Image 10_The role of TMEM59L in colorectal cancer progression and its interaction with the TGF-β/Smad pathway.jpeg
Objective<p>This study aimed to investigate the role of TMEM59L in colorectal cancer (CRC) and its interaction with the TGF-β/Smad signaling pathway.</p>Methods<p>We analyzed the correlation between TMEM59L expression levels and patient survival, as well as its impact on the TGF-β/...
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2025
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| _version_ | 1849927636652916736 |
|---|---|
| author | Hongjie Yang (383059) |
| author2 | Jiafei Liu (7301984) Peishi Jiang (10110097) Yi Sun (118759) Lingyi Chen (796170) Siwei Zhu (400113) |
| author2_role | author author author author author |
| author_facet | Hongjie Yang (383059) Jiafei Liu (7301984) Peishi Jiang (10110097) Yi Sun (118759) Lingyi Chen (796170) Siwei Zhu (400113) |
| author_role | author |
| dc.creator.none.fl_str_mv | Hongjie Yang (383059) Jiafei Liu (7301984) Peishi Jiang (10110097) Yi Sun (118759) Lingyi Chen (796170) Siwei Zhu (400113) |
| dc.date.none.fl_str_mv | 2025-11-25T05:11:30Z |
| dc.identifier.none.fl_str_mv | 10.3389/fonc.2025.1674849.s005 |
| dc.relation.none.fl_str_mv | https://figshare.com/articles/figure/Image_10_The_role_of_TMEM59L_in_colorectal_cancer_progression_and_its_interaction_with_the_TGF-_Smad_pathway_jpeg/30703493 |
| dc.rights.none.fl_str_mv | CC BY 4.0 info:eu-repo/semantics/openAccess |
| dc.subject.none.fl_str_mv | Oncology and Carcinogenesis not elsewhere classified colorectal neoplasms TMEM59L TGF-β signaling pathway metastasis therapeutic target |
| dc.title.none.fl_str_mv | Image 10_The role of TMEM59L in colorectal cancer progression and its interaction with the TGF-β/Smad pathway.jpeg |
| dc.type.none.fl_str_mv | Image Figure info:eu-repo/semantics/publishedVersion image |
| description | Objective<p>This study aimed to investigate the role of TMEM59L in colorectal cancer (CRC) and its interaction with the TGF-β/Smad signaling pathway.</p>Methods<p>We analyzed the correlation between TMEM59L expression levels and patient survival, as well as its impact on the TGF-β/Smad signaling pathway, using data from The Cancer Genome Atlas (TCGA). Additionally, transwell, CCK-8, EdU, and colony formation assays were conducted to assess the effects of TMEM59L on CRC cell migration, invasion, and proliferation. Gene silencing and overexpression, along with specific inhibitors/agonists, were used to validate the involvement of TMEM59L in the regulation of the TGF-β/Smad signaling pathway.</p>Results<p>We found that high TMEM59L expression was associated with poor patient survival and TGF-β pathway activation. After si-TMEM59L treatment, the migration and invasion abilities of CRC cells were reduced, while cell proliferation remained affected to a lesser extent. Additionally, the levels of TGF-β protein were decreased, and the phosphorylation of Smad2/3 was reduced. In vivo, TMEM59L knockdown reduced metastatic potential as demonstrated by decreased fluorescence intensity, while overexpression of TMEM59L increased metastatic potential, which was reversed by TGF-β inhibition.</p>Conclusion<p>TMEM59L may promote CRC metastasis by enhancing cell migration and invasion, with minimal impact on cell proliferation, potentially through the TGF-β/Smad signaling pathway.</p> |
| eu_rights_str_mv | openAccess |
| id | Manara_6c4a19e1c73dfc97410a74f554802234 |
| identifier_str_mv | 10.3389/fonc.2025.1674849.s005 |
| network_acronym_str | Manara |
| network_name_str | ManaraRepo |
| oai_identifier_str | oai:figshare.com:article/30703493 |
