Disruption of organoid integrity and impaired forskolin response in PCOs infected with EBOV or MARV at high MOI.
<p>(A) At day 35 of differentiation, organoids were treated with forskolin at concentrations of 5, 10, and 20 µM (dissolved in DMSO) and imaged at 0 hours (top), 24 hours (middle), and 48 hours (bottom) to assess forskolin-induced swelling. Images were acquired using a Keyence BZ-X710 fluoresc...
محفوظ في:
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| مؤلفون آخرون: | , , , , , , , , , , , |
| منشور في: |
2025
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| _version_ | 1849927641269796864 |
|---|---|
| author | Elizabeth Y. Flores (22676699) |
| author2 | Adam J. Hume (6107039) Judith Olejnik (367849) Aditya Mithal (22676702) Andrew D’Amico (2248762) MengWei Yang (22676705) Pushpinder Bawa (733756) Feiya Wang (21075016) Aoife K. O’Connell (15462382) Anna Tseng (20843165) Nicholas A. Crossland (5500244) Gustavo Mostoslavsky (110612) Elke Mühlberger (45516) |
| author2_role | author author author author author author author author author author author author |
| author_facet | Elizabeth Y. Flores (22676699) Adam J. Hume (6107039) Judith Olejnik (367849) Aditya Mithal (22676702) Andrew D’Amico (2248762) MengWei Yang (22676705) Pushpinder Bawa (733756) Feiya Wang (21075016) Aoife K. O’Connell (15462382) Anna Tseng (20843165) Nicholas A. Crossland (5500244) Gustavo Mostoslavsky (110612) Elke Mühlberger (45516) |
| author_role | author |
| dc.creator.none.fl_str_mv | Elizabeth Y. Flores (22676699) Adam J. Hume (6107039) Judith Olejnik (367849) Aditya Mithal (22676702) Andrew D’Amico (2248762) MengWei Yang (22676705) Pushpinder Bawa (733756) Feiya Wang (21075016) Aoife K. O’Connell (15462382) Anna Tseng (20843165) Nicholas A. Crossland (5500244) Gustavo Mostoslavsky (110612) Elke Mühlberger (45516) |
| dc.date.none.fl_str_mv | 2025-11-24T18:35:06Z |
| dc.identifier.none.fl_str_mv | 10.1371/journal.ppat.1013698.s009 |
| dc.relation.none.fl_str_mv | https://figshare.com/articles/figure/Disruption_of_organoid_integrity_and_impaired_forskolin_response_in_PCOs_infected_with_EBOV_or_MARV_at_high_MOI_/30697757 |
| dc.rights.none.fl_str_mv | CC BY 4.0 info:eu-repo/semantics/openAccess |
| dc.subject.none.fl_str_mv | Biochemistry Microbiology Cell Biology Genetics Molecular Biology Physiology Biotechnology Immunology Cancer Mental Health Infectious Diseases Plant Biology Virology Biological Sciences not elsewhere classified Chemical Sciences not elsewhere classified xlink "> gastrointestinal support viral replication providing mechanistic insight induced secretory diarrhea colonic epithelial responses model ebola virus mediated ion transport human gi pathology ion transport severe diarrhea human ipsc stimulated genes specific induction powerful system pathogen interactions marburg virus junctional disruption including apical filovirus disease filoviral disease dissect host delayed virus colonic organoids central contributor |
| dc.title.none.fl_str_mv | Disruption of organoid integrity and impaired forskolin response in PCOs infected with EBOV or MARV at high MOI. |
| dc.type.none.fl_str_mv | Image Figure info:eu-repo/semantics/publishedVersion image |
| description | <p>(A) At day 35 of differentiation, organoids were treated with forskolin at concentrations of 5, 10, and 20 µM (dissolved in DMSO) and imaged at 0 hours (top), 24 hours (middle), and 48 hours (bottom) to assess forskolin-induced swelling. Images were acquired using a Keyence BZ-X710 fluorescence microscope. Scale bar = 500 µm. (B and C) PCOs were infected with (B) EBOV or (C) MARV at MOI 0.1, 10, or 100. At 1 dpi, organoids were treated with 5 µM forskolin and subsequently imaged at 0 hours (1 dpi), 24 hours (2 dpi), and 48 hours (3 dpi) post-treatment. Changes in organoid morphology, including swelling and structural integrity, were evaluated at each time point. Images were captured using the EVOS M5000 Imaging System. Scale bar = 500 µm.</p> <p>(TIF)</p> |
| eu_rights_str_mv | openAccess |
| id | Manara_71437cb265ddb67c68118134c7360b74 |
| identifier_str_mv | 10.1371/journal.ppat.1013698.s009 |
| network_acronym_str | Manara |
| network_name_str | ManaraRepo |
| oai_identifier_str | oai:figshare.com:article/30697757 |
| publishDate | 2025 |
| repository.mail.fl_str_mv | |
| repository.name.fl_str_mv | |
| repository_id_str | |
| rights_invalid_str_mv | CC BY 4.0 |
