Image 2_A metaproteomic analysis of the piglet fecal microbiome across the weaning transition.tif
<p>Microbiome analysis has relied largely on metagenomics to characterize microbial populations and predict their functions. Here, we used a metaproteomic analysis of the fecal microbiome in piglets before and after weaning to compare protein abundances as they pertain to microbial populations...
Saved in:
| Main Author: | |
|---|---|
| Other Authors: | , , , , , , , , , , , , , |
| Published: |
2025
|
| Subjects: | |
| Tags: |
Add Tag
No Tags, Be the first to tag this record!
|
| _version_ | 1852020832035930112 |
|---|---|
| author | Israel Rivera (3471599) |
| author2 | KaLynn Harlow (5236988) Robert N. Cole (82811) Robert O’Meally (789845) Wesley Garrett (1397437) Weili Xiong (1348971) William Oliver (5337962) James E. Wells (11257785) Katie Lynn Summers (7473512) Nisan Chhetri (21236264) Olga Postnikova (6998345) Lea Rempel (21236267) Matt Crouse (21236270) Bryan Neville (21236273) Cary Pirone Davies (14261792) |
| author2_role | author author author author author author author author author author author author author author |
| author_facet | Israel Rivera (3471599) KaLynn Harlow (5236988) Robert N. Cole (82811) Robert O’Meally (789845) Wesley Garrett (1397437) Weili Xiong (1348971) William Oliver (5337962) James E. Wells (11257785) Katie Lynn Summers (7473512) Nisan Chhetri (21236264) Olga Postnikova (6998345) Lea Rempel (21236267) Matt Crouse (21236270) Bryan Neville (21236273) Cary Pirone Davies (14261792) |
| author_role | author |
| dc.creator.none.fl_str_mv | Israel Rivera (3471599) KaLynn Harlow (5236988) Robert N. Cole (82811) Robert O’Meally (789845) Wesley Garrett (1397437) Weili Xiong (1348971) William Oliver (5337962) James E. Wells (11257785) Katie Lynn Summers (7473512) Nisan Chhetri (21236264) Olga Postnikova (6998345) Lea Rempel (21236267) Matt Crouse (21236270) Bryan Neville (21236273) Cary Pirone Davies (14261792) |
| dc.date.none.fl_str_mv | 2025-05-02T10:52:05Z |
| dc.identifier.none.fl_str_mv | 10.3389/fmicb.2025.1504433.s005 |
| dc.relation.none.fl_str_mv | https://figshare.com/articles/figure/Image_2_A_metaproteomic_analysis_of_the_piglet_fecal_microbiome_across_the_weaning_transition_tif/28919900 |
| dc.rights.none.fl_str_mv | CC BY 4.0 info:eu-repo/semantics/openAccess |
| dc.subject.none.fl_str_mv | Microbiology metaproteome microbiome weaning digestion swine |
| dc.title.none.fl_str_mv | Image 2_A metaproteomic analysis of the piglet fecal microbiome across the weaning transition.tif |
| dc.type.none.fl_str_mv | Image Figure info:eu-repo/semantics/publishedVersion image |
| description | <p>Microbiome analysis has relied largely on metagenomics to characterize microbial populations and predict their functions. Here, we used a metaproteomic analysis of the fecal microbiome in piglets before and after weaning to compare protein abundances as they pertain to microbial populations specific to either a milk- or plant-based diet. Fecal samples were collected from six piglets on the day of weaning and 4 weeks after transitioning to a standard nursery diet. Using the 12,554 protein groups identified in samples, we confirmed the shift in protein composition that takes place in response to the microbial succession following weaning and demonstrated the redundancy in metabolic processes between taxa. We identified taxa with roles as primary degraders based on corresponding proteins synthesized, thereby providing evidence for cross-feeding. Proteins associated with the breakdown of milk-specific carbohydrates were common among pre-weaned pigs, whereas the proteome of post-weaned piglets contained a greater abundance of proteins involved in the breaking down plant-specific carbohydrates. Furthermore, output revealed that production of propionate takes place via the propionaldehyde pathway in pre-weaned piglets, but changes to production via the succinate pathway in post-weaned piglets. Finally, a disproportionate quantity of carbohydrate-active enzymes (CAZymes) (~8%) were produced by fungi, which typically only represent ~0.1% of the microbiome taxa. Information gathered through this characterization of the metaproteome before and after weaning revealed important differences regarding the role of members in the microbial community, thereby providing information for the optimization of diets and products for both piglet and microbiome health.</p> |
