Deletion of the C-terminus of ATR leads to DNA damage and an increase of aberrant cells.
<p>(A - B) Western analysis of whole cell extracts from mycATR and mycATRΔC-/- cells. Cells were left untreated (NT) or treated with 0.5 mM of Hydroxyurea (HU) for 20 hours (Chronic) or treated with 5 mM of Hydroxyurea (HU) for 5 hours (Acute), washed and resuspended in fresh media. The sample...
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2025
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Cuir clib leis
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| _version_ | 1849927641776259072 |
|---|---|
| author | Gabriel L. A. da Silva (22676649) |
| author2 | Jeziel D. Damasceno (9073663) Jennifer A. Black (14411790) Craig Lapsley (6082646) Richard McCulloch (12918) Luiz R. O. Tosi (9073666) |
| author2_role | author author author author author |
| author_facet | Gabriel L. A. da Silva (22676649) Jeziel D. Damasceno (9073663) Jennifer A. Black (14411790) Craig Lapsley (6082646) Richard McCulloch (12918) Luiz R. O. Tosi (9073666) |
| author_role | author |
| dc.creator.none.fl_str_mv | Gabriel L. A. da Silva (22676649) Jeziel D. Damasceno (9073663) Jennifer A. Black (14411790) Craig Lapsley (6082646) Richard McCulloch (12918) Luiz R. O. Tosi (9073666) |
| dc.date.none.fl_str_mv | 2025-11-24T18:33:15Z |
| dc.identifier.none.fl_str_mv | 10.1371/journal.pgen.1011899.g004 |
| dc.relation.none.fl_str_mv | https://figshare.com/articles/figure/Deletion_of_the_C-terminus_of_ATR_leads_to_DNA_damage_and_an_increase_of_aberrant_cells_/30697539 |
| dc.rights.none.fl_str_mv | CC BY 4.0 info:eu-repo/semantics/openAccess |
| dc.subject.none.fl_str_mv | Biophysics Biochemistry Microbiology Cell Biology Genetics Molecular Biology Developmental Biology Science Policy Infectious Diseases Biological Sciences not elsewhere classified Physical Sciences not elsewhere classified major &# 8217 div >< p data reveals loss cells possess mechanisms remarkably plastic genome cas9 genome editing like family acts protein kinase ataxia atr depends upon delayed dna replication stranded dna accumulation leishmania major </ dna damage kinase dna damage genome stability leishmania </ dna metabolism dna injuries atr acts replication stress replication impediments work shows whose integrity terminal knockout smaller chromosomes protozoan parasite phosphatidylinositol 3 nuclear localisation mycatrδc -/-). mycatr ), master regulator larger chromosomes key role increased levels functional relevance eukaryotic response deletion results constantly challenged atr plays atr homolog atr ), |
| dc.title.none.fl_str_mv | Deletion of the C-terminus of ATR leads to DNA damage and an increase of aberrant cells. |
| dc.type.none.fl_str_mv | Image Figure info:eu-repo/semantics/publishedVersion image |
| description | <p>(A - B) Western analysis of whole cell extracts from mycATR and mycATRΔC-/- cells. Cells were left untreated (NT) or treated with 0.5 mM of Hydroxyurea (HU) for 20 hours (Chronic) or treated with 5 mM of Hydroxyurea (HU) for 5 hours (Acute), washed and resuspended in fresh media. The samples were collected at 0 and 5 hours after chronic treatment, and 0 and 4 hours after acute treatment. Blots were probed with α-γH2A antiserum; α-EIF1α was used as a loading control (predicted protein sizes are indicated, kDa). (C – D) Quantification of signal from the western blot showed in (A and B) using ImageJ software; statistical analysis was conducted using Graph Prism (Error bars ± SD; n = 3 experiments, * p < 0.05; ** p < 0.005; *** p < 0.001; **** p < 0.0001. Unpaired t-test). (E) Representative images acquired by a LSM880 Zeiss confocal microscope from Z stack images of mycATRΔC-/- (cl8) cells after release from chronic treatment (5 hours post treatment). Cells were fixed with 3% formaldehyde, genomic DNA stained with DAPI (cyan) and nucleus (n), kinetoplast (k) and micronucleus (n*) are indicated; α-tubulin was used to stain the cytoskeleton (gray). Bar = 12 um. (F) Percentage of populations with the indicted proportions of Nucleus/Kinetoplast (n/k) in mycATR and mycATRΔC-/- cells untreated (NT) or after release from chronic (5 hours post treatment) or acute (4 hours post treatment) HU treatment. The populations were coloured according to the proportion of n/k as normal (1n1k, 1n2n, 2n2k) or aberrant (0n1k, 1n0k, 2n1k, the presence of a micronucleus, 1M and Others for low aberrant cells as showed 0K2N). Error bars ± SD, n = 2 (>120 cells counted/experiment).</p> |
| eu_rights_str_mv | openAccess |
| id | Manara_71beb741b902baa2e40cf41f0841d33d |
| identifier_str_mv | 10.1371/journal.pgen.1011899.g004 |
| network_acronym_str | Manara |
| network_name_str | ManaraRepo |
| oai_identifier_str | oai:figshare.com:article/30697539 |
| publishDate | 2025 |
| repository.mail.fl_str_mv | |
| repository.name.fl_str_mv | |
| repository_id_str | |
| rights_invalid_str_mv | CC BY 4.0 |