| publishDate | 2025 |
| repository.mail.fl_str_mv | |
| repository.name.fl_str_mv | |
| repository_id_str | |
| rights_invalid_str_mv | CC BY 4.0 |
| spelling | Image 10_The role of TMEM59L in colorectal cancer progression and its interaction with the TGF-β/Smad pathway.jpegHongjie Yang (383059)Jiafei Liu (7301984)Peishi Jiang (10110097)Yi Sun (118759)Lingyi Chen (796170)Siwei Zhu (400113)Oncology and Carcinogenesis not elsewhere classifiedcolorectal neoplasmsTMEM59LTGF-β signaling pathwaymetastasistherapeutic targetObjective<p>This study aimed to investigate the role of TMEM59L in colorectal cancer (CRC) and its interaction with the TGF-β/Smad signaling pathway.</p>Methods<p>We analyzed the correlation between TMEM59L expression levels and patient survival, as well as its impact on the TGF-β/Smad signaling pathway, using data from The Cancer Genome Atlas (TCGA). Additionally, transwell, CCK-8, EdU, and colony formation assays were conducted to assess the effects of TMEM59L on CRC cell migration, invasion, and proliferation. Gene silencing and overexpression, along with specific inhibitors/agonists, were used to validate the involvement of TMEM59L in the regulation of the TGF-β/Smad signaling pathway.</p>Results<p>We found that high TMEM59L expression was associated with poor patient survival and TGF-β pathway activation. After si-TMEM59L treatment, the migration and invasion abilities of CRC cells were reduced, while cell proliferation remained affected to a lesser extent. Additionally, the levels of TGF-β protein were decreased, and the phosphorylation of Smad2/3 was reduced. In vivo, TMEM59L knockdown reduced metastatic potential as demonstrated by decreased fluorescence intensity, while overexpression of TMEM59L increased metastatic potential, which was reversed by TGF-β inhibition.</p>Conclusion<p>TMEM59L may promote CRC metastasis by enhancing cell migration and invasion, with minimal impact on cell proliferation, potentially through the TGF-β/Smad signaling pathway.</p>2025-11-25T05:11:30ZImageFigureinfo:eu-repo/semantics/publishedVersionimage10.3389/fonc.2025.1674849.s005https://figshare.com/articles/figure/Image_10_The_role_of_TMEM59L_in_colorectal_cancer_progression_and_its_interaction_with_the_TGF-_Smad_pathway_jpeg/30703493CC BY 4.0info:eu-repo/semantics/openAccessoai:figshare.com:article/307034932025-11-25T05:11:30Z |
| spellingShingle | Image 10_The role of TMEM59L in colorectal cancer progression and its interaction with the TGF-β/Smad pathway.jpeg Hongjie Yang (383059) Oncology and Carcinogenesis not elsewhere classified colorectal neoplasms TMEM59L TGF-β signaling pathway metastasis therapeutic target |
| status_str | publishedVersion |
| title | Image 10_The role of TMEM59L in colorectal cancer progression and its interaction with the TGF-β/Smad pathway.jpeg |
| title_full | Image 10_The role of TMEM59L in colorectal cancer progression and its interaction with the TGF-β/Smad pathway.jpeg |
| title_fullStr | Image 10_The role of TMEM59L in colorectal cancer progression and its interaction with the TGF-β/Smad pathway.jpeg |
| title_full_unstemmed | Image 10_The role of TMEM59L in colorectal cancer progression and its interaction with the TGF-β/Smad pathway.jpeg |
| title_short | Image 10_The role of TMEM59L in colorectal cancer progression and its interaction with the TGF-β/Smad pathway.jpeg |
| title_sort | Image 10_The role of TMEM59L in colorectal cancer progression and its interaction with the TGF-β/Smad pathway.jpeg |
| topic | Oncology and Carcinogenesis not elsewhere classified colorectal neoplasms TMEM59L TGF-β signaling pathway metastasis therapeutic target |