| eu_rights_str_mv | openAccess |
| id | Manara_7b025e34646e07794fe8d8d80aa439dd |
| identifier_str_mv | 10.3389/fmicb.2025.1504433.s005 |
| network_acronym_str | Manara |
| network_name_str | ManaraRepo |
| oai_identifier_str | oai:figshare.com:article/28919900 |
| publishDate | 2025 |
| repository.mail.fl_str_mv | |
| repository.name.fl_str_mv | |
| repository_id_str | |
| rights_invalid_str_mv | CC BY 4.0 |
| spelling | Image 2_A metaproteomic analysis of the piglet fecal microbiome across the weaning transition.tifIsrael Rivera (3471599)KaLynn Harlow (5236988)Robert N. Cole (82811)Robert O’Meally (789845)Wesley Garrett (1397437)Weili Xiong (1348971)William Oliver (5337962)James E. Wells (11257785)Katie Lynn Summers (7473512)Nisan Chhetri (21236264)Olga Postnikova (6998345)Lea Rempel (21236267)Matt Crouse (21236270)Bryan Neville (21236273)Cary Pirone Davies (14261792)Microbiologymetaproteomemicrobiomeweaningdigestionswine<p>Microbiome analysis has relied largely on metagenomics to characterize microbial populations and predict their functions. Here, we used a metaproteomic analysis of the fecal microbiome in piglets before and after weaning to compare protein abundances as they pertain to microbial populations specific to either a milk- or plant-based diet. Fecal samples were collected from six piglets on the day of weaning and 4 weeks after transitioning to a standard nursery diet. Using the 12,554 protein groups identified in samples, we confirmed the shift in protein composition that takes place in response to the microbial succession following weaning and demonstrated the redundancy in metabolic processes between taxa. We identified taxa with roles as primary degraders based on corresponding proteins synthesized, thereby providing evidence for cross-feeding. Proteins associated with the breakdown of milk-specific carbohydrates were common among pre-weaned pigs, whereas the proteome of post-weaned piglets contained a greater abundance of proteins involved in the breaking down plant-specific carbohydrates. Furthermore, output revealed that production of propionate takes place via the propionaldehyde pathway in pre-weaned piglets, but changes to production via the succinate pathway in post-weaned piglets. Finally, a disproportionate quantity of carbohydrate-active enzymes (CAZymes) (~8%) were produced by fungi, which typically only represent ~0.1% of the microbiome taxa. Information gathered through this characterization of the metaproteome before and after weaning revealed important differences regarding the role of members in the microbial community, thereby providing information for the optimization of diets and products for both piglet and microbiome health.</p>2025-05-02T10:52:05ZImageFigureinfo:eu-repo/semantics/publishedVersionimage10.3389/fmicb.2025.1504433.s005https://figshare.com/articles/figure/Image_2_A_metaproteomic_analysis_of_the_piglet_fecal_microbiome_across_the_weaning_transition_tif/28919900CC BY 4.0info:eu-repo/semantics/openAccessoai:figshare.com:article/289199002025-05-02T10:52:05Z |
| spellingShingle | Image 2_A metaproteomic analysis of the piglet fecal microbiome across the weaning transition.tif Israel Rivera (3471599) Microbiology metaproteome microbiome weaning digestion swine |
| status_str | publishedVersion |
| title | Image 2_A metaproteomic analysis of the piglet fecal microbiome across the weaning transition.tif |
| title_full | Image 2_A metaproteomic analysis of the piglet fecal microbiome across the weaning transition.tif |
| title_fullStr | Image 2_A metaproteomic analysis of the piglet fecal microbiome across the weaning transition.tif |
| title_full_unstemmed | Image 2_A metaproteomic analysis of the piglet fecal microbiome across the weaning transition.tif |
| title_short | Image 2_A metaproteomic analysis of the piglet fecal microbiome across the weaning transition.tif |
| title_sort | Image 2_A metaproteomic analysis of the piglet fecal microbiome across the weaning transition.tif |
| topic | Microbiology metaproteome microbiome weaning digestion swine |