Figure 4 from T Cells Promote Metastasis by Regulating Extracellular Matrix Remodeling following Chemotherapy
<p>Plasma from PTX-treated mice promotes pulmonary ECM remodeling. Tumor-free, 8- to 10-week-old BALB/c mice were treated with PTX or vehicle control. After 24 hours, blood was obtained by cardiac puncture and plasma was separated. The plasma was then injected intraperitoneally to naïve BALB/c...
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2025
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| Izvleček: | <p>Plasma from PTX-treated mice promotes pulmonary ECM remodeling. Tumor-free, 8- to 10-week-old BALB/c mice were treated with PTX or vehicle control. After 24 hours, blood was obtained by cardiac puncture and plasma was separated. The plasma was then injected intraperitoneally to naïve BALB/c mice (100 μL/mouse), and 72 hours later, lungs were removed (<i>n</i> = 4 mice/group). <b>A,</b> Lungs were analyzed for collagen content by Masson's trichrome staining. Representative images are shown with quantification of collagen staining per field (<i>n</i> > 4 fields/lung). Scale bar, 50 μm. <b>B,</b> The levels of collagen I, collagen IV, and LOX in lung sections were analyzed by immunostaining (red). Nuclei were stained with DAPI (blue). Scale bar, 50 μm. The percentage of red pixels per field was quantified (<i>n</i> > 4 fields/lung). <b>C,</b> Lung sections were also immunostained with antibodies against Collagen I and IV (shown in brown), and counterstained with hematoxylin. Representative images in two magnifications are shown. Scale bar, 200 μm. <b>D,</b> In a parallel experiment, collagen-coated plates were pretreated with plasma (10%) from control or PTX-treated mice for 4 hours, followed by washing. EMT6 cells (2 × 10<sup>4</sup> cells/well) were then seeded onto the plates, and after 15 minutes, nonadherent cells were removed. Adherent cells were stained with DAPI and analyzed by fluorescence microscopy. Representative images are shown on the left. Scale bar, 75 μm. Quantification of adherent cells is shown on the right. Statistical significance was assessed by unpaired two-tailed <i>t</i> test. Significant <i>P</i> values are shown as *, <i>P</i> < 0.05; **, <i>P</i> < 0.01; ***, <i>P</i> < 0.001.</p> |
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