ORF45 promotes cell migration during early primary infection.
<p>A-C. HUVECs were transduced with IRES-zsGreen empty vector, ORF45 WT- or F66A-expressing lentiviruses (MOI = 2). Forty-eight hours later, the cells were observed and recorded under fluorescence microscopy (A). A total of 5 × 10<sup>4</sup> cells/well were subjected to a transwel...
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| Được phát hành: |
2025
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| Những chủ đề: | |
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| Tóm tắt: | <p>A-C. HUVECs were transduced with IRES-zsGreen empty vector, ORF45 WT- or F66A-expressing lentiviruses (MOI = 2). Forty-eight hours later, the cells were observed and recorded under fluorescence microscopy (A). A total of 5 × 10<sup>4</sup> cells/well were subjected to a transwell migration assay with DMEM containing 20% FBS. Cells were left for migration for 24 h and then fixed, stained with crystal violet and recorded by microscopy (B). Migrated cell numbers in each field were counted, and the means were analyzed and are shown (C). D-E. HUVECs were infected with empty or ORF45- or F66A-expressing lentiviruses (MOI = 5). Twelve hours post infection, the cells were subjected to wound healing assays. Representative images are shown (D), and the cells that migrated into the closure per field were counted. The means were analyzed and are shown (E). F. HUVECs were infected with purified Bac16, STOP45 or ORF45F66A recombinant viruses (MOI = 10). Sixty hours after infection, the cells were subjected to a wound healing scratch assay, and representative images are shown.</p> |
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