Restoration of virulence in the natural avirulent strain of <i>B</i>. <i>glumae</i>, 257sh-1, by heterologous expression of <i>qsmR</i> from the virulent strain, 336gr-1.
<p>A) Restoration of toxoflavin production by a <i>qsmR</i> clone carrying the virulent allele, p<i>qsmR-1</i>. A <i>qsmR</i> clone carrying the avirulent allele, p<i>qsmR-A1</i>, did not restore toxoflavin production. The photo was taken 24 h af...
محفوظ في:
| المؤلف الرئيسي: | |
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| مؤلفون آخرون: | , , , , |
| منشور في: |
2024
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| الموضوعات: | |
| الوسوم: |
إضافة وسم
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| الملخص: | <p>A) Restoration of toxoflavin production by a <i>qsmR</i> clone carrying the virulent allele, p<i>qsmR-1</i>. A <i>qsmR</i> clone carrying the avirulent allele, p<i>qsmR-A1</i>, did not restore toxoflavin production. The photo was taken 24 h after inoculation and following incubation at 37°C. B) Restoration of virulence in the surrogate virulence assay system using onion scales by two independent <i>qsmR</i> clones carrying the virulent allele, p<i>qsmR-1</i> and p<i>qsmR-2</i>. A <i>qsmR</i> clone carrying the avirulent allele, p<i>qsmR-A1</i>, did not restore the virulence phenotype. The photos were taken 48 h after inoculation and following incubation at 30°C. The experiments for A) and B) were conducted more than three times, consistently yielding similar results. C&D) Restoration of virulence in rice panicles by p<i>qsmR-1</i>. The photo and the disease severity data were obtained 6 days after inoculation. The experiment with rice plants for panels C and D was conducted once in the greenhouse with six replications. 257sh-1(EV) and 257sh-1(p<i>qsmR-A1</i>) indicate the 257sh-1 carrying the empty vector, pBBR1MCS-5, and a clone of the avirulent <i>qsmR</i> allele, respectively. ‘Mock’ indicates the inoculation with the buffer (10 mM MgCl<sub>2</sub>) used for bacterial suspensions. E) A western blot image showing QsmR protein expression in the strains of <i>B</i>. <i>glumae</i>. The GST-tagged QsmR protein extracted from <i>E</i>. <i>coli</i> is included as the positive control. The Coomassie-stained polyacrylamide gel (left) indicates the amount of each protein sample transferred to the nitrocellulose membrane exhibiting the QsmR signal (right). The green arrows indicate the GST-tagged QsmR protein. The red and blue arrows point to the overexpressed QsmR from the <i>qsmR</i> clones and the native QsmR protein from the wild type <i>B</i>. <i>glumae</i> strains 336gr-1 and 257sh-1, respectively. The presented Western blot result represents three independent experiments that exhibited the same pattern.</p> |
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