| spelling | Disruption of organoid integrity and impaired forskolin response in PCOs infected with EBOV or MARV at high MOI.Elizabeth Y. Flores (22676699)Adam J. Hume (6107039)Judith Olejnik (367849)Aditya Mithal (22676702)Andrew D’Amico (2248762)MengWei Yang (22676705)Pushpinder Bawa (733756)Feiya Wang (21075016)Aoife K. O’Connell (15462382)Anna Tseng (20843165)Nicholas A. Crossland (5500244)Gustavo Mostoslavsky (110612)Elke Mühlberger (45516)BiochemistryMicrobiologyCell BiologyGeneticsMolecular BiologyPhysiologyBiotechnologyImmunologyCancerMental HealthInfectious DiseasesPlant BiologyVirologyBiological Sciences not elsewhere classifiedChemical Sciences not elsewhere classifiedxlink "> gastrointestinalsupport viral replicationproviding mechanistic insightinduced secretory diarrheacolonic epithelial responsesmodel ebola virusmediated ion transporthuman gi pathologyion transportsevere diarrheahuman ipscstimulated genesspecific inductionpowerful systempathogen interactionsmarburg virusjunctional disruptionincluding apicalfilovirus diseasefiloviral diseasedissect hostdelayed viruscolonic organoidscentral contributor<p>(A) At day 35 of differentiation, organoids were treated with forskolin at concentrations of 5, 10, and 20 µM (dissolved in DMSO) and imaged at 0 hours (top), 24 hours (middle), and 48 hours (bottom) to assess forskolin-induced swelling. Images were acquired using a Keyence BZ-X710 fluorescence microscope. Scale bar = 500 µm. (B and C) PCOs were infected with (B) EBOV or (C) MARV at MOI 0.1, 10, or 100. At 1 dpi, organoids were treated with 5 µM forskolin and subsequently imaged at 0 hours (1 dpi), 24 hours (2 dpi), and 48 hours (3 dpi) post-treatment. Changes in organoid morphology, including swelling and structural integrity, were evaluated at each time point. Images were captured using the EVOS M5000 Imaging System. Scale bar = 500 µm.</p> <p>(TIF)</p>2025-11-24T18:35:06ZImageFigureinfo:eu-repo/semantics/publishedVersionimage10.1371/journal.ppat.1013698.s009https://figshare.com/articles/figure/Disruption_of_organoid_integrity_and_impaired_forskolin_response_in_PCOs_infected_with_EBOV_or_MARV_at_high_MOI_/30697757CC BY 4.0info:eu-repo/semantics/openAccessoai:figshare.com:article/306977572025-11-24T18:35:06Z |
| spellingShingle | Disruption of organoid integrity and impaired forskolin response in PCOs infected with EBOV or MARV at high MOI. Elizabeth Y. Flores (22676699) Biochemistry Microbiology Cell Biology Genetics Molecular Biology Physiology Biotechnology Immunology Cancer Mental Health Infectious Diseases Plant Biology Virology Biological Sciences not elsewhere classified Chemical Sciences not elsewhere classified xlink "> gastrointestinal support viral replication providing mechanistic insight induced secretory diarrhea colonic epithelial responses model ebola virus mediated ion transport human gi pathology ion transport severe diarrhea human ipsc stimulated genes specific induction powerful system pathogen interactions marburg virus junctional disruption including apical filovirus disease filoviral disease dissect host delayed virus colonic organoids central contributor |
| status_str | publishedVersion |
| title | Disruption of organoid integrity and impaired forskolin response in PCOs infected with EBOV or MARV at high MOI. |
| title_full | Disruption of organoid integrity and impaired forskolin response in PCOs infected with EBOV or MARV at high MOI. |
| title_fullStr | Disruption of organoid integrity and impaired forskolin response in PCOs infected with EBOV or MARV at high MOI. |
| title_full_unstemmed | Disruption of organoid integrity and impaired forskolin response in PCOs infected with EBOV or MARV at high MOI. |
| title_short | Disruption of organoid integrity and impaired forskolin response in PCOs infected with EBOV or MARV at high MOI. |
| title_sort | Disruption of organoid integrity and impaired forskolin response in PCOs infected with EBOV or MARV at high MOI. |
| topic | Biochemistry Microbiology Cell Biology Genetics Molecular Biology Physiology Biotechnology Immunology Cancer Mental Health Infectious Diseases Plant Biology Virology Biological Sciences not elsewhere classified Chemical Sciences not elsewhere classified xlink "> gastrointestinal support viral replication providing mechanistic insight induced secretory diarrhea colonic epithelial responses model ebola virus mediated ion transport human gi pathology ion transport severe diarrhea human ipsc stimulated genes specific induction powerful system pathogen interactions marburg virus junctional disruption including apical filovirus disease filoviral disease dissect host delayed virus colonic organoids central contributor |