| spelling | Deletion of the C-terminus of ATR leads to DNA damage and an increase of aberrant cells.Gabriel L. A. da Silva (22676649)Jeziel D. Damasceno (9073663)Jennifer A. Black (14411790)Craig Lapsley (6082646)Richard McCulloch (12918)Luiz R. O. Tosi (9073666)BiophysicsBiochemistryMicrobiologyCell BiologyGeneticsMolecular BiologyDevelopmental BiologyScience PolicyInfectious DiseasesBiological Sciences not elsewhere classifiedPhysical Sciences not elsewhere classifiedmajor &# 8217div >< pdata reveals losscells possess mechanismsremarkably plastic genomecas9 genome editinglike family actsprotein kinase ataxiaatr depends upondelayed dna replicationstranded dna accumulationleishmania major </dna damage kinasedna damagegenome stabilityleishmania </dna metabolismdna injuriesatr actsreplication stressreplication impedimentswork showswhose integrityterminal knockoutsmaller chromosomesprotozoan parasitephosphatidylinositol 3nuclear localisationmycatrδc -/-).mycatr ),master regulatorlarger chromosomeskey roleincreased levelsfunctional relevanceeukaryotic responsedeletion resultsconstantly challengedatr playsatr homologatr ),<p>(A - B) Western analysis of whole cell extracts from mycATR and mycATRΔC-/- cells. Cells were left untreated (NT) or treated with 0.5 mM of Hydroxyurea (HU) for 20 hours (Chronic) or treated with 5 mM of Hydroxyurea (HU) for 5 hours (Acute), washed and resuspended in fresh media. The samples were collected at 0 and 5 hours after chronic treatment, and 0 and 4 hours after acute treatment. Blots were probed with α-γH2A antiserum; α-EIF1α was used as a loading control (predicted protein sizes are indicated, kDa). (C – D) Quantification of signal from the western blot showed in (A and B) using ImageJ software; statistical analysis was conducted using Graph Prism (Error bars ± SD; n = 3 experiments, * p < 0.05; ** p < 0.005; *** p < 0.001; **** p < 0.0001. Unpaired t-test). (E) Representative images acquired by a LSM880 Zeiss confocal microscope from Z stack images of mycATRΔC-/- (cl8) cells after release from chronic treatment (5 hours post treatment). Cells were fixed with 3% formaldehyde, genomic DNA stained with DAPI (cyan) and nucleus (n), kinetoplast (k) and micronucleus (n*) are indicated; α-tubulin was used to stain the cytoskeleton (gray). Bar = 12 um. (F) Percentage of populations with the indicted proportions of Nucleus/Kinetoplast (n/k) in mycATR and mycATRΔC-/- cells untreated (NT) or after release from chronic (5 hours post treatment) or acute (4 hours post treatment) HU treatment. The populations were coloured according to the proportion of n/k as normal (1n1k, 1n2n, 2n2k) or aberrant (0n1k, 1n0k, 2n1k, the presence of a micronucleus, 1M and Others for low aberrant cells as showed 0K2N). Error bars ± SD, n = 2 (>120 cells counted/experiment).</p>2025-11-24T18:33:15ZImageFigureinfo:eu-repo/semantics/publishedVersionimage10.1371/journal.pgen.1011899.g004https://figshare.com/articles/figure/Deletion_of_the_C-terminus_of_ATR_leads_to_DNA_damage_and_an_increase_of_aberrant_cells_/30697539CC BY 4.0info:eu-repo/semantics/openAccessoai:figshare.com:article/306975392025-11-24T18:33:15Z |
| spellingShingle | Deletion of the C-terminus of ATR leads to DNA damage and an increase of aberrant cells. Gabriel L. A. da Silva (22676649) Biophysics Biochemistry Microbiology Cell Biology Genetics Molecular Biology Developmental Biology Science Policy Infectious Diseases Biological Sciences not elsewhere classified Physical Sciences not elsewhere classified major &# 8217 div >< p data reveals loss cells possess mechanisms remarkably plastic genome cas9 genome editing like family acts protein kinase ataxia atr depends upon delayed dna replication stranded dna accumulation leishmania major </ dna damage kinase dna damage genome stability leishmania </ dna metabolism dna injuries atr acts replication stress replication impediments work shows whose integrity terminal knockout smaller chromosomes protozoan parasite phosphatidylinositol 3 nuclear localisation mycatrδc -/-). mycatr ), master regulator larger chromosomes key role increased levels functional relevance eukaryotic response deletion results constantly challenged atr plays atr homolog atr ), |
| status_str | publishedVersion |
| title | Deletion of the C-terminus of ATR leads to DNA damage and an increase of aberrant cells. |
| title_full | Deletion of the C-terminus of ATR leads to DNA damage and an increase of aberrant cells. |
| title_fullStr | Deletion of the C-terminus of ATR leads to DNA damage and an increase of aberrant cells. |
| title_full_unstemmed | Deletion of the C-terminus of ATR leads to DNA damage and an increase of aberrant cells. |
| title_short | Deletion of the C-terminus of ATR leads to DNA damage and an increase of aberrant cells. |
| title_sort | Deletion of the C-terminus of ATR leads to DNA damage and an increase of aberrant cells. |
| topic | Biophysics Biochemistry Microbiology Cell Biology Genetics Molecular Biology Developmental Biology Science Policy Infectious Diseases Biological Sciences not elsewhere classified Physical Sciences not elsewhere classified major &# 8217 div >< p data reveals loss cells possess mechanisms remarkably plastic genome cas9 genome editing like family acts protein kinase ataxia atr depends upon delayed dna replication stranded dna accumulation leishmania major </ dna damage kinase dna damage genome stability leishmania </ dna metabolism dna injuries atr acts replication stress replication impediments work shows whose integrity terminal knockout smaller chromosomes protozoan parasite phosphatidylinositol 3 nuclear localisation mycatrδc -/-). mycatr ), master regulator larger chromosomes key role increased levels functional relevance eukaryotic response deletion results constantly challenged atr plays atr homolog atr ), |