Expression and purification of rTsNas14 and enzyme activity assay.

<p>A. SDS-PAGE results for rTsNas14. Lane M: Protein marker. Lane 1: recombinant <i>E. coli</i> BL21 (DE3) pET-28a/TsNas14 lysate precipitation after 1 mM IPTG induction at 37°C; Lane 2: pET-28a/TsNas14 lysate before induction; Lane 3: pET-28a/TsNas14 lysate supernatant after induc...

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Hlavní autor:	Qingbo Lv (11631289) (author)
Další autoři:	Guangquan Si (22103868) (author), Chengyao Li (311264) (author), Ning Jiang (118848) (author), Yushu He (22103871) (author), Zijian Dong (501597) (author), Hanhai Mao (17087325) (author), Mingyuan Liu (142151) (author), Xiaolei Liu (104141) (author), Ying Zhao (16546) (author), Jing Ding (32381) (author)
Vydáno:	2025
Témata:	Biochemistry Cell Biology Genetics Molecular Biology Physiology Developmental Biology Cancer Science Policy Biological Sciences not elsewhere classified Chemical Sciences not elsewhere classified whole life cycle two shk domains tissue migration processes muscle larvae burden human colorectal adenocarcinoma vitro experiments showed zinc metalloproteinase nas quantitative results showed gelatin zymography showed novel potential target div >< p intestinal barrier model 6d aw stage 3d aw stage trichinella spiralis </ results showed trichinella </ spiralis </ intestinal barrier degrade gelatin 1 showed decreased aw zinc metalloproteinases vivo studies secretory products remains unclear primary objective natural activity molecular characterization may serve mainly distributed like proteinases highly conserved findings suggest enzyme activity effectively inhibited direct incubation crucial role adult worm
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Expression and purification of rTsNas14 and enzyme activity assay.

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