers Serum biomarkers Head, Neck & Oral Cancers Translational Research Viral Oncogenesis Human tumor viruses |
| dc.title.none.fl_str_mv | Supplementary Figure S5 from Serologic Profiling Using an Epstein-Barr Virus Mammalian Expression Library Identifies EBNA1 IgA as a Prediagnostic Marker for Nasopharyngeal Carcinoma |
| dc.type.none.fl_str_mv | Image Figure info:eu-repo/semantics/publishedVersion image |
| description | <p>Serum IgA detection of viral antigens from reactivated HH514-16 lysates. Sera from 38 SCHS and SCS validation pairs (0<10 years to diagnosis and EBNA1 IgA false-positives) were used to probe cell lysates prepared by sodium butyrate reactivation of HH514-16 cells. (A) Example of immunoblot detection of induced (I) and uninduced (U) HH514-16 lysates by sera from NPC case-control pairs within 3 years to diagnosis (NPC45, C45) and >3 years (NPC30, C30) with band calls shown as red dots. Estimated molecular weight (MW) in kilodaltons (kDa) and protein band locations of several early EBV proteins are shown (EA p138 (BALF2), EBNA1 (BKRF1), TK (BXLF1), DNase (BGLF5), EA-D (BMRF1), VCA p40 (BdRF1), ZEBRA (BZLF1), VCA p18 (BFRF3)) (13). EA-D can be multiple bands ranging 47-54 kDa. (B) The number of protein bands detected (band count) are shown for cases (black triangle) and controls (grey inverted triangle) which are grouped by number of years to NPC case diagnosis (years to NPC dx). NPC cases within 2 years to diagnosis (blue triangle) and controls that tested positive for EBNA1 IgA (filled grey inverted triangle). are identified. Median=red bar, n=number of sera in each group. Statistical significance is labeled as *p<0.05, **p<0.001, calculated by a Mann-Whitney test.</p> |
| eu_rights_str_mv | openAccess |
| id | Manara_68dce8f4324f09d5d6af635e63740dd1 |
| identifier_str_mv | 10.1158/1078-0432.30706508 |
| network_acronym_str | Manara |
| network_name_str | ManaraRepo |
| oai_identifier_str | oai:figshare.com:article/30706508 |
| publishDate | 2025 |
| repository.mail.fl_str_mv | |
| repository.name.fl_str_mv | |
| repository_id_str | |
| rights_invalid_str_mv | CC BY |
| spelling | Supplementary Figure S5 from Serologic Profiling Using an Epstein-Barr Virus Mammalian Expression Library Identifies EBNA1 IgA as a Prediagnostic Marker for Nasopharyngeal CarcinomaSarita Paudel (15047430)Benjamin E. Warner (15047433)Renwei Wang (15047436)Jennifer Adams-Haduch (15047439)Alex S. Reznik (15047442)Jason Dou (15047445)Yufei Huang (10791191)Yu-Tang Gao (15047448)Woon-Puay Koh (15047451)Alan Bäckerholm (15047454)Jian-Min Yuan (15047457)Kathy H.Y. Shair (15047460)CancerCancer BiologyCancer Detection and DiagnosisTherapeutic Research and DevelopmentBiomarkersCancer risk biomarkersSerum biomarkersHead, Neck & Oral CancersTranslational ResearchViral OncogenesisHuman tumor viruses<p>Serum IgA detection of viral antigens from reactivated HH514-16 lysates. Sera from 38 SCHS and SCS validation pairs (0<10 years to diagnosis and EBNA1 IgA false-positives) were used to probe cell lysates prepared by sodium butyrate reactivation of HH514-16 cells. (A) Example of immunoblot detection of induced (I) and uninduced (U) HH514-16 lysates by sera from NPC case-control pairs within 3 years to diagnosis (NPC45, C45) and >3 years (NPC30, C30) with band calls shown as red dots. Estimated molecular weight (MW) in kilodaltons (kDa) and protein band locations of several early EBV proteins are shown (EA p138 (BALF2), EBNA1 (BKRF1), TK (BXLF1), DNase (BGLF5), EA-D (BMRF1), VCA p40 (BdRF1), ZEBRA (BZLF1), VCA p18 (BFRF3)) (13). EA-D can be multiple bands ranging 47-54 kDa. (B) The number of protein bands detected (band count) are shown for cases (black triangle) and controls (grey inverted triangle) which are grouped by number of years to NPC case diagnosis (years to NPC dx). NPC cases within 2 years to diagnosis (blue triangle) and controls that tested positive for EBNA1 IgA (filled grey inverted triangle). are identified. Median=red bar, n=number of sera in each group. Statistical significance is labeled as *p<0.05, **p<0.001, calculated by a Mann-Whitney test.</p>2025-11-25T12:49:01ZImageFigureinfo:eu-repo/semantics/publishedVersionimage10.1158/1078-0432.30706508https://figshare.com/articles/figure/Supplementary_Figure_S5_from_Serologic_Profiling_Using_an_Epstein-Barr_Virus_Mammalian_Expression_Library_Identifies_EBNA1_IgA_as_a_Prediagnostic_Marker_for_Nasopharyngeal_Carcinoma/30706508CC BYinfo:eu-repo/semantics/openAccessoai:figshare.com:article/307065082025-11-25T12:49:01Z |
| spellingShingle | Supplementary Figure S5 from Serologic Profiling Using an Epstein-Barr Virus Mammalian Expression Library Identifies EBNA1 IgA as a Prediagnostic Marker for Nasopharyngeal Carcinoma Sarita Paudel (15047430) Cancer Cancer Biology Cancer Detection and Diagnosis Therapeutic Research and Development Biomarkers Cancer risk biomarkers Serum biomarkers Head, Neck & Oral Cancers Translational Research Viral Oncogenesis Human tumor viruses |
| status_str | publishedVersion |
| title | Supplementary Figure S5 from Serologic Profiling Using an Epstein-Barr Virus Mammalian Expression Library Identifies EBNA1 IgA as a Prediagnostic Marker for Nasopharyngeal Carcinoma |
| title_full | Supplementary Figure S5 from Serologic Profiling Using an Epstein-Barr Virus Mammalian Expression Library Identifies EBNA1 IgA as a Prediagnostic Marker for Nasopharyngeal Carcinoma |
| title_fullStr | Supplementary Figure S5 from Serologic Profiling Using an Epstein-Barr Virus Mammalian Expression Library Identifies EBNA1 IgA as a Prediagnostic Marker for Nasopharyngeal Carcinoma |
| title_full_unstemmed | Supplementary Figure S5 from Serologic Profiling Using an Epstein-Barr Virus Mammalian Expression Library Identifies EBNA1 IgA as a Prediagnostic Marker for Nasopharyngeal Carcinoma |
| title_short | Supplementary Figure S5 from Serologic Profiling Using an Epstein-Barr Virus Mammalian Expression Library Identifies EBNA1 IgA as a Prediagnostic Marker for Nasopharyngeal Carcinoma |
| title_sort | Supplementary Figure S5 from Serologic Profiling Using an Epstein-Barr Virus Mammalian Expression Library Identifies EBNA1 IgA as a Prediagnostic Marker for Nasopharyngeal Carcinoma |
| topic | Cancer Cancer Biology Cancer Detection and Diagnosis Therapeutic Research and Development Biomarkers Cancer risk biomarkers Serum biomarkers Head, Neck & Oral Cancers Translational Research Viral Oncogenesis Human tumor viruses |