<b>Morin alleviates fructose-driven disturbance of podocyte mitochondrial energy metabolism by inhibiting AMPD activity to improve glomerular injury</b>
<p dir="ltr">High consumption of fructose is a significant risk factor for glomerular podocyte injury. We reported a novel mechanism that high fructose increased the activity of adenosine 5’-monophosphate deaminase (AMPD) in the purine nucleotide cycle (PNC), which contributed to mit...
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2025
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| _version_ | 1849927631419473920 |
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| author | Luyi Huang (22583081) |
| author_facet | Luyi Huang (22583081) |
| author_role | author |
| dc.creator.none.fl_str_mv | Luyi Huang (22583081) |
| dc.date.none.fl_str_mv | 2025-11-25T13:44:34Z |
| dc.identifier.none.fl_str_mv | 10.6084/m9.figshare.30571082.v2 |
| dc.relation.none.fl_str_mv | https://figshare.com/articles/dataset/_b_Morin_alleviates_fructose-driven_disturbance_of_podocyte_mitochondrial_energy_metabolism_by_inhibiting_AMPD_activity_to_improve_glomerular_injury_b_/30571082 |
| dc.rights.none.fl_str_mv | CC BY 4.0 info:eu-repo/semantics/openAccess |
| dc.subject.none.fl_str_mv | Signal transduction purine nucleotide cycle podocyte injury mitochondrial energy metabolism processes |
| dc.title.none.fl_str_mv | <b>Morin alleviates fructose-driven disturbance of podocyte mitochondrial energy metabolism by inhibiting AMPD activity to improve glomerular injury</b> |
| dc.type.none.fl_str_mv | Dataset info:eu-repo/semantics/publishedVersion dataset |
| description | <p dir="ltr">High consumption of fructose is a significant risk factor for glomerular podocyte injury. We reported a novel mechanism that high fructose increased the activity of adenosine 5’-monophosphate deaminase (AMPD) in the purine nucleotide cycle (PNC), which contributed to mitochondrial dysfunction and activated glycolysis in podocyte injury. We discovered that the natural polyphenol morin significantly mitigated mitochondrial dysfunction and the activation of glycolysis in its attenuation of fructose-induced podocyte injury. Molecular docking results revealed a potential interaction between morin and AMPD2, indicating that morin might act as a competitive inhibitor of AMPD2. Confirmedly, morin suppressed the abnormally enhanced activity of AMPD in podocyte induced by high fructose. Similar to morin, silencing AMPD2 also alleviated mitochondrial dysfunction and activated glycolysis which mitigated podocyte injury. In high fructose diet-fed rats, morin remarkedly improved the podocyte foot process effacement and reduced urinary albumin to creatinine ratio (UACR). Consistently, morin reversed the downregulation of glomerular synaptopodin expression and decreased the activity of renal cortex AMPD in this animal model. In conclusion, morin alleviated high fructose-induced podocyte injury by inhibiting AMPD activity in PNC, suggesting that AMPD2 might be a potential therapeutic target for podocyte injury caused by high fructose intake. This study provides novel insights into the mechanisms of morin against mitochondrial energy disturbance in podocyte injury.</p> |
| eu_rights_str_mv | openAccess |
| id | Manara_79132a3c6bbc2129b0d5eedecc14c4f0 |
| identifier_str_mv | 10.6084/m9.figshare.30571082.v2 |
| network_acronym_str | Manara |
| network_name_str | ManaraRepo |
| oai_identifier_str | oai:figshare.com:article/30571082 |
| publishDate | 2025 |
| repository.mail.fl_str_mv | |
| repository.name.fl_str_mv | |
| repository_id_str | |
| rights_invalid_str_mv | CC BY 4.0 |
| spelling | <b>Morin alleviates fructose-driven disturbance of podocyte mitochondrial energy metabolism by inhibiting AMPD activity to improve glomerular injury</b>Luyi Huang (22583081)Signal transductionpurine nucleotide cyclepodocyte injurymitochondrial energy metabolism processes<p dir="ltr">High consumption of fructose is a significant risk factor for glomerular podocyte injury. We reported a novel mechanism that high fructose increased the activity of adenosine 5’-monophosphate deaminase (AMPD) in the purine nucleotide cycle (PNC), which contributed to mitochondrial dysfunction and activated glycolysis in podocyte injury. We discovered that the natural polyphenol morin significantly mitigated mitochondrial dysfunction and the activation of glycolysis in its attenuation of fructose-induced podocyte injury. Molecular docking results revealed a potential interaction between morin and AMPD2, indicating that morin might act as a competitive inhibitor of AMPD2. Confirmedly, morin suppressed the abnormally enhanced activity of AMPD in podocyte induced by high fructose. Similar to morin, silencing AMPD2 also alleviated mitochondrial dysfunction and activated glycolysis which mitigated podocyte injury. In high fructose diet-fed rats, morin remarkedly improved the podocyte foot process effacement and reduced urinary albumin to creatinine ratio (UACR). Consistently, morin reversed the downregulation of glomerular synaptopodin expression and decreased the activity of renal cortex AMPD in this animal model. In conclusion, morin alleviated high fructose-induced podocyte injury by inhibiting AMPD activity in PNC, suggesting that AMPD2 might be a potential therapeutic target for podocyte injury caused by high fructose intake. This study provides novel insights into the mechanisms of morin against mitochondrial energy disturbance in podocyte injury.</p>2025-11-25T13:44:34ZDatasetinfo:eu-repo/semantics/publishedVersiondataset10.6084/m9.figshare.30571082.v2https://figshare.com/articles/dataset/_b_Morin_alleviates_fructose-driven_disturbance_of_podocyte_mitochondrial_energy_metabolism_by_inhibiting_AMPD_activity_to_improve_glomerular_injury_b_/30571082CC BY 4.0info:eu-repo/semantics/openAccessoai:figshare.com:article/305710822025-11-25T13:44:34Z |
| spellingShingle | <b>Morin alleviates fructose-driven disturbance of podocyte mitochondrial energy metabolism by inhibiting AMPD activity to improve glomerular injury</b> Luyi Huang (22583081) Signal transduction purine nucleotide cycle podocyte injury mitochondrial energy metabolism processes |
| status_str | publishedVersion |
| title | <b>Morin alleviates fructose-driven disturbance of podocyte mitochondrial energy metabolism by inhibiting AMPD activity to improve glomerular injury</b> |
| title_full | <b>Morin alleviates fructose-driven disturbance of podocyte mitochondrial energy metabolism by inhibiting AMPD activity to improve glomerular injury</b> |
| title_fullStr | <b>Morin alleviates fructose-driven disturbance of podocyte mitochondrial energy metabolism by inhibiting AMPD activity to improve glomerular injury</b> |
| title_full_unstemmed | <b>Morin alleviates fructose-driven disturbance of podocyte mitochondrial energy metabolism by inhibiting AMPD activity to improve glomerular injury</b> |
| title_short | <b>Morin alleviates fructose-driven disturbance of podocyte mitochondrial energy metabolism by inhibiting AMPD activity to improve glomerular injury</b> |
| title_sort | <b>Morin alleviates fructose-driven disturbance of podocyte mitochondrial energy metabolism by inhibiting AMPD activity to improve glomerular injury</b> |
| topic | Signal transduction purine nucleotide cycle podocyte injury mitochondrial energy metabolism processes |