PMA/I and 6F9 + 5B8 stimulation significantly reduces the proportion of Tregs.

After three days of stimulation with mitogen or plate-bound antibody (6F9=CA17.6F9; 2A12=CA17.2A12), PBMCs (n = 5) were removed from stimulation. Identification of Tregs was determined by surface expression of CD4 and CD25 and intracellular expression of Foxp3. Freque...

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Bibliographic Details
Main Author:	Treyvon W. Davis (22232198) (author)
Other Authors:	Jennifer C. Holmes (22232201) (author), Arissa He (22232204) (author), Paul R. Hess (17782693) (author), Christopher L. Mariani (11066799) (author), Yevgeny Brudno (503273) (author)
Published:	2025
Subjects:	Biochemistry Cell Biology Genetics Biotechnology Immunology Cancer Hematology Biological Sciences not elsewhere classified successfully troubleshooting issues phorbol myristate acetate cd3 clone ca17 cd28 clone 5b8 anyone directly compared improve patient outcomes div >< p optimal viral transduction stimulation strategies producing transduction </ p bound &# 945 &# 945 stimulation strategies bound antibodies better transduction cell stimulation cell transduction unique opportunity strategy affected solid neoplasms restricting advancements improve efficacy important model human cancers genetically modified far superior conjugated beads cona ). coated plates best results also examined
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PMA/I and 6F9 + 5B8 stimulation significantly reduces the proportion of T<sub>regs</sub>.

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