ORF45 promotes cell migration during early primary infection.
<p>A-C. HUVECs were transduced with IRES-zsGreen empty vector, ORF45 WT- or F66A-expressing lentiviruses (MOI = 2). Forty-eight hours later, the cells were observed and recorded under fluorescence microscopy (A). A total of 5 × 10<sup>4</sup> cells/well were subjected to a transwel...
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2025
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| _version_ | 1849927641320128512 |
|---|---|
| author | Xiaojuan Li (351842) |
| author2 | Li Quan (177024) Rihong Zhou (22676690) Xiangpeng Liu (17898881) Ronit Sarid (186795) Ersheng Kuang (129028) |
| author2_role | author author author author author |
| author_facet | Xiaojuan Li (351842) Li Quan (177024) Rihong Zhou (22676690) Xiangpeng Liu (17898881) Ronit Sarid (186795) Ersheng Kuang (129028) |
| author_role | author |
| dc.creator.none.fl_str_mv | Xiaojuan Li (351842) Li Quan (177024) Rihong Zhou (22676690) Xiangpeng Liu (17898881) Ronit Sarid (186795) Ersheng Kuang (129028) |
| dc.date.none.fl_str_mv | 2025-11-24T18:34:23Z |
| dc.identifier.none.fl_str_mv | 10.1371/journal.ppat.1013737.g003 |
| dc.relation.none.fl_str_mv | https://figshare.com/articles/figure/ORF45_promotes_cell_migration_during_early_primary_infection_/30697700 |
| dc.rights.none.fl_str_mv | CC BY 4.0 info:eu-repo/semantics/openAccess |
| dc.subject.none.fl_str_mv | Microbiology Cell Biology Molecular Biology Biotechnology Immunology Developmental Biology Cancer Infectious Diseases Virology s2152a knockin abolish primary effusion lymphoma multicentric castleman disease kaposi &# 8217 mediated viral infection infected adherent cells differentiated b cells sequential cell detachment mediated kshv transmission kshv lytic replication adherent cells lytic replication viral transmission kshv transmission cell detachment b lymphocytes lytic cycle cell transmission taken together results demonstrated radically support natural donors key role key regulator etiological agent dependent manner cell contact cell adhesion associated herpesvirus |
| dc.title.none.fl_str_mv | ORF45 promotes cell migration during early primary infection. |
| dc.type.none.fl_str_mv | Image Figure info:eu-repo/semantics/publishedVersion image |
| description | <p>A-C. HUVECs were transduced with IRES-zsGreen empty vector, ORF45 WT- or F66A-expressing lentiviruses (MOI = 2). Forty-eight hours later, the cells were observed and recorded under fluorescence microscopy (A). A total of 5 × 10<sup>4</sup> cells/well were subjected to a transwell migration assay with DMEM containing 20% FBS. Cells were left for migration for 24 h and then fixed, stained with crystal violet and recorded by microscopy (B). Migrated cell numbers in each field were counted, and the means were analyzed and are shown (C). D-E. HUVECs were infected with empty or ORF45- or F66A-expressing lentiviruses (MOI = 5). Twelve hours post infection, the cells were subjected to wound healing assays. Representative images are shown (D), and the cells that migrated into the closure per field were counted. The means were analyzed and are shown (E). F. HUVECs were infected with purified Bac16, STOP45 or ORF45F66A recombinant viruses (MOI = 10). Sixty hours after infection, the cells were subjected to a wound healing scratch assay, and representative images are shown.</p> |
| eu_rights_str_mv | openAccess |
| id | Manara_6e8fd073fd5cc72236c1bc4772f62215 |
| identifier_str_mv | 10.1371/journal.ppat.1013737.g003 |
| network_acronym_str | Manara |
| network_name_str | ManaraRepo |
| oai_identifier_str | oai:figshare.com:article/30697700 |
| publishDate | 2025 |
| repository.mail.fl_str_mv | |
| repository.name.fl_str_mv | |
| repository_id_str | |
| rights_invalid_str_mv | CC BY 4.0 |
| spelling | ORF45 promotes cell migration during early primary infection.Xiaojuan Li (351842)Li Quan (177024)Rihong Zhou (22676690)Xiangpeng Liu (17898881)Ronit Sarid (186795)Ersheng Kuang (129028)MicrobiologyCell BiologyMolecular BiologyBiotechnologyImmunologyDevelopmental BiologyCancerInfectious DiseasesVirologys2152a knockin abolishprimary effusion lymphomamulticentric castleman diseasekaposi &# 8217mediated viral infectioninfected adherent cellsdifferentiated b cellssequential cell detachmentmediated kshv transmissionkshv lytic replicationadherent cellslytic replicationviral transmissionkshv transmissioncell detachmentb lymphocyteslytic cyclecell transmissiontaken togetherresults demonstratedradically supportnatural donorskey rolekey regulatoretiological agentdependent mannercell contactcell adhesionassociated herpesvirus<p>A-C. HUVECs were transduced with IRES-zsGreen empty vector, ORF45 WT- or F66A-expressing lentiviruses (MOI = 2). Forty-eight hours later, the cells were observed and recorded under fluorescence microscopy (A). A total of 5 × 10<sup>4</sup> cells/well were subjected to a transwell migration assay with DMEM containing 20% FBS. Cells were left for migration for 24 h and then fixed, stained with crystal violet and recorded by microscopy (B). Migrated cell numbers in each field were counted, and the means were analyzed and are shown (C). D-E. HUVECs were infected with empty or ORF45- or F66A-expressing lentiviruses (MOI = 5). Twelve hours post infection, the cells were subjected to wound healing assays. Representative images are shown (D), and the cells that migrated into the closure per field were counted. The means were analyzed and are shown (E). F. HUVECs were infected with purified Bac16, STOP45 or ORF45F66A recombinant viruses (MOI = 10). Sixty hours after infection, the cells were subjected to a wound healing scratch assay, and representative images are shown.</p>2025-11-24T18:34:23ZImageFigureinfo:eu-repo/semantics/publishedVersionimage10.1371/journal.ppat.1013737.g003https://figshare.com/articles/figure/ORF45_promotes_cell_migration_during_early_primary_infection_/30697700CC BY 4.0info:eu-repo/semantics/openAccessoai:figshare.com:article/306977002025-11-24T18:34:23Z |
| spellingShingle | ORF45 promotes cell migration during early primary infection. Xiaojuan Li (351842) Microbiology Cell Biology Molecular Biology Biotechnology Immunology Developmental Biology Cancer Infectious Diseases Virology s2152a knockin abolish primary effusion lymphoma multicentric castleman disease kaposi &# 8217 mediated viral infection infected adherent cells differentiated b cells sequential cell detachment mediated kshv transmission kshv lytic replication adherent cells lytic replication viral transmission kshv transmission cell detachment b lymphocytes lytic cycle cell transmission taken together results demonstrated radically support natural donors key role key regulator etiological agent dependent manner cell contact cell adhesion associated herpesvirus |
| status_str | publishedVersion |
| title | ORF45 promotes cell migration during early primary infection. |
| title_full | ORF45 promotes cell migration during early primary infection. |
| title_fullStr | ORF45 promotes cell migration during early primary infection. |
| title_full_unstemmed | ORF45 promotes cell migration during early primary infection. |
| title_short | ORF45 promotes cell migration during early primary infection. |
| title_sort | ORF45 promotes cell migration during early primary infection. |
| topic | Microbiology Cell Biology Molecular Biology Biotechnology Immunology Developmental Biology Cancer Infectious Diseases Virology s2152a knockin abolish primary effusion lymphoma multicentric castleman disease kaposi &# 8217 mediated viral infection infected adherent cells differentiated b cells sequential cell detachment mediated kshv transmission kshv lytic replication adherent cells lytic replication viral transmission kshv transmission cell detachment b lymphocytes lytic cycle cell transmission taken together results demonstrated radically support natural donors key role key regulator etiological agent dependent manner cell contact cell adhesion associated herpesvirus |