DBLα-tag sequencing and immunity.

<p>(<b>A</b>) Gating strategy of surface recognition assay (SRA) with FCR3 iRBC incubated with plasma of pooled plasma of Malian individuals and stained with SYBRgreen, first gated on single cells based on forward and side scatter (top two small plots each panel) and then on infect...

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التفاصيل البيبلوغرافية
المؤلف الرئيسي:	Sukai Ceesay (3472853) (author)
مؤلفون آخرون:	Martin Kampmann (5688443) (author), Lasse Votborg-Novél (20138013) (author), Helle Smedegaard Hansson (21519864) (author), Rasmus Weisel Jensen (15216781) (author), Manuela Carrasquilla (2897933) (author), Hamidou Cisse (21519867) (author), Louise Turner (55117) (author), Usama Dabbas (21519870) (author), Christina Ntalla (20138016) (author), Silke Bandermann (3611189) (author), Safiatou Doumbo (248618) (author), Didier Doumtabe (248621) (author), Aissata Ongoiba (248615) (author), Kassoum Kayentao (248612) (author), Boubacar Traore (248609) (author), Peter D. Crompton (9462929) (author), Thomas Lavstsen (75520) (author), Silvia Portugal (3385946) (author)
منشور في:	2025
الموضوعات:	Microbiology Genetics Immunology Infectious Diseases Computational Biology Biological Sciences not elsewhere classified Physical Sciences not elsewhere classified used bioinformatic tools upstream sequence type main determinant limiting achieved remains unknown severe malaria cases malaria cases compared term infections may seasonal transmission areas clinical malaria occurs clinical malaria cases div >< p plasmodium falciparum </ month dry season binding pfemp1 dominated asymptomatic carriers expressed malaria transmission clinical cases asymptomatic carriers falciparum </ subclinical infections clinical presentation wet season dry season binding phenotype binding pfemp1 vars </ var </ wet seasons transcripts attributed transcript variation transcript levels sustain long significant difference shows circulation sequenced expressed reduced cytoadhesion prolonged circulation parasite ’ malian children lower proportions lower levels larger proportion infected erythrocytes immune selection host ’ host immunity higher titers gene expression expresses fewer domain composition
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الوصف
الملخص:	<p>(<b>A</b>) Gating strategy of surface recognition assay (SRA) with FCR3 iRBC incubated with plasma of pooled plasma of Malian individuals and stained with SYBRgreen, first gated on single cells based on forward and side scatter (top two small plots each panel) and then on infected RBC based on SYBRgreen staining in the FITC channel (bottom small plot each panel). Surface recognition was identified by staining with an anti-human IgG APC antibody and detected in the APC channel (big plot each panel). (<b>B</b>) Correlation of humoral immunity measured by SRA (left) and AMA1 ELISA (right) in plasmas from dry and wet season (startDry 19 n = 17, endDry n = 10, midWet n = 17, MAL n = 10) to participant age with color indicating timepoint. (<b>C</b>) Correlation of humoral immunity measured by and AMA1 ELISA (top) and SRA (bottom)in the same plasmas and breadth of recognition of var domains measured in Luminex. (<b>D</b>) Correlation between the different measures of humoral immunity and participant age. (<b>E</b>) Correlation of humoral immunity measured by AMA1 ELISA (left) and SRA (right) and parasitaemia.</p> <p>(TIF)</p>

DBLα-tag sequencing and immunity.

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