Structural Class I viroporin characteristics displayed by p33.

Structural Class I viroporin characteristics displayed by p33.

<p>A) The TMD of p33 compared with that of three known viroporins (IAV M2, HIV-1 Vpu, and SARS-CoV E) representing the underlined TMD, the short external domains (ED) and longer cytoplasmic domains (CD), with cytoplasmic TMD-adjacent basic residues colored in red. B) Western blot of the crude...

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Bibliografische gegevens
Hoofdauteur:	Vicken Aknadibossian (22676694) (author)
Andere auteurs:	Clare Stokes (816556) (author), Roger L. Papke (685990) (author), Hao Wei Teh (22676697) (author), Ying Wang (11406) (author), Svetlana Y. Folimonova (8786570) (author)
Gepubliceerd in:	2025
Onderwerpen:	Biophysics Biochemistry Medicine Microbiology Cell Biology Ecology Infectious Diseases Plant Biology Virology Biological Sciences not elsewhere classified substantial structural similarities ion channel properties ion channel activity form ion channels closterovirus tristezae </ alphainfluenzavirus influenzae </ div >< p citrus tristeza virus lowered membrane potentials host ion homeostasis discuss additional characteristics m2 protein encoded viroporin </ p plant virus viroporin plant virus viroporins plant virus xenopus </ viroporin encoded influenza virus viral proteins using two using confocal third report study represents significantly lagged research focusing present evidence first validation favorable environments electron microscopy cytoplasmic domains associated protein >), possesses
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p33 displays cytoplasmic features of Class I viroporins.
door: Vicken Aknadibossian (22676694)
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Functional characteristics shared among the CTV p33 and other known viroporins.
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Western blot of purified p33 protein transiently expressed in <i>N. benthamiana</i> displaying protein bands which correspond to the expected sizes of p33 monomers, dimers, and tetramers.
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TEM observations of the vesicular structures and cell wall-plasma membrane interface ultrastructure of <i>N. benthamiana</i> epidermal cells in p33-expressing and control cells.
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The ectopic expression of GFP:p33 induced the accumulation of GFP:p33-containing vesicles and MVBs in <i>N. benthamiana</i> epidermal cells